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Study The Interaction between LH, FSH, and TSH with New Synthesized Magnetic Nanoparticles Coated with Dextran

Authors: Fatin Fadhel Al-Kazazz --- Hussain Kadhem Al-Hakeim --- Habiba Khdair Al-Aobaid
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 2 Pages: 421 -434
Publisher: Babylon University جامعة بابل

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Abstract

Nanoparticle (NP) was used in vivo and in vitro in contact with the body fluids that contain different types of proteins. The proteins have the ability to adsorb on NPs surface, and alter its activity due to the modification of the secondary and tertiary structures. In this study, the interaction between coated magnetic nanoparticles (MNP@Dextran) and the hormones (LH, FSH, and TSH) were studied in details. The possible change in the secondary and tertiary structure of these parameters is discussed. The (MNPs) were prepared using co-precipitation method of Fe3O4 followed by reacted these MNPs with Dextran under an optimum conditions to produce coated MNP@Dextran. Different concentrations of each hormone were incubated with a known amount of MNP@Dextran and the quantity of the hormones adsorbed was calculated. The experiments were repeated at different temperatures to calculate the thermodynamic parameters. Secondary structures of hormones were examined using circular dichroism (CD).Tertiary structures of protein were measured using fluoroscopy technique.The adsorption process obeyed Freundlich adsorption isotherm indicating heterogeneity of the MNP@Dextran surface,and it associated with the changing in the secondary and tertiary structure of hormone after adsorption on MNP@Dextran.

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