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Extraction,Purification and Characterization of Biosurfactant from Bacillus subtilis
Bacillus subtilis من بكتريا Biosurfactant تنقية وتوصيف الـ,أستخلاص

Authors: Ghofran Flaih Abd-Al-Hussan1 --- Fatima Abd AL–Hussein Mejbel --- Hamid Majid Jassim
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2016 Volume: 14 Issue: 2 Pages: 186-192
Publisher: Kerbala University جامعة كربلاء

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Abstract

Bacteria was isolated from soil sample of various automobile work shop of five different areas in Iraq.About 50 isolate suspected to belong to genus Bacillus.All these isolates were subjected to microscopic examination and cultural characteristics about 42 isolate belonged to genus B.subtilis. Crude biosurfactants produced by the selected isolates were extracted by acid precipitation followed by partially purified with dichloromethane.Biosurfactant has been purified from B.subtilis S4 by two purification steps included Ion exchange chromatography using DEAE- Cellulose followed by Gel filtration chromatography using Sepharose-6B.The the characteristics of the purification compouned were studied . Results showed that the molecular weight of biosurfactant produced by B.subtilis S4 ranging between (1036-1058 Da) with the HPLC-MS System .

تم عزل البكتريا من عينات التربه من مختلف محلات تصليح السيارات لخمس مناطق مختلفه من العراق وقد حصل على 50عزلة يتوقع انها عائدة الى جنس Bacillus وهذه العزلات تم اخضاعها الى الفحص المجهري واختبارات الصفات الزرعيه وقد حصل على 42 عزله تعود الى B.subtilis . تم انتاج Biosurfactant من العزلات المختاره وتم استخلاصه بواسطه الترسيب بالحامض وتبعها تنقيته جزئيا مع مذيب dichloromethane . تم تنقيه Biosurfactant من العزله B.subtilis S4 بواسطه طريقتين من التنقيه وهما Ion exchang chromatograghyبأستخدام DEAE-Cellulose وطريقه Gel filtration chromatograghy بأستخدام . Sepharose -6Bتم دراسة صفات المركب المنقى , واظهرت النتائج ان الوزن الجزيئي للـ Biosurfactant المنتج من العزله B.subtilis S4 يتراوح بين 1058-1036دالتون بواسطه HPLC-MS System .

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