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Article
Herbal activation of mammary gland; a comparative

Authors: Fadhil A Al-Khafaji --- Huda M Al-Khateeb* --- Malak A Al-Yawer
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 1 Pages: 144-146
Publisher: Baghdad University جامعة بغداد

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Abstract

Seventy five adult virgin female Norway rats (60 experimental and 15 controls) were used toevaluate the effect of seeds of three herbs (Fennel, Cumin and Garden cress) on their mammaryglands. Experimental animals were fed with these herbs (each type of herb seeds was given to twentyexperimental rats) for fourteen days. Rats were sacrificed and mammary gland sections wereobtained, stained then morphometrically assessed. Serum prolactin level was performed too.Results revealed that Garden cress seeds are the strongest lactogenic agent among the three. BothFennel and Cumin seeds were shown to be very weak galactagogues.


Article
Garden Cress Seed Could be A Factual Galactagogue

Authors: Malak A Al-Yawer --- Huda M Al-Khateeb --- Fadhil A Al-Khafaji
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 1 Pages: 62-67
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: Trivial number of books, concerning traditional medicine, had mentioned a galactagogual role of Garden cress seeds. Others ignore that. This controversy, in addition to the steroid (family of sex hormones) contents of the seeds, directed us to evaluate the role of this herb in mammogenesis and lactogenesis. METHODS: Twelve parameters were used to assess the effect of Garden cress seeds on the mammary gland of young adult virgin rats. These parameters comprise gross assessment, histological examination (routine/ haematoxylin and eosin stain and special stain/ PAS), enzymatic histochemical study (alkaline phosphatase, acid phosphatase and lipoprotein lipase cytochemical localization), biochemical estimations (hormonal assay of FSH, LH, prolactin, estrogen and progesterone) and morphometrical measurements. RESULTS: All parameters significantly exhibited a strong mammotrophic and lactogenic effects of Garden cress seeds on the non-primed mammary gland of adult virgin rat. CONCLUSION: Garden cress seeds are most probably a real galactagogue and might be useful in induction of lactation


Article
The Role of Parity on Some Macroscopical and Microscopical Variables in Placentas of Normal and Preeclamptic Women

Authors: Lina A. Hussain --- Malak A. Al-Yawer --- Huda M. Al-Khateeb
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 4 Pages: 578-583
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: The placenta is a dynamic organ, throughout gestation, it continuously undergoes different changes in structure and function to support the prenatal life. The anomalies of the placenta are usually associated with pregnancy complicated diseases which could lead to fetal complications. Hence, a careful examination of placenta in-utero as well as post-partum provides much insight into the prenatal health of the baby and the mother and can give information which could be useful in the management of complications in mother and the newborn, especially in a community like ours, where antenatal mothers still come unbooked to the labour room.OBJECTIVE: The aim of the present study was designed to elicit some morphometrical variables of delivered placentas ( both gross and microscopic) in normal and preeclamptic pregnancies with regard to the role of parity (birth order) as a physiological change in women's life that may affect placental morphometrical variables.PATIENTS AND METHODS: A total of twenty- four placentas were freshly collected. They were grouped into two major groups (normal and preeclamptic) and each group was further subdivided according to parity into primi and multi subgroups (6 placentas for each subgroup). The placentas were grossly examined for (shape, insertion of umbilical cord,diameter and central thickness). Then tissue samples were fixed, processed , sectioned and stained by heamatoxylin and eosin stain to study the following microscopical variables as number of (villi, syncytial knots and fetal capillaries) .RESULT:Studying placentas had circular to oval shape. The percentage of central insertion of umbilical cord was increased in control group, mainly in multi one. While marginal insertion was increased mainly in primi preeclamptic. There was a significant reduction in diameter of placentas of preeclamptic group, mainly at primi. The placental thickness was significantly increased in preeclampsia. Statistical analysis for histomorphometrical variables had got an increased in number of villi, syncytial knots and fetal capillaries with preeclampsia and parity. The number of fetal capillaries was significantly increased with preeclampsia (mainly in primi subgroup).CONCLUSION: In our study, the definite changes in macroscopical and microscopical variables in placentas of normal and preeclamptic women could be attributed to placental insufficiency especially in preeclamptic group and this may be a compensatory repair mechanisms to factors like hypoxia in order to provide better fetal growth.


Article
Subcutaneous Pancreatic Transplantation Shows Success In The Treatment Of Experimentally Induced Diabetes Mellitus In Golden Hamsters

Authors: Salim R Al-Ubaidy** ماهرة نوري، هدى الخطيب ، سالم رشيد العبيدي --- Huda M Al-Khateeb --- Mahera N Al-Shaik*
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2006 Volume: 48 Issue: 3 Pages: 287-292
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: to evaluate subcutaneous pancreatic transplantation in an experimentally induced diabetes mellitus in golden hamsters. Methods - many indexes (histological /using two stains, histochemical /using three enzymes, morphometrical and biochemical /blood glucose level) were employed in this assessment. Alloxan was used as ك-cytotoxic therapy (to induce diabetes mellitus), and cyclophosphamide was employed as immunosuppressive agent.
Results - high rate of success of transplantation (clearly evident by microscopical features of viable endocrine and exocrine tissue of transplanted pancreas and great tissue vascularization, normal enzymatic activities and significant lowering of blood glucose level) were elicited.
Conclusion – subcutaneous pancreatic transplantation should be thank about in treatment of diabetes mellitus.


Article
Study of H.Pylori in a Group of Iraqi Patients with Inflammatory Bowel Disease (Histological and Molecular Study)

Authors: Sinan S. Farhan --- Khalid A. Al-Khazraji --- ,Fadhil A. Al-Khafaji --- Huda M. Al-Khateeb --- et al.
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: supplement Pages: 734-741
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori is one of the commonest bacterial pathogens in human. The organism is associated with development of peptic ulcer diseases, lymphoproliferative disorders and gastric cancer.Helicobacter pylori could be isolated from patients with inflammatory bowel disease (IBD) but still the possibility of etiological link need further study thereforeOBJECTIVE:Is to assess the possible causal factor of H.pylori in development of inflammatory bowel disease, in addition to assess Cytotoxic associated gene A(CagA) gene status in H.pylori positive samples.PATIENTS AND METHODS:Study involved 120 patients of colonoscopically determined normal colon (n=90) & patients with IBD (n=30) including both Ulcerative colitis(n=16) and Crohn’s disease(n=14). Those patients is further divided into three age groups including <20 years group, 20-40 years group and >40 years group of Iraqi patients. Endoscopic specimens after histopathological confirmation of diagnosis will be tested for Biopsy Urease Test (BUT) and Hematoxylin & Eosin (H&E) methods (for detection of H. pylori) also detection of CagA mRNA using In Situ Hybridization technique with a biotin labeled probe (to specify pathogenic H. pylori)RESULTS:H.pylori detected in 36.7% (using BUT) and 30% (using H&E) in the colon of IBD patients and 33.3% (using BUT) and 26.7% (using H&E) in patients with normal colon (NC patients) as a control. Among our H.pylori positive patients, there was significant difference (P=0.036) regarding CagA status in which 25% were CagA positive of NC patients and 66.7% CagA positive H.pylori in IBD patients using In Situ Hybridization technique.CONCLUSION:H.pylori was isolated from nearby or the site of lesion of patients with inflammatory bowel disease although there was no statistical relationship between H.pylori and IBD. In addition CagA genes were more prominent in H.pylori that isolated from IBD patients than H.pylori of normal colon. Also there was no possible relationship between age and infection rate of H.pylori in both IBD & NC patient


Article
P16INK4a Overexpression in Cervical Biopsies Collected from Women with Normal and Equivocal Pap Smears
التعبير المبالغ فيه لمادة P16INK4a في خزعات عنق الرحم من نساء لديهم مسحات سليمة وملتبسة

Authors: Thanaa JM Al-Khishali ثناء جميل الخشالي --- Nada Salih Ameen ندى صالح أمين --- Huda M Al-Khateeb هدى مهدي الخطيب
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2016 Volume: 29 Issue: 1 Pages: 16-25
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Cervical cancer is the third most common cancer among women worldwide Immunohistochemical expression of p16INK4a has been relatively well established in cervical biopsy specimens.Objectives: Our hypothesis is to evaluate the usefulness of p16 immunostaining on tissue sections in borderline cases. Methods: This is a case-control study with a total of cervical samples from 60 women (25-66 years age) divided into three groups (I, II and III). The study was conducted at the Colposcopy Clinic in Baghdad Teaching Hospital – Medical City Complex, during the period from June 2013 through July 2014. The samples were analyzed by cytopathology, histopathology and p16INK4a immunostaining.Results: Group I showed negative p16 reaction. Among group II; one (5%) showed mild positive, 9 (45%) moderate positive and 10 (50%) high positive reactions. Group III specimens showed p16 positivity. Conclusion: p16INK4a immunohistochemistry is one of the best candidates for histologically indeterminate lesions.

الخلفية: سرطان عنق الرحم هو ثالث أكثر أنواع السرطان شيوعا بين النساء في جميع أنحاء العالم. لقد تم نسبيا اثبات التعبير المناعى المبالغ فيه لمادة p16INK4a في عينات من خزعات عنق الرحم. فرضيتنا هي تقييم فائدة الصبغة المناعية P16 على شرائح الأنسجة في الحالات الملتبسة أو غير المتأكد من تشخيصها.الطريقة: هذه دراسة الحالات الافرادية المقترنة بحالات ضابطة (case-control study) لمجموعه من عينات عنق الرحم من 60 امرأة (25-66 سنة العمر) مقسمة إلى ثلاث مجموعات (الأولى والثانية والثالثة). أجريت هذه الدراسة في عيادة التنظير المهبلي في مستشفى بغداد التعليمي - مجمع مدينة الطب، خلال الفترة من حزيران 2013 حتى تموز 2014. وقد تمت دراسة العينات عن طريق الفحص الخلوي، مقاطع لشرائح عن طريق علم أمراض الأنسجة، ومقارنتها بالصبغة المناعية لمادة p16INK4a. النتائج: أظهرت نتائج المجموعة الأولى رد فعل سلبي لصبغة P16. أما نتائج المجموعة الثانية فكانت كالاتي: واحد (5٪) أظهر رد فعل إيجابي ضعيف، وتسعة 9 (45٪) متوسط الإيجابية و10 (50٪) أظهر رد فعل إيجابي عالي. أما المجموعة الثالثة فقد أظهرت العينات إيجابية عالية لمادة p16.


Article
MORPHOLOGICAL STUDIES ON THE EFFECT OFNIGELLA SATIVA SEEDS ON THE MAMMARY GLAND OF MATURE MALE NORWAY RAT
دراسات شكليائية لتأثير بذور الحبة السوداء على الغدد اللبنية لذكر الجرذ النرويجي الناضج

Authors: Frial A. Al-Mahdawi فريال عبد المناف المهداوي --- Huda M. Al-Khateeb هدى مهدي الخطيب --- Abed H. Baraaj عبد حسن براج
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2009 Volume: 6 Issue: 1 Pages: 123-128
Publisher: Baghdad University جامعة بغداد

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Abstract

Nigella sativa seeds (NSS) are reputed traditionally and scientifically as a potent agent that promote milk secretion (galactagogue), but their mechanism of action is studied trivially. One suggestion was that these seeds are bio-transformed to sex hormones within the ovaries. Therefore, this investigation was designated to throw light on the action of these seeds in the absence of ovaries i.e. in male rats. Thirty Norway male albino rats were used in this investigation. They were divided into experimental (n=20,fed NSS 2g/ Kg body weight /day for 14 days) and control (n=10, fed placebo for 14 days). After sacrifice mammary gland and blood samples were obtained. Experimental rats revealed a significant increase (p (0.01>in weight, hypertrophied mammary gland lobules with dilated ducts and rise in serum prolactin level, as compared to the controls. The Mammogenic action of NSS is most probably conveyed via extra-ovarian pathway. Nigella sativa seeds have an estrogen and progesterone like action or their steroidal saponins may be biotransformed to these hormones and cause mammogenesis .

لبذور الحبة السوداء سمعة تقليدية و علمية كمدرة للحليب, لكن آلية عملها لم تدرس إلا نادرا. أشارت بعض الدراسات في هذا المجال إلى إن هذه البذور تتحول في المبايض إلى هرمونات جنسية. و لبيان صحة هذه الادعاءات صمم هذا البحث لدراسة مدى فعالية هذه البذور بغياب المبايض(اى في الذكور).تضمن البحث دراسة ثلاثون جرذ نرويجي ابيض ذكر. قسمت هذه الحيوانات إلى قسمين: حيوانات تجريبية (عددها = 20 جرذ, أعطيت مسحوق بذور الحبة السوداء لمدة 14 يوما) و حيوانات السيطرة (عددها = 10 جرذ, أعطيت الماء المقطر لمدة 14 يوما). بعد التضحية بالحيوانات, تم دراسة الغدد اللبنية نسجيا و دراسة الدم كيميائيا.أظهرت الحيوانات التجريبية زيادة معنوية إحصائيا في أوزانها, كما بينت زيادة في حجم فصيصات الغدد اللبنية و توسع في قنواتها و كذلك أشارت إلى زيادة في مستوى هرمون البرولاكتين في الدم مقارنة مع مجموعة السيطرة.نستنتج من هذه الدراسة بان النمو الحاصل في الغدد اللبنية عند اخذ بذور الحبة السوداء لا يمر من خلال تحولها في المبايض و إنما بطريقة أخرى ربما تكون من خلال المراكز العليا و هذا ما يحتاج إلى دراسة أخرى في المستقبل.


Article
Detection of H.pylori in a Group of Iraqi Patients with Colonic Cancer (Histological and Molecular Study)

Authors: Sinan S. Farhan ,Khalid A. Al-Khazraji --- Fadhil A. Al-Khafaji --- Huda M. Al-Khateeb ,Zahraa Q. Al-Kassam, Haider A. Al-Zubaidi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 1 Pages: 153-159
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: H. pylori is a one of the gastrointestinal organism which infects more than half the population of the world. H.pylori had been recognized as a class I human carcinogen by the International Agency for Research on Cancer. Recently H. pylori were detected in the mucosa of normal colon. Certain studies suggested a possible relationship between CagA (Cytotoxin Associated Gene A) positive H.pylori and Colonic Cancer while other studies did not find causal relationship between CagA positive H.pylori and Colonic Cancer. OBJECTIVE: We studied and isolated H.pylori from at or near by the site of lesion of Colonic Cancer and it is found to assess a possible relationship between CagA positive H.pylori and Colonic Cancer.PATIENTS AND METHODS: Study involved 30 patients of colonoscopically and Histopathologicaly determined Colonic Cancer (CC group) compared with patients with normal colon (n=90) as a control group (NC group). Patients divided into <20 years age group, 20-40 years age group and >40 years age group. Endoscopic specimens were tested for Biopsy Urease Test (BUT) and Hematoxylin & Eosin (H&E) method for detection of H. pylori also the detection of CagA mRNA using In Situ Hybridization technique with a biotin labeled probe.RESULTS: There was 66.7% H.pylori positive in patients with Colonic Cancer (by BUT) and 60% by (H&E) compared to 33.3% (by BUT) and 26.7% (H&E) in patients with normal colon (Control). A comparison between NC&CC groups regarding H.pylori infection using H&E method revealed significant difference (P=0.001).Using In Situ Hybridization technique, there was 66.7% CagA positive H.pylori in CC group in correspondence to 25% in NC group. CagA positive H.pylori infection between CC and NC groups revealed significant difference (P=0.008)CONCLUSION: H.pylori detected at or near by the site of lesion of Colonic Cancer. In addition it was found that there is a statistical relationship between CagA positive H.pylori infection and Colonic Cancer which suggested a possible role of CagA positive H.pylori in Colonic Cancer.


Article
Virulence estimation by calculation of relative expression of NESTIN in different grades of astrocytoma among Iraqi patients
تقدير العدوانية بواسطة حساب التعبير النسبي للموروثة NESTIN في الدرجات المختلفة للورم النجمي للمرضى

Authors: Khitam R.Alkhafaji د. ختام رزاق كاظم --- Huda M.Al-Khateeb د. هدى مهدي الخطيب --- Zainab Z.Saadoon د. زينب زاهد سعدون
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2019 Volume: 61 Issue: 3-4 Pages: 105-110
Publisher: Baghdad University جامعة بغداد

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Abstract

Background:Astrocytoma, is heterogeneous tumorof the nervous systemand studies on the virulence of thesetumors reveal that theirbehavior isled by small population of cells which arethe brain tumor stem cells (BTSCs) that drive the continuousproliferation and self-renewal. From the many markers that annotate BTSCs, arethe CD133, and NESTIN.Objectives:Using CD133, to immunolabelBTSCs niches in paraffin sections of astrocytoma then, extraction of thesecells, to calculate fold expression of NESTIN gene across the grades by real time PCR. Materials and methods: Paraffin blocks of four grades of primary astrocytoma have been selected from three age groups from Iraqi patients. The age groups were stratified into: children (0-17years) adults (18-49years) and older (50-80years). The samples were stainedwith CD133, thenpositive areaswereextracted to perform relative expressionof NESTIN geneby real time PCR Results:The expressionof NESTINfor all grades,increasedby tens of folds in relation to calibrator then this fold expression increaseddramatically in an ascendingmanner withincreasing of the grade in the same group. The fold expression of NESTIN gene in all grades was the highest in the adulthood (18-49) yearsgroup. Conclusion: Relative expression of NESTIN gene of BTSCs in different grades of astrocytoma can be a useful tool for the assessment of the virulence with ascending the grades. This expressionwas the highest at age (18-49) years

:الورم النجميورم متباين التكوين والراسات حول عدائية الأورام تكشف ان هذا التصرف وراءه مجموعة خلايا جذعية خاصة بالورم والتي تسبب التضاعف المستمر والتجدد الذاتي. من المعلمات التي تحدد الخلايا الجذعية الورمية هو الCD133 وكذلك ال NESTINالأهداف: باستخدام CD133لتعليم الخلايا الجذعية الورمية في النماذج الشمعية للاورام النجمية وبعدها استخلاص هذه الخلايا لحساب التعبير الجيني الضعفي لل NESTIN عبر الدرجات المختلفة بواسطة REAL TIME PCR المواد والطرائق: نماذج شمعية من اربع درجات من الورم النجمي الابتدائي اختيرت من ثلاث مجاميع عمرية من المرضى العراقيين. المجاميع العمرية صنفت الى اطفتا (0-17) بالغين (18-49) وكبار (50 -80). النماذج صبغت بCD133بعدها تم استخلاص المناطق الإيجابية لانجاز التعبير الجيني النسبي لNESTINالنتائج: التعبير الجيني للnestinلكل الدرجات ازداد بعشرات الاضعاف بالنسبة للقياس البدائي لها وكذلك هذ التعبير الضعفي قد تزايد بشكل ملحوظ وتصاعدي مع زيادة الدرجة. التعبير الضعفي للnetin كان الأعلى في مجموعة البالغينالاستنتاج:التعبير النسبي ل nestin للخلايا الجذعية الورمية في مختلف الدرجات للورم النجمي هو وسيلة مفيدة لتقييم العدوانية للورم مع تزايد الدرجة الورمية وهذا التعبير كان الأعلى في مجموعة البالغين (18-49 )مفتاح الكلمات : العدوانية، الورم النجمي ، NESTIN، درجات، اعم

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