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Article
PATTERN OF RESPONSES TO HEPATITIS B VIRUS VACCINE IN BASRAH, IRAQ

Authors: Firas T. Mansour --- Hassan J. Hasony
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2009 Volume: 27 Issue: 1 Pages: 28-33
Publisher: Basrah University جامعة البصرة

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ABSTRACTA serological study was carried out in Basrah governorate, southern Iraq, from October 2004 to the end ofSeptember2006 aimed at estimating the prevalence of HBs-antibody among four groups of individuals: Childrenunder 15 years of age, medical personnel, barbers and unvaccinated adults, to determine HBV vaccination coveragein our area, also to evaluate the duration of vaccine induced immunity and the rate of waning immunity as well asthe determination of the proportion of hepatitis type B among the other causes of hepatitis occurring in thecommunity. A total of 762 individuals were included in the study from whom blood samples were collected. Theoverall vaccination coverage among the study population was 62.9% leaving 37.1% unvaccinated. An enzymelinkedImmunosorbent assay (ELISA) was used for the determination of anti-HBs-IgG antibody, showed aprevalence of 71.2%, 66.7%, 51.4%, and 8.7% for barbers, medical personnel (occupational exposure), childrenunder 15years of age and unvaccinated adults respectively. There was a clear effect for the number of vaccine dosesadministered on the levels of seroconversion, with no significant differences in antibody associated with sex orgeographical distribution of vaccinees, but there was a difference in relation to occupation. There was a significantdecline in the levels of antibody overtime post vaccination and the losses of protective levels of antibodies were quiteevident by 3-4 years post the primary vaccine doses which stress the need for booster doses. In the primaryvaccination schedule the time interval of 5-6 months between the 2nd and 3rd vaccine doses is suitable for betterresponses to HBV vaccine. The proportion of icteric HB was 14.7% while 85.3% was due to other hepatitis causes,and the rate of hepatitis B among individuals with no history of hepatitis was 2.6% referring to the subclinical cases.

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Article
DIAGNOSTIC VALUE OF ANTI-PEPTIDYLARGININE DEIMINASE TYPE 4 (PADI-4) AND ANTI- CITRULLINATED PEPTIDE ANTIBODIES (ACCP) IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS

Authors: Hassan J Hasony --- Hisham Abd-AlJalel Badran
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2014 Volume: 20 Issue: 1 Pages: 39-46
Publisher: Basrah University جامعة البصرة

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This study aimed to examine the value of anti peptidylarginine deiminase (anti-PADI-4) antibody and anti-cyclic citrullinated peptide (anti-CCP) antibody among Iraqi patients with RA and to determine whether the activity and severity in RA patients are associated with anti-PADI- 4 and anti-CCP antibodies positivity. In a case control study, we determined the seropositivity of these two serological markers anti- PADI-4 and anti-CCP antibodies in100 RA patients and 100 healthy controls subject. Activity of disease was measured by disease activity score in 28 joints (DAS 28) and severity was assessed using Scott modification of the Larsen method. Antibodies to PADI-4 were detected in (32%) among RA patients and present (1%) in controls (p < 0.001). Anti-CCP was present in (74%) of the RA patients and present in 2% in controls (p < 0.001), sensitivity was highest for anti-CCP antibody (74%) followed by anti-PADI-4 antibody (32%). Specificity was highest for anti-PADI-4 antibody (99%) followed by anti-CCP antibody (98%). A significant correlation with disease activity was observed in both markers, RA patients on remission had negative PADI-4 test (0/12) but patients with high disease activity showed higher percentage (86.4%) of PADI-4 seropositivity in compared to anti-CCP where patients on remission had (8.3%) seropositivity and RA patients with high disease activity had (91%) anti- CCP seropositivity. However, seropostivity to PADI-4 was significant correlated with RA severity (p < 0.001) as evaluated by scott grade (59.3%) in grade 5. Similarly, there were a significant correlation between anti-CCP seropositivity and radiological finding but less than that associated with seropositivity to PADI-4 antibody (p < 0.05). In conclusion, with their excellent specificity, both anti-PADI-4 and anti-CCP antibodies can be useful in establishing the diagnosis of RA, also both markers are good predictors of disease activity and severity.


Article
Enteric adenovirus associated with acute gastroenteritis among hospitalized and healthy childern under five - years of age in Basrah, Iraq
الفيروس الغدي المعوي المرتبط بالتهاب المعدة والأمعاء الحاد بين الأطفال في المستشفى والأصحاء دون الخامسة من العمر في البصرة، العراق

Author: Hazim T . Thewainy, Hassan J. Hasony
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2019 Volume: 37 Issue: 1 Pages: 37-44
Publisher: Basrah University جامعة البصرة

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Background: Enteric Adenovirus are second to rotavirus as viral cause of acute gastroenteritis in infants and young children. Aims of the study: This study aimed on determination of the incidence of enteric adenovirus-associated gastroenteritis in infants and young children below five years of age, and to detect the season and age related distribution of enteric adenovirus infections as well as the major clinical symptoms associated with these virus infections.Objective: A total of 400 stool specimens (200 symptomatic diarrheal cases and 200 asymptomatic normal children) were collected during the period from March 2011-March 2012. All relevant informations were obtained on special questionnaire form. Viral genomic DNA was extracted from stool specimens by using a spin column technique according to the instructions given by QIAamp-MinElute virus spin kit for purification of virus genome (Qiagen, Germany). Results: Enteric adenovirus was detected by the use of specific primers. Enteric Adenovirus was detected in 3% of diarrheal cases in hospitalized children whereas all healthy children were negative for enteric adenovirus. Age group analysis revealed that children at age groups of 9-11 and 12-17 months were more affected. The monthly distribution of enteric adenovirus cases showed to be confined to a period of 4 months (August through November). The clinical symptoms associated with adenovirus gastroenteritis was dehydration (80%), vomiting (60%) and fever (60%) while abdominal pain was not recorded.Conclusion: Enteric adenovirus is common enteric pathogens as that for rotavirus in our community.

خلفية الدراسة: الفيروس الغدي المعوي هو الثاني لفيروس الروتا كسبب فيروسي لالتهاب المعدة والأمعاء الحاد عند الرضع والأطفال الصغار. أهداف الدراسة: هدفت هذه الدراسة إلى تحديد مدى حدوث التهاب المعدة والأمعاء المرتبط بفيروس الغدي المعوي عند الرضع والأطفال الصغار دون الخامسة من العمر، واكتشاف التوزيع المرتبط بالسن والتوزيع المرتبط بالعمر بالإضافة إلى الأعراض السريرية الرئيسية المرتبطة لهذه الالتهابات الفيروسية.الطريقة: تم جمع ما مجموعه 400 عينة من البراز (200 حالة من حالات الإسهال و 200 من الأطفال الطبيعيين بدون أعراض) خلال الفترة من مارس 2011 إلى مارس 2012. تم الحصول على جميع المعلومات ذات الصلة في نموذج استبيان خاص. تم استخراج الحمض النووي الجينومي الفيروسي من عينات البراز باستخدام العدة الخاصة التشخيصية من QIAamp-MinElute لتنقية جينوم الفيروسات (Qiagen، ألمانيا). تم الكشف عن الفيروس الغدي المعوي عن طريق البلمرة المتسلسلة (PCR).النتائج: تم الكشف عن الفيروس الغدي المعوي المعدي في 3٪ من حالات الإسهال في الأطفال في المستشفى في حين أن جميع الأطفال الأصحاء كانوا سلبيين الفيروس الغدي المعوي. وكشف تحليل الفئة العمرية أن الأطفال في الفئات العمرية (9-11و12-17شهرا كانوا أكثر تضررا. الاستنتاج: أظهر التوزيع الفصلي لحالات الفيروس الغدي المعوي أن يقتصر على فترة 4 أشهر (أغسطس وحتى نوفمبر). كانت الأعراض السريرية المرتبطة بالتهاب المعدة والأمعاء في الفيروس الغدي هي الجفاف (80٪) والقيء (60٪) والحمى (60٪) في حين لم يتم تسجيل آلام في البطن.


Article
4- THE ASSOCIATION OF HUMANA PAPILLOMAVIRUS WITH CERVICAL NEOPLASM IN BASRAH

Authors: Hassan J Hasony --- Saad Abdulbaqi --- Rafan Oday Fahad
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2011 Volume: 17 Issue: 1 Pages: 14
Publisher: Basrah University جامعة البصرة

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Original ArticlesRafan Oday Fahad*, Saad Abdulbaqi# & Hassan J Hasony@*MBChB, MSc Microbiology. #MBChB, CABP, Department of Pathology. @ MPhil, PhD, Department ofMicrobiology, College of Medicine, University of Basrah, Basrah, Iraq.AbstractCervical cytobrush and Pap smears were collected from 103 women attending the outpatientdepartment at Basrah Maternity and children hospital during the period from October 2009 tillthe end of January 2010. DNA was successfully extracted from 91 cytobrush samples, amplifiedfor the detection of human papilloma viruses (HPVs) using GP5+/GP6+ primers, in addition totyping using type-specific primers for HPV-16 and HPV-18 genotypes.The overall HPV prevalence was 20.8% with the dominance of genotype 16 (36.6%) over thegenotype 18 (10.5%) and the presence of non-16, non-18 genotype(s) in 42.1% of all HPVpositive cases. Younger women aged 25 years or less were more infected (26.3%) with thedominance of genotype-16 (21.1%) and among women aged 36-45 years, the non-16/non-18genotype(s) were the more frequently observed (13.3%). Infection rates were more frequentamong women married above 30 years (33.3%) and those with more than one lifetime husband(28.6%). Women's husband with polygamy practice significantly covariate with HPV infections(P< 0.05).Significant association (P<0.001) was obvious between infection with any HPV and abnormalcytology. The non-16/non-18 genotype(s) were more involved (28%) whereas genotype-16 wasdetected more frequent (20%) than genotype-18 (4%). In addition mixed infection of both highrisk types (16 and 18) was limited only to women with abnormal Pap smears.In archival blocks, the presence of HPV- DNA was constant in pre-invasive sequamous cervicallesions as all blocks revealed a positive HPV-16 either alone or in combination with genotype-18.

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Article
10- HUMAN PARVOVIRUS B19 ANTIBODY AMONG ARTHROPATHIC PATIENTS WITH ESPECIAL EMPHASIS ON SICKLE CELL DISEASES IN BASRAH

Authors: MUBDER A MOHAMMED SAEED --- HASSAN J HASONY --- DANIAH M SHAKIR
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2008 Volume: 14 Issue: 2 Pages: 53
Publisher: Basrah University جامعة البصرة

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A case control study was carried out during the period from October 2006 till September 2007in Basrah Governorate. To estimate the overall prevalence of Human Parvovirus B19 (HPVB19) antibody and its association to Rheumatoid factor seropositivity among sickler and nonsickler arthropathic patients.A total of 182 blood samples were collected. Ninety (90) from arthropathic patients with orwithout sickle cell diseases (SCD), who attended the orthopaedic, rheumatology and internalmedicine consultant clinics in Basrah General Hospital and Hemoglobinopathies Center at theMaternity and Children Hospital. Ninety two (92) from control group, non arthropathic with orwithout SCD.Human parvovirus B19 IgG antibodies were serologically detected by an Enzyme- LinkedImmunosorbent Assay (ELISA). The rheumatoid factor was detected by Latex agglutination test.The overall prevalence of HPV B19 antibody in Basrah among study population was 68.7% .The prevalence of this antibody was 76.9% among arthropathic non sickler and 65.8% in sicklerarthropathic patients. These differences were shown statistically not significant P> 0.05compared to control group where prevalence was 63.2% in non arthropathic sickler and 66.7%among non arthropathic non sickler individuals.Eighty percent of arthropathic patients who had positive rheumatoid factor were also positive forHPV B19 antibody, which indicate a significant association (P<0.05).In the present study the seropositivity of HPV B19 was shown to be increased with age. Inrelation to site of joint affected, the small joints of the hand and foot were the commonest site ofmanifestation 78.1%.HPV B19 antibody was significantly more (79.7%) among persons with history of bloodtransfusion. The types of SCD had no significant effect on the prevalence of HPV B19 antibody(P>0.05). However there was a positive relation between HPV B19 seropositivity and theduration of illness (P<0.0%).In conclusion, HPV B19 is common with high prevalence in our region. There is clearassociation between HPV B19 infection and rheumatoid factor positivity. Individuals with sicklecell diseases regardless the type and those with history of blood transfusion were considered asrisk groups for acquiring HPV B19 infections.

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Article
The Clinical Significance of Interleukin-15 and Interleukin -17 in Patients with Rheumatoid Arthritis

Authors: Noora Z Muhammed --- Hassan J Hasony --- Mubder A Mohamed Saeed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 4 Pages: 560-570
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND: Rheumatoid arthritis (RA) is a chronic, deforming arthritis that can lead to disabilities and poor quality of life. Cytokines produced by inflammatory cells play a pivotal role in synovial inflammation and joint destruction in rheumatoid arthritis; they are the final mediators and/or regulators of inflammatory process.OBJECTIVE: This study was designed to assess serum IL-15 and IL-17 levels in a sample of patients with RA and to evaluate the correlation between their levels and various clinical, laboratory parameters of RA disease activity and severity.SUBJECTS AND METHODS: A case – control study was carried out from January 2013 till October 2013. It was conducted on 80 RA patients who attended Basra General Hospital outpatient rheumatology clinic. Eighty apparently healthy adults, age and sex matched, were also included as control group. Peripheral blood samples were collected from both patients and controls and used for estimation of serum IL-15, IL-17 and anti-CCP antibodies by ELISA, RF and CRP by slide agglutination test and ESR by Westergren method. RA activity was measured using disease activity score28 (DAS-28).RESULTS: RA patients showed significantly higher serum IL-15 and IL-17 levels than controls (P<0.01). In addition, IL-15 and IL-17 were found to be significantly higher in RA patients with active disease compared to those at remission. No significant correlations were noted between IL-15 serum level and RF, CRP, ESR, anti-CCP but it was significantly correlated with DAS-28, TJC and SJC. Significant positive correlations of serum IL-17 level and ESR, CRP, anti-CCP, DAS28, TJC and SJC were found. Also there was a significant direct correlation between IL-15 and IL-17concentration.CONCLUSION: The current study demonstrated that serum IL-15 and IL-17 levels were significantly higher in RA patients than the controls confirming their important role in the pathogenesis of RA and possible target for future therapy .Ele¬vated IL-17 levels could be considered as a possible indicator of more severe clinical course, This is underscored by relatively strong correlations between almost validated disease activity and severity markers including CRP, ESR, anti-CCP, DAS28, TJC and SJC.


Article
THE ASSOCIATION OF AGE AND NUTRITIONAL STATUS WITH SEROCOVERSIOIN TO ORAL POLIOVIRUS VACCINE (OPV) IN BASRAH, IRAQ
علاقة العمر والحاله التغذويه بالا ستجابه ألمنا عيه للقاح شلل الأطفال بالبصرة

Authors: Basam D. Salman --- Hassan J Hasony د.بسام سلمان* , أ.د. حسن جابر حسوني**
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2005 Volume: 23 Issue: 1 Pages: 47-52
Publisher: Basrah University جامعة البصرة

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ABSTRACTA seroepidemiological study was carried out in Basrah, southern Iraq from November 1997 until the end ofApril 1998. Blood samples were collected from 3 sources; Basrah Maternity and Child Hospital, the primaryhealth care centers in Al-Zubair and Abu-AlKhasib. The relevant information’s were obtained from mothersthrough interview and/or vaccination cards. An Enzyme linked Immunosorbent assay (ELISA) was used forthe detection of poliovirus IgG antibodies. Poliovirus antibodies were found in 92.85% with almost fullseroconversion among children above 2 years of age, while seronegative cases were present among infant’schildren below the age of two years and those with old vaccination history and not boosted with additionalbooster doses. Malnourished children have relatively lower levels of antibody titer than well nourished children.Number of vaccine doses given to the child have a significant effect on antibody prevalence where high levelsof immunity were found in children who received 5 doses or more while seronegative cases were detectedamong children who had been given 4 doses or less, There were relatively higher seropositivity amongchildren who received an extra doses during the National Immunization Days (NID) than among thosewho did not receive extra dose(s).The antibody levels in the blood is partially affected by age and the numberof vaccine doses given to the child where the proportion of samples with low antibody levels decreased as theage and number of vaccine doses increases. There was clear evidence for the overtime antibody losses among theunboosted children.

أجريت دراسة مصليه وبائية للفترة من شهر تشرين الثاني 1997 حتى نهاية شهر نيسان 1998 على عينات دم جمعت من أطفال من فئات عمريه مختلفة ملقحين من ثلاثه مراكز في البصرة هي : مستشفى البصرة للولادة والطفل ومركزي الرعاية الصحية الاوليه في الزبير وأبي الخصيب واستحصلت المعلومات اللازمه من خلال استبيان خاص أساسه مقابلة الأمهات والمعلومات المثبته في بطاقة التلقيحات الخاصه بكل طفل وقد اعتمدت طريقة المعايره المناعيه الانزيمي ELISA للتحري عن المضادات IgG لحمات شلل الاطفال علـى مصل الدم من 308 طفل أشركوا في الدراسه من فئات عمريه مختلفه تحت جرعات لقاح مختلفه وبفترات مختلفه حسب تصميم الدراسه.من تحليل النتائج وجدت مضادات حمات شلل الاطفال عند 85ر92% عند الفئات العمريه الاكثر من سنتين بينما كانت العينات السالبه عند الفئات العمريه دون السنتين والذين لقحوا منذ فترات بعيده ولم يأخذوا الجرعات السانده من اللقاح، كانت معدلات التمنيع دون المستـوى المطلوب عند ألاطفال الذين يعانون من سوء التغذيه مقارنه بالاصحاء عند الفئات العمريه المتناظره. وبينت الدراسة ان لعدد الجرعات اللقاحيه السانده بعد الثلاثيه الاوليه تأثير كبير على استمراريـــة مستويات معتده من المناعيه الوافيه وان المستويات المناعيه المتدنيه تزداد بزيادة العمر مما يوصي بوجــود تعرض طبيعي لحمات شلل الاطفال .كذلك فان هناك فقدان بالمستويات الواقيه للمناعه مع مرور الزمـن العامل الذين يوجب الاستمرار بالحملات الدوريه لاعطاء الجرعات السانده من اللقاح.

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Article
THE ASSOCIATION OF ACUTE HUMAN PARVOVIRUS B19 INFECTION AND SPONTANEOUS MISCARRIAGE IN BASRAH, IRAQ
العلاقة بين فقدان الحمل المتكرر عند النساء الحوامل مع اخماج حمات البارفو- ب19 في البصرة

Authors: SADOON R N --- HASSAN J HASONY أ.د. حسن جابر حسوني1، د. رقية نوري سعدون2
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2011 Volume: 29 Issue: 1&2 Pages: 19-25
Publisher: Basrah University جامعة البصرة

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ABSTRACTA case control study was carried out during October 2008 till September 2009 in Basrah. The study was designed to evaluate the occurrence of acute human parvovirus B19 infection among pregnant women and its association with spontaneous abortion.A total of 182 blood samples were collected from the outpatient clinic, wards and emergency unit at Basrah Maternity and Children Hospital, 91 from women with spontaneous abortion and 91 from women with normal pregnancy as control matching cases in age and gravidity. Maternal serum was kept frozen at –20ºC until serological investigation by Enzyme-linked immunosorbent assay to detect parvovirus B19 IgM (ELISA-B19-IgM), then the positive cases were investigated to determine the level of interferon-gamma by ELISA kit and acute phase protein C-reactive protein (CRP) by latex agglutination test.The overall prevalence of parvovirus B19 in study population was 53.8% for cases and 41.8% for control. All B19 positive cases were positive for interferon-gamma in different titers and 77.6% of B19-IgM positive cases were positive to CRP as well. The data indicate that the diagnosis of B19 infection during pregnancy should be considered more often, particularly in complete and missed types of abortion, as missed abortion represents more than 50% of B19 seropositive of spontaneously aborted ladies. Significantly (P<0.05) high percentage of B19 positive cases as well as fetal losses were observed in the second trimester. Also the results showed significant difference in B19 antibodies in relation to gravidity (P<0.05).The main conclusion that human parvovirus B19 is common with high prevalence rates among pregnant in our region, and cases of complete and missed abortion should be investigated to exclude parvovirus B19 infections.

فرع الأحياء المجهرية / كلية الطب / جامعة البصرةأجريت دراسة منضبطة على 182 عينة دم أخذت من 91 امرأة حامل تعاني من فقدان الحمل المتكرر و 91 امرأة حملهن طبيعي كعينة ضابطة خلال الفترة من تشرين الأول 2008حتى أيلول 2009 لتقييم العلاقة بين فقدان الحمل المتكرر والإصابة باخماج حمات البارفو بـ19 عند النساء الحوامل. جمعت العينات من مستشفى البصرة للأطفال والولادة وقد حفظت المصول المعزولة تحت درجة انجماد – 20 درجة مئوية وتم التحري عن أضداد حمات البارفو – ب19- بواسطة المقايسة المناعية المرتبطة بالإنزيم ثم فحصت العينات الايجابية للتحري عن وجود الانترفيرون –كاما بواسطة المقايسة المناعية المرتبطة بالأنزيم وقياس مستويات البروتين القياسي لوجود الالتهابات الحادة بواسطة فحص اللاتكس.ELISA لقد كان وجود أضداد البارفو فايرس – ب-19 عند عينة الفحص 8و52% و 8 و41% عند العينات الضابطة كذلك وجد ان جميع عينات الفحص (100%) موجبة لوجود انترفيرون – كاما و6و77% منها. تشير نتائج البحث الى ان عند حدوث الإسقاط من النوع الكامل ومن النوع غير المحدد انCRP لفحص يأخذ بنظر الاعتبار ان هذه الفيروسات قد تكون إحدى المسببات وان نسبة يعتد بها جدا من المريضات قد يفقدن حملهن عند الربع الثاني من الحمل وان هناك علاقة بين الإصابة بالفيروس وفقدان الحمل. وبشكل عام فان حمات البارفو – ب19 موجودة بشكل كبير ويجب احتمال وجودها كمسبب في العديد من حالات فقدان الحمل. المجلة الطبية لجامعة البصرة ص19-25

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Article
7- DIAGNOSTIC VALUE OF ANTIBODIES TO CITRULLINE CONTAINING PEPTIDES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTHERN IRAQ43

Authors: & THURAYA J MEZAL --- ADNAN T AL-KHAFAJI --- HASSAN J HASONY --- ALI M BARAKAT
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2009 Volume: 15 Issue: 1 Pages: 43-50
Publisher: Basrah University جامعة البصرة

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A serological study has been done in Al-Nassiriyha, southern Iraq, from December 2006 toAugust 2007 designed to determine the frequency of anti-cyclic citrullinated peptide antibody(anti-CCP) in a group of patients with rheumatoid arthritis for specific period of time comparedwith other rheumatic diseases and healthy controls. Also to show the diagnostic value ofanti-CCP antibody in relation to other markers [rheumatoid factor (RF), antinuclear factor (ANF),C-reactive protein (CRP) and cytokines such as interleukin–6 (IL-6), tumor necrosis factor–α(TNF-α)] to discriminate between those patients with and without RA.Blood samples were taken from 121 patients with rheumatoid arthritis, 88 patients of otherrheumatic diseases from AL-Hussein Teaching Hospital in AL-Nassiriyah and 120 healthycontrols from medical personnel and school students who were healthy. All the information wastaken from the patients and controls.An Enzyme-linked immunosorbent assay was used for estimation of anti-CCP, IL-6, TNF-α andslide agglutination test for the estimation of RF, CRP and ANF. The seropositivity to anti-CCP inRA patients group was 61.2% among them 23% with low levels of anti-CCP, 25% withmoderate levels and 51.3% with high levels (> 60 units /ml.).The high levels of anti-CCP persistfor long durations up to 10 years (54.7%). However, anti-CCP was rarely detected among non-RA rheumatic diseases and healthy controls (2.3% and 2.5% respectively). Anti-CCP showedthe greater specificity (97.6%) and sensitivity (61.2%) compared to the other marker used in thisstudy. The overall agreement between the tested parameters in their ability to detect RA patientwas higher for anti-CCP combined to IL-6 (91.7%) in comparison to the effect of other markerscombination. Although anti-CCP was more common in RA patient with 2-10 years duration ofillness, anti-CCP, IL-6 and TNF-α positivity as estimated by ELISA were persistently with hightiters regardless the duration of illness. The majority of RA patients with high anti-CCP levelswere among stage three of clinical RA. Smoking has a positive relation to the increased levelsof all tested parameters compared to the non-smokers.

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RHEUMATOID --- ARTHRITIS


Article
Detection of Norovirus in stool samples by RT-PCR from under five years age children hospitalized for acute gastroenteritis in Basrah, Iraq
الكشف عن فيروس النورو في عينات البراز بواسطة تقنية تفاعل البلمرة المتسلسل العكسي في الاطفال الاقل من خمسة سنوات من العمر الراقدين في المستشفيات والذين يعانون من التهاب المعدة والامعاء الحاد في البصرة، العراق

Authors: Hazim T. Thwiny حازم طالب ثويني --- Hassan J. Hasony حسن جابر حسوني
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2015 Volume: 5 Issue: 2 Pages: 47-53
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Human noroviruses are the leading cause of non-bacterial gastroenteritis in children worldwide. The objectives of this study are to determine the frequency and importance of norovirus in diarrheal illness among children below 5 years of age and to investigate the most affected age, seasonal distribution and the major clinical symptoms associated with norovirus infections; 400 stool samples (200 symptomatic cases and 200 asymptomatic healthy children) were investigated during the period from March 2011 to March 2012.Norovirus was detected in 8% of symptomatic children whereas all the control groups were norovirus negative. Norovirus was highly prevalent among children of 18-23 months of age (P=0.05). Noroviruses were continuously detected throughout the year except in May and October, but the principle peaks of detection were in November. The most common clinical symptoms beside diarrhea observed among norovirus infected children were vomiting (94%), fever (69%), dehydration (50%) and abdominal pain found in 31%.

تعتبر فيروسات النورو الخاصة بالانسان مسببا رئيسيا لالتهاب المعدة والامعاء الحاد الغير بكتيرية على مستوى العالم. اهداف هذه الدراسة تحديد اهمية وتكرار فيروس النورو بين الاطفال المصابين بحالات اسهال وتقل اعمارهم عن خمسة سنوات, لتحديد العمر الاكثر تعرضا للمرض, الانتشار الموسمي للمرض والعلامات الرئيسية المصاحبة للاصابة. تم فحص 400 عينة من البراز (200 من الحالات المرضية و 200 من الحالات الغير مرضية) خلال الفترة من اذار 2011 الى اذار 2012. كانت نسبة وجود الفيروس هي 8% في الاطفال الذين تظهر عليهم علامات سريرية, بينما في مجموعة السيطرة لم يتم الكشف عن الفيروس فيها. الفيروس كان اكثر شيوعا في الاطفال بعمر 18-23 شهر (P=0.05). اما بالنسبة للانتشار الموسمي للمرض, فقد تم الكشف عن الفيروس في كل اشهر السنة ما عدا شهري ايار وتشرين الاول وان الاصابات الاكثر كانت في شهر تشرين الثاني. اهم الاعراض السريرية بجانب الاسهال بالاطفال المصابين بالفيروس كان التقئ بنسبة 94%, الحمى بنسبة 69%, الجفاف بنسبة 50%, والم في البطن بنسبة 13%.

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