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Article
Detection viral load of Parvovirus B19 in patients with Chronic Renal Failure

Authors: Sattar G. Ajeel --- Huda D. hathal --- Jawad K. mnate
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 3 Pages: 40-44
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background : Because of Parvovirus B19 that occur in patients with chronic renal failure ; Parvovirus B19 was found to be associated with or might be an aggravation factor of renal failure , we tried to find out the rate of occurrence of this virus in dialysis and non dialysis patients. Objective: The aim of the study was to investigate viral load for Parvovirus B19 was play an important role in the etiology of chronic renal failure patients Subjects and methods :A cross sectional study included Serum samples were collected from 50 dialysis patients and 50 without dialysis patients 50 normal subjects as control, and subjected for ELISA technique for detection of parvovirus IgG antibody, and multiplex Real time PCR for detection virus and viral load of this virus Results: Thirteen (26%) out of these 50 dialysis patients were positive by RT-PCR and, 16% (8/50) were positive by ELISA, while none of the controls was positive neither by RT-PCR nor by ELISA. The results of this study showed highly significant differences (p<0.001) on comparing between the median viral load in patients who had dialysis (2.38x106) copies / ml of Parvovirus B19 DNA and those who without dialysis(1.73x104) copies/ml of Parvovirus B19 DNA. Also the results of B19 by real time PCR analysis showed a significant difference (p<0.001) in median viral load in patients who had acute infection(2.87x106) copies/ml of Parvovirus B19 DNA ,than those who had chronic infection (2.29x105) copies/ml of Parvovirus B19 DNA , Conclusions Parvovirus B19 could be an important co-factor that play a role in CKD, which was higher rate in dialysis patients.


Article
5.DETECTION OF PARVOVIRUS B19 IN BAD OBSTETRIC HISTORY BY USING REAL TIME PCR

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Abstract

Background: Human parvovirus B19 (B19V) is a small single-stranded DNA virus. Infection during pregnancy can cause a variety of signs of fetal damage. The risk of adverse fetal outcome is increased if maternal infection occurs during the first two trimesters of pregnancy but may also happen during the third trimester.Objective: to determine the screen of parvovirus B19 in pregnant women with bad obstetric history by real time polymerase chain reaction (PCR).Methods: Two hundred Plasma and 200 placental tissue samples were collected from all pregnant women enrolled in this study. Three ml whole blood was collected in sterile EDTA-blood tube. Plasma was obtained by centrifugation of whole blood. Twenty-five grams of the placental tissue was homogenized with 10 ml of PBS by using tissue homogenizer for about 1 min at 4 °C. Results: 40 (20%) out of 200 plasma samples were real time PCR positive, the remainder 160 (80%) were real time PCR negative. Nineteen (9.5%) out of 200 placental tissue samples were positive for B19 real time PCR the remainder 181 (90.5 %) were real time PCR negative. All placental tissue positive (n=19) were positive by real- time PCR in plasma samples (n=40). Out of 40 pregnant women presented with positive parvoviruses results in current study demonstrated 21 (52.5%) gave abortion in first trimester and only 8 (20%) gave abortion in second trimester.Conclusion: Parvovirus B19 is common and highly distributed among pregnant ladies in this study and there is a significant association between B19 positivity and adverse pregnancy outcome.Keywords: Parvovirus B19, bad obstetric history, adverse pregnancy outcome, non-immune hydrops fetalisCitation: Abdulhassan LF, Hathal HD, Abdullah TH. Detection of Parvovirus B19 in bad obstetric history by using Real Time PCR. Iraqi JMS. 2017; Vol. 15(4): 350-357. doi: 10.22578/IJMS.15.4.5


Article
7.PREVALENCE AND DIAGNOSIS OF SEXUALLY TRANSMITTED PATHOGENS IN A SAMPLE OF IRAQI WOMEN: A MOLECULAR STUDY

Authors: Mariam K. Ali مريم كريم علي --- Huda D. Hathal هدى ظاهر هذال --- Hala A. Almoayed هالة عبد القادر المؤيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 4 Pages: 364-376
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Sexually transmitted infections (STI), also referred to as sexually transmitted diseases (STD) and venereal diseases (VD), are infections that are commonly spread by sex, especially vaginal intercourse, anal sex and oral sex. Most STIs initially do not cause symptoms. This results in a greater risk of passing the disease on to others. Symptoms and signs of disease may include: vaginal discharge, penile discharge, ulcers on or around the genitals, and pelvic pain.Objective: To detect the two microorganisms (Gardnerella vaginitis (G. vaginalis) and Trichomonas vaginalis (T. vaginalis)) in the same sample taken from women with genital tract infection by microbiological and molecular methods and to investigate the contributions of some socioeconomic factors and clinical features.Methods: Two hundred samples were collected from females attending the Gynecology out-patient department in the Al-Imamein Al-Kadhimein Medical City and Baghdad Teaching Hospital during the period from May 2014 to April 2015. Based on availability of full clinical information about each patient, high vaginal swabs were taken from females at different ages (15-54 years) representing patients group complaining of abnormal vaginal discharge with or without other symptoms, questionnaire was applied. The two diseases associated with vaginal infection include G. vaginalis and T. vaginalis. Each of the vaginal swabs collected was examined microscopically, whilst the remaining was preserved at -20 °C for DNA extracts were analyzed with the real-time poly meres chain reaction (RT-PCR). Results: In RT-PCR, the rate of infection was 120 (60%) G. vaginalis, and 34 (17%) T. vaginalis. Highest rate of infection in women with G. vaginalis was among age group (15-24) years and (25-34) years 38.3%, 35.0% respectively, the lowest rate was among age group (45-54) years 8.3 %. In T. vaginalis, the highest rate of infection was among age group (15-24) years 61.7 %, the lowest was among age group (35-44) years 8.8 % and on infection in age 45-54years.Conclusion: The commonest genital tract infections among women were G. vaginalis and T. vaginalis. Molecular methods are considered the gold standard for diagnosis, given the excellent sensitivities and specificities in diagnosis. Presence of clinical symptoms helps and lab diagnosis of infection. Vaginal swab samples showed that most common co-infection is between G. vaginalis cases and T. vaginalis.Keywords: Sexually transmitted infections, Gardnerella vaginalis, Trichomonas vaginalis, molecular studyCitation: Ali MK, Hathal HD, Almoayed HA. Prevalence and Diagnosis of sexually Transmitted Pathogens in A Sample of Iraqi Women: A Molecular Study. Iraqi JMS. 2017; Vol. 15(4): 364-376. doi: 10.22578/IJMS.15.4.7


Article
2.PREVALENCE AND DIAGNOSIS OF GENITAL HERPES BY IMMUNOLOGICAL AND MOLECULAR STUDY

Authors: Hala A. Almoayed هالة عبد القادر المؤيد --- Huda D. Hathal هدى ظاهر هذال --- Mariam K. Ali مريم كريم علي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 1 Pages: 4-7
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Genital herpes simplex infection is a viral infection caused by the herpes simplex virus (HSV) type 1 or 2. This disease transmitted during close skin or mucus membranes contact with an infected person who is shedding the virus. Infections that are commonly spread by sex, especially vaginal intercourse, anal sex and oral sex.Objective: For detection of HSV by immunological and molecular methods.Methods:Two hundred (200) samples were collected from females attending the Gynecology Outpatient Department in the Al-Imamein Al-Kadhimein Medical City and Baghdad Teaching Hospital during the period from May 2014 to April 2015. Based on availability of full clinical information about each patient, high vaginal swabs were taken from females at different ages (15-54 years) representing patients group complaining of abnormal vaginal discharge with or without other symptoms. The Statistical Analysis System- SAS program was used to study the effect of difference factors in study parameters. Chi-square test was used to significant comparison between percentages in this study. Results: Each of the vaginal swabs collected were examined, was preserved at -20 °C for DNA extracts were analyzed. In RT-PCR, the rate of infection was in women with HSV, those with age group (25-34) years and (35-44) years were 50%.Conclusion: HSV infections were detected in genital tract infection in women; molecular methods are considered the gold standard for diagnosis, given the excellent sensitivities, specificities, rapid and accurate laboratory diagnosis of HSV.Keywords: Genital herpes, diagnosis, immunological, molecular studyCitation: Ali MK, Hathal HD, Almoayed HA. Prevalence and diagnosis of genital herpes by immunological and molecular study. Iraqi JMS. 2018; Vol. 16(1): 4-7. doi: 10.22578/IJMS.16.1.2

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