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Article
Relationship between Thyroid Function, Cystatin C and Different Oxidative Stress in Iraqi Patients with Chronic Kidney Disease

Author: Israa Ghassan Zainal
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 2 Pages: 337 -346
Publisher: Babylon University جامعة بابل

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Abstract

Chronic Kidney Disease (CKD) is associated with slightly higher frequency prevalence of primary hypothyroidism , but at the same studies on thyroid hormone status in uremic patients has reported conflicting results. This study was undertaken during the period from February 2014 to January 2015to quantify thyroid hormones (T3,T4 and TSH) ,cystatin C, different oxidative stress parameters like serum Ceruloplasmin (CP), Carbonyl, Thiol and total protein (TP) and four trace elements (Molybdenum (Mo), Cadmium (Cd), Manganese (Mn) and Magnesium (Mg) and explore correlation between these parameters in (75) non- dialyzed CKD patients’ verses (52) healthy controls. Results indicated that the levels of (T3, T4,CP, TP,thiol,Mo ,Cd and Mg were significantly reduced (p≤ 0.05)while the levels of TSH ,cystatin C ,carbonyl and Mnweremildly significantly increase(p≤ 0.05) in patients group compared to healthy controls.The correlation coefficient ( r ) test is used to describe the association between these parameters,T3 and T4 were negatively correlated with cystatin C ,carbonyl, Mn and Mg, positively correlated with(thiol and protein) .T4 positively correlated with CP while T3 not correlated with CP.TSH positively correlated with (CP, carbonyl,thiol,protein), finally, CP negatively correlated with cystatin C and positively correlated with Mn and Mg.

Keywords

CKD --- antioxidants --- cystatin C --- ceruloplasmin --- Mo --- Cd --- Mg --- thyroid hormones


Article
Human Carbonic Anhydrase: Purification and Characterization Study in Thalassemia Major Patients Compared to Healthy Subjects

Author: Salwa Saleh Hussein, Israa Ghassan Zainal
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 4 Pages: 349-356
Publisher: Babylon University جامعة بابل

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Abstract

Background: Carbonic anhydrase (CA) catalyzes the reversible reaction of converting carbon dioxide to bicarbonate. Objective: This studywas aimed to isolate and purify human erythrocytes CA and study its physicochemical properties of the enzyme reaction for ß‑thalassemia majorpatients. Materials and Methods: The blood samples included 61 samples of blood (31 males and 30 females) from ß‑thalassemia patientsvisited Azadi Hospital/Kirkuk city. Healthy individuals as control group included 40 participants. The separated fractions were obtained usingfour steps: extraction by ethanol and chloroform, ammonium sulfate precipitation, dialysis, and gel filtration chromatography; finally, the CAwas analyzed by polyacrylamide gel electrophoresis. Results: The CA activity showed significant (P ≤ 0.05) decrease, total protein showednonsignificant (P ≥ 0.05) increase, and specific activity significantly (P ≤ 0.05) increased in patients group compared to healthy individuals.CA was partially purified with a factor of 22.5 and 18 by extraction with ethanol and chloroform and 1.5,1.4 for Fraction I and 1,2 for FractionII using gel filtration chromatography. The optimum conditions for the CA reaction in patients group were enzyme concentration (6 μl),substrate concentration (6 Mm), pH = 7.4, and temperature 37°C. The electrophoresis study indicated that the bands of CA in patients groupshowed bands with less intensity than the bands in healthy individuals. Conclusion: The best method to purify CA from human erythrocyteswith high recovery and fold of purification was ethanol–chloroform extraction.


Article
Serum Protein Levels and Albumin/Globulin Ratio in Patients with Uterus, Ovary, and Breast Tumors Compared to Healthy Women in Kirkuk City

Author: Israa Ghassan Zainal, Ebaa Ghassan Zainal
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2019 Volume: 16 Issue: 2 Pages: 136-140
Publisher: Babylon University جامعة بابل

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Abstract

Background: Gynecological cancer is one of the most serious public health problems in the world with main gynecological cancers, uterus,breast, and ovarian, causing morbidity with severe suffering and a high mortality. Objective: This study aimed to investigate the levels of thetotal protein (TP), albumin (Alb), globulin (Glo), and Alb/Glo ratio in the female patients with (uterus, ovary, and breast) cancer comparedto healthy females. Materials and Methods: This study included 120 females with (28 uteri, 31 ovaries, and 25 breasts) cancer patientsand 36 healthy control participants, with age ranges between (20 and 42) years. Results: Alb levels significantly (P ≤ 0.05) decreased in allpatient samples except those with uterus cancer who showed significant increase (P ≤ 0.05) compared to healthy participants, and Glo levelssignificantly (P ≤ 0.05) decreased for uterus and breast cancer samples and nonsignificant (P ≤ 0.05) decrease in patients with ovary cancercompared to healthy participants; finally, the ratio of Alb./Glo. showed significant (P ≤ 0.05) decrease in all patients group except patientswith breast cancer showed significant increase (P ≤ 0.05) compared to healthy participants. The results of correlation coefficient indicated thatthere were significant (P ≤ 0.05) positive correlations between TP and Glo for uterus and breast cancer patients and between TP and Alb forovary cancer patients. Conclusion: The ratio of Alb/Glo was significantly (P ≤ 0.05) decreased in all patients group except patients with breastcancer who showed significant increase (P ≤ 0.05) compared to healthy participants which could be used in the diagnosis of these patients.


Article
and Medical In vitro anti-cancer activity of Doxycycline on some human cancer cell lines
فعالية عقار الدوكسيسيكلين على بعض الخطوط السرطانية البشرية (في الزجاج)

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Abstract

Doxycycline (DC) is an antibiotic that is used in the treatment of number types of infections diseases caused by bacteria and protozoa. Doxycycline is a kind of second-generation tetracyclines which is commonly used to treat a variety of in- fections. Three cancer cell lines include: human cerebral glioblastoma-multiforme (AMGM) at passages 75-84, human cervi- cal cancer (HeLa) at 70 passages, and human pelvic rhabdomyosarcoma (RD) at 75 passages cell lines were used in this study. The results showed that DC exerted signi cant cytotoxic effects with all concentrations used (50,100,150,200,250,300,350 and 400) μg/ml on all types of cell lines. Because of cytotoxic activity, good pharmacokinetic characteristics and the safety of drug which used for many years in the treatment of infectious disease, we can conclude that these characteristics make DC a valuable treatment for many types of cancer.

إلدوكسيسيكلين مضاد حيوي من الجيل الثاني للتتراسيكلينات يستخدم في ع ج العديد من ا مراض التي تسببها البكتيريا والبروتوزوا. استخدمت في هذه الدراسة ث ثة خطوط لخ يا سرطانية هي: glioblastoma الدماغ البشري- متعدد ا شكال ) AMGM( عند تمريرة ال84-75, سرطان عنق الرحم البشري )HeLa( عند مقطع ال70 و سرطان الخ يا العضلية المخططة البشرية )RD( عند تمريرة ال75. أشارت النتائج الى ان الدوكسيسيكلين يظهر تأثير معنوي للسمية عند التراكيز المستخدمة وهي )50, 100, 150, 200, 250, 300, 350 و 400( μg/ml على جميع انواع الخ يا قيد الدراسة. نظرا” للفعالية السمية والخواص الدوائية الجيدة وس مة الدواء حيث استخدم لسنوات عديدة في ع ج العديد من ا مراض المعدية, يمكننا ا ستنتاج بان إلدوكسيسيكلين ع ج جيد نواععديدة من السرطان.

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