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EDITORIAL: AGEING AND SENESCENCE

Author: Haider S Kadhim حيدر صباح كاظم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 2 Pages: 102-104
Publisher: Al-Nahrain University جامعة النهرين

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EDITORIAL: TAKE CARE OF JOURNAL WITH FAKE IMPACT FACTOR

Author: Haider S. Kadhim حيدر صباح كاظم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2014 Volume: 12 Issue: 1 Pages: 1-3
Publisher: Al-Nahrain University جامعة النهرين

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EXPRESSION OF P53 PROTEIN IN NEOPLASTIC AND NON NEOPLASTIC OVARIAN LESIONS

Authors: Fahem M. Mahmood فاهم محسن محمود --- Haider S. Kadhim حيدر صباح كاظم --- Liqaa R. Al Khuzaee لقاء رياض موسى الخزاعي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 3 Pages: 280-284
Publisher: Al-Nahrain University جامعة النهرين

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Background:Ovarian cancer is one of the most common causes of gynecologic neoplasm all over the world.Objective:The objective is to shed light on the role of p53 protein and patient`s age in the pathogenesis of ovarian lesions.Methods:Paraffin embedded blocks of 62 patients with ovarian lesions were studied. Thirty-five cases of surface epithelial ovarian tumors, (31 cases of invasive surface epithelial ovarian tumors, and 4 cases of borderline intermediate malignancy cases of neoplastic ovarian cystic lesions). In addition, eighteen cases of benign neoplastic ovarian cystic lesions and 9 cases of non- neoplastic functional one were enrolled in this study. All of cases included, were stained with p53 by immunohistochemistry.Results:Immunohistochemistry for p53 showed that malignant cases were positive for p53 while all benign cases were negative for p53 and the borderline cases were also negative for p53. The non-neoplastic cases were negative for p53. There is a significant statistical difference in P53 expression in malignant cases compared to other groups (P < 0.001). A significant difference in mean age of malignant and border line cases in comparison with benign and non-neoplastic cases; (P < 0.001).Conclusion:Protein p53 may play a role in the pathogenesis of malignant ovarian cancer but not in benign lesions. The age of the patient has a role as a risk factor in ovarian lesions.Keywords:Ovarian lesion, ovarian cancer, p53, immunohistochemistry.


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5.SERO-PREVALENCE AND PLASMA VIRAL LOAD OF EPSTEIN BARR VIRUS AMONG IRAQI BLOOD DONORS

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Background:Epstein-Barr virus (EBV) is one of the most common latent viruses inside the humans' B-lymphocytes and it has been documented as a causative agent of many cancers. The virus may be transmitted when infected blood transfused to immunocompromised as well as immunocompetent individuals.Objective:To estimate the prevalence of EBV among apparently healthy blood donors by enzyme-linked immunosorbent assay (ELISA) and by quantitative real time polymerase chain reaction (RT-PCR).Methods:Four hundred fifty (450) blood donors were enrolled in this study. Plasma samples were screened by ELISA technique for detection of EBV viral capsid antigen (VCA-IgG). DNA extracted from 50 representative samples of these 450, and plasma EBV viral load was investigated by RT-PCR.Results:The overall sero-prevalence of EBV IgG was 79.8%, with a significantly higher prevalence among females than males. RT-PCR results were negative for all of the 50 representative samples.Conclusion:The high EBV sero-prevalence rates among Iraqi subjects raise the possibility of increasing the risk of EBV-associated malignant diseases.Keywords:Epstein-Barr virus, seroprevalence, VCA-IgG, real-time PCR, blood donorsCitation:Amjad Q. Redha, Asmaa B. Al-Obaidi, Haider F. Ghazi, Haider S. Kadhim. Sero-prevalence and plasma viral load of Epstein Barr virus among Iraqi blood donors. Iraqi JMS. 2017; Vol. 15(2): 135-142. doi: 10.22578/IJMS.15.2.5


Article
10.THE ROLE OF ATG16L1 (THR300ALA) GENETIC VARIANTS AND AUTOPHAGY IN DEVELOPMENT OF ACUTE AND CHRONIC URINARY TRACT INFECTION CAUSED BY UROPATHOGENIC ESCHERICHIA COLI

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Background: Uropathogenic Escherichia coli (UPEC) is the major cause of urinary tract infection (UTI), establish quiescent intracellular bacterial reservoirs (QIRs). These latent reservoirs, which persist indefinitely, are resistance to antibiotic therapies and can induce recurrence. Autophagy related 16 like 1 gene (ATG16L1) Thr300Ala genetic variant confer an increased risk for the development of urinary tract infection caused by UPEC. This study aimed.Objective: To determine the possible relationship between the ATG16L1 Thr300Ala genetic variant and UPEC for the development of acute and chronic urinary tract infection.Methods: A total of 100 urine and blood samples were collected from patients complain from UTI, 20 blood samples of apparently healthy during the period between (November 2014 to May 2015) from two hospitals in Baghdad; Al-Imamein Al-Kadhimein Medical City and Al-Yarmauk Teaching Hospital. The age range exactly with mean ± SD or SE. E. coli were isolated by ordinary methods and the identification of non entero-pathogenic E. coli was performed at a group level by slide agglutination test with specific antisera. UPEC isolates were tested for their susceptibility to 12 antimicrobial agents by disc-diffusion method. ATG16L1 T300A genotyping was done by Species Specific Primer – Polymerase Chain Reaction (SSP-PCR), after genomic DNA extraction from each blood sample. Results: A total of sixty E. coli were isolated from 20 acute UTI (16 females and 4 males) and 40 chronic UTI (32 females and 8 males). There is a high rate of acute UTI among the age range exactly groups (≤10-20 years) and chronic UTI among age groups (21-40 years). Overall isolates had a complete resistance to Ampicillin and Gentamycin (100%), high resistance to Nalidixic acid (88%), Piperacillin (86%), Trimethoprime+Sulfa (84%), Cefotriaxon (80%), Ciprofloxacin (78%) and Cephalosporin (66%). Moderate resistance to Azithromycin (51%) and Cephalothin (50%) were seen. Whereas these isolates were highly susceptible to Imipenem and Nitrofuranton with the resistance rate 8% & 27% respectively. Ninety percent of the isolates were resistant to three or more antibiotics. The SSP-PCR result showed that a (89%) and (92%) of acute positive E. coli infection (P=0.009) and chronic positive E. coli infection (P=0.006) respectively were carried allele-G. While the occurrence of allele-G was (62%) in acute negative E. coli infection, and (30%) in chronic negative E. coli infection.Conclusion: There is a relationship between allelic variants of ATG16L1 gene with acute and chronic UTI. In the other hands, the risk of the present allele G was associated with increased susceptibility to infection by UPEC, which developing chronic and acute UTI.Keywords: Escherichia coli, autophagy, Thr300Ala genetic variant, PCRCitation: Abd GH, Abd Al-Rahman TR, Ghazi HF, Kadhim HS. The role of ATG16L1 (Thr300Ala) genetic variants and autophagy in development of acute and chronic urinary tract infection caused by uropathogenic Escherichia coli. Iraqi JMS. 2017; Vol. 15(4). 396-403. doi: 10.22578/IJMS.15.4.10


Article
DETECTION OF EPSTEIN BARR VIRUS IN RENAL TRANSPLANT RECIPIENTS: TWO CENTERS STUDY

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Background:Viruses are among the most common causes of opportunistic infections after transplantation. The risk for viral infection is a function of the specific virus encountered and the intensity of immune suppression used to prevent graft rejection.Epstein-Barr virus infection has also been implicated as co-factor in acute and chronic rejection syndromes.Objective:Detection of Epstein-Barr viremia in renal transplant recipients.Methods:Fifty seven (57) renal transplant recipients were enrolled in this study. Plasma samples were taken from all renal transplant subjects. Screening of Epstein-Barr virus was first done by serology viamono spot test, then, viral DNA of Epstein-Barr viruswas extracted from 200 µl plasma samples and Epstein-Barr virus DNA was detected and measured by Taqman quantitative real-time PCR.Results:19/57 (33 %) of renal transplant subjects had Epstein-Barr virus viremia and the viral load ranged from 7100 to 16.165 copies/ml. Serology of all RT subjects showed negative heterophil antibody except for one patient had positive hetrophil antibody.Conclusion:The current study showed that Epstein-Barr virus might be considered as an important cause of renal impairment and allograft loss in renaltransplant subjects. And Epstein-Barr virus seems associated with post transplantation renal impairment and/or kidney rejection. Real-time PCR is a very sensitive and specific method for the detection of Epstein-Barr viremia in renal transplant subjects.Key words:Epstein-Barr virus, Renal transplantation, real-time PCR


Article
HUMAN CYTOMEGALOVIRUS INFECTION AMONG NEONATES WITH SYMPTOMATIC CONGENITAL INFECTIONS AND BIRTH DEFECTS

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Background:Human cytomegalovirus (HCMV) is the major viral etiology of congenital infection and birth defects, during current maternal infection the fetal transmission is high (30-40%) and the symptomatic neonates have diseases involving the neurologic, hematopoietic, respiratory and other organ systems, causing high mortality and long-term sequelae.Objective:To measure the frequency of congenital and perinatal HCMV infection among symptomatic neonates and its possible burden of disease among them.Methods:A total of one hundred ninety-eight symptomatic neonates with clinical manifestations of overt congenital infection enrolled in this study from September 2014 to March 2015. Serum samples were obtained from each subject targeted in this study. HCMV infection was defined as HCMV-IgM antibody positive by Electrochemiluminescence Immunoassay (ECLIA) techniques. Results:The prevalence of HCMV infection among symptomatic neonates with congenital infection was 25 (12.6%). The average age of HCMV detection was 9.96 (SD 6.73) days with a median of 7 days, a minimum of 3 days and a maximum of 28 days. Jaundice was the most predominant clinical finding 14 (56%), followed in order of frequency by hepatomegaly 9 (36%) and pneumonitis 7 (28%).Conclusion:The high prevalence of neonatal HCMV infection among neonates with symptomatic congenital infections could indicate a high rate of maternal HCMV primary or current infection among our population.Keywords: HCMV, congenital infection, neonates, clinical finding.


Article
2.HIGH PREVALENCE OF JOHN CUNNINGHAM VIRURIA IN RENAL TRANSPLANT RECIPIENTS

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Background:John Cunningham virus (JCV) is one of the important viruses in immunocompromised patients. High JC viruria is seen in kidney allograft recipients; some of them with a polyomavirus associated nephropathy (PVAN) just like BK polyomavirus but to a lesser extent.Objective:To detect JCV viruria in a sample of Iraqi renal transplant recipients, and its association with renal function.Methods:A prospective study enrolled 71 renal transplant recipients (RTR) and 20 normal donors (ND) as controls. Urine samples were collected from all RTR and ND. Viral DNA was extracted from 1 ml urine samples, and then, JC virus DNA was detected and measured by Taqman quantitative real-time PCR.Results:Out of 71 RTRs, 31 (43.66%), and 2 out of the 20 (10%) controls had positive JC viruria. The mean JCV viruria was 6.8 x104, and 1.04x103 copies/ml for RTRs and controls respectively.Conclusion:There is a relatively high prevalence of JCV viruria in Iraqi RTR patients.Keywords:JC virus, renal transplantation, urine, real-time PCR. Citation:Mervit B. Jasim, Ali J.H. Al-Saedi, Mustafa R. Hussein, Asmaa B. Al-Obaidi, Haider S. Kadhim. High prevalence of John Cunningham viruria in renal transplant recipients. Iraqi JMS. 2017; Vol. 15(2): 108-115. doi: 10.22578/IJMS.15.2.2


Article
BK POLYOMAVIRUS-INFECTED DECOY CELLS IN URINE CYTOLOGY SPECIMENS OF RENAL TRANSPLANT RECIPIENTS

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Background:BK polyomavirus is one of the common post-transplant viral infections, affecting ∼15% of renal transplantation recipients (RTR), leading to graft loss in more than half of cases.Objectives:Study the rate of detection of BK virus (BKV) in RTRs in Pap-stained urine cytology specimens.Methods:A single center study, urine samples were collected from 99 RTR patients, with 15 Living Donors (LD) and 15 patients with chronic kidney disease (CKD) were taken as controls. And urine cytology smears were Pap stained for detection of decoy cells (DCs).Results:Out of the 99 RTRs, 27 (27.3%) patients were decoy positive, 8 out of these 27 patients had uncommon DCs, and 5 out of these 27 cytology positive patients (18.5%) had biopsy proven BKV nephropathy (BKVN).Conclusion:This study suggests that the finding of BKVN in 18.5% of the DC positive patients stresses the importance of screening for BK polyomavirus with Pap-stained urinary cytology in RTR.Key wards:BK polyomavirus, renal transplantation, decoy cells

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