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Identification of Streptococcus mutans from Human Dental Plaque and Dental Caries Using 16SrRNA Gene
تشخيص بكتريا Streptococcus mutans من عينات تسوس الاسنان واللعاب باستخدام جين 16SrRNA

Authors: Adhraa S. Flayyih عذراء صالح فليح --- Hayfa H. Hassani هيفاء هادي حساني --- Mohammed H. Wali محمد حسين والي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 1C Pages: 552-557
Publisher: Baghdad University جامعة بغداد

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Abstract

One hundred and sixty samples from saliva and dental plaque were sellected from patients with caries active at ages from (4-65) years from Umm Qasr Primary School and Al-Ameria Specialist Dental Center in Baghdad. 15 isolates belong to Streptococcus mutans were identified by biochemical tests and Vitek 2 compact system while 22 isolates identified by using Polymerase Chain Reaction ﴾PCR﴿ techniques and sequencing of 16SrRNA with 120 bp by using 16SrRNA the result confermed that these isolates were belong to S.mutans

جمعت مائة وستون عينة من اللعاب و الاسنان المتسوسة من مرضى يعانون من تسوس الاسنان باعمارمختلفة تراوحت بين (4-65) سنة عند مراجعتهم مركز العامرية التخصصي لطب الاسنان ومن طلبة مدرسة ام قصر الابتدائية , شخصت خمسة عشر عزلة بانها S. mutans بالاختبارات الكيمو حيوية ونظام الفايتك -2 بينما 22 عزلة شخصت باستخدام تقنية سلسلة تفاعل البلمرة الPCR وجين 16SrRNA بحجم120 زوج قاعدة واثبتت النتائج ان هذه العزلات تعود الى S.mutans.


Article
Cytotoxic effect of Azurin Produced from Pseudomonas aeruginosa Ps21 on cancer cell lines (Hep-2 and AMN-3)
التأثير السمي للأزورين المنتج من بكترياpseudomonas aeruginosa PS 21 في الخطوط الخلوية السرطانية Hep-3 و 2 AMN-

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Abstract

This stady was amied to detection the ability of azurin , produced from Pseudomonas aeruginosa Ps21, as inhibitortwo types of cancer cell lines, human laryux epidermoid carcinoma ( Hep – 2 ) and Mouse Mammary adenocarcinoma(AMN – 3 ) by using cytotoxicity assay.The Results showed the effect of partial purified of azurin depended on the concentration , type of cell and the exposuretime. The extract at high concentrations ( 125 , 250 ) μg/ml. were exhibited apotent inhibiory at all exposure timeagainst cancer cell lines. While The inhibitory activity was gradually reduced with decreasing of concentration ofazurin , the low concentrations were activated for the cells proliferation in comparison with the control (100 %).The viability of Hep – 2 cells was increased only during the first 24 hrfrom exposure while the AMN – 3 cells were proliferated at all exposture times (24 , 48 , 72) hr.

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