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Article
Direct detection of Shiga toxin producing by Escherichia coli by real-time PCR
الكشف المباشر عن سموم الشيكا المنتجة بواسطة الاشريشيا كولاي بتقنية الوقت الحقيقي لتفاعل سلسلة البلمرة

Author: Jameela Radi Esmaeel جميلة راضي اسماعيل
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2015 Volume: 14 Issue: 2 Pages: 78-82
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Shiga toxin-producing Escherichia coli (STEC) are defined as strains of E. coli that produce Shiga toxins (stx), which known as important causes of diarrhea in sheep and cattle. This study was conducted to determine of Shiga toxin 1 producing E. coli isolates from diarrheal samples of sheep and cattle. Samples were collected from different fields in Diwanyia city. A total of 50 diarrhea samples (25 of sheep and 25 of cattle) were subjected to bacterial DNA extraction by using (AccuPrep® Stool DNA Extraction Kit). The extracted DNA subjected to Real-Time PCR technique for detection of Shiga toxin 1 (stx1) gene. Results display that sheep are more prevalence to shedding the Shiga toxin 1 producing E. coli in (5/25) (20%), while in cattle (2/25) (8%) positive samples. In conclusion the using of Real-Time technique was shown high specific and rapid method in direct detection of (stx1) gene and most the sheep and cattle which infected by diarrhea carried the Shiga toxin 1 producing E. coli.

تعرف الاشريشيا كولاي المنتجة لسموم الشيكا بانها العزلات التي تنتج سموم الشيكا والتي تعرف بانها من الاسباب الهامة للإسهال في الاغنام والابقار. اجريت هذه الدراسة لتحديد سموم الشيكا 1 المنتجة من عزلات الايشريشيا كولاي في عينات الاسهال في الاغنام والابقار والتي تم جمعها من حقول مختلفة في مدينة الديوانية. تم اخذ 50 عينة اسهال (25 من الاغنام و25 من الابقار) وتم استخلاص الحامض النووي باستخدام AccuPrep® Stool DNA Extraction Kit. وتم اخضاع الحامض النووي المستخلص لتقنية الوقت الحقيقي لتفاعل سلسلة البلمرة للكشف عن جينات سموم الشيكا . اظهرت النتائج ان الاغنام هي الاكثر بطرح الجرثومة المنتجة لسموم الشيكا (525) بنسبة (20%)عينات ايجابية فيما اظهرت الابقار (225) وبنسبة (8%) عينات ايجابية. نستنتج من الدراسة ان استخدام تقنية الوقت الحقيقي لتفاعل سلسلة البلمرة ابدى انها تقنية عالية النوعية وسريعة في الكشف عن جين سموم الشيكا1. وان معظم الاغنام والابقار المصابة بالإسهال كانت حامله للعزلة .


Article
Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Authors: Jameela Radi Esmaeel --- Jenan Nadhim Sadeq
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2018 Volume: 17 Issue: 2 Pages: 49-52
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Hemolysin protein is exotoxin produced by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using a specific primer. This gene potent the pathogenesis of Klebsiella pneumonia. The primer was designed in this study by NCBI-GenBank and primer3 plus. (Bioneer Company provided the primers. Korea). Molecular detection of isolates, which give away specific PCR products of 505bp for hly gene, hemolysin gene, was detected in 70% (14/20).

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Article
Isolation Enterohemorrhagic Escherichia coli from gall bladder of buffaloes and determine its sensitivity for antibiotics

Authors: Ali Anok Najum --- Jameela Radi Esmaeel --- Balsam Miri Mizher
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 32-35
Publisher: University of Kufa جامعة الكوفة

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Abstract

Enterohemorrhagic Escherichia coli (EHEC) has been associated with hemorrhagic colitis, a severe form of diarrhea, and with hemolytic uremic syndrome, This study was preformed to find EHEC in gall bladder of buffaloes , (150 gall bladder) samples were collected from slaughter house and cultured on MacConky agar then identified by culture on Cefixim Tellurite Sorbitol-MacConkey, agar then tested with several types of antibiotics and serotyping of isolates to determine EHEC, in addition to conjucation methods were preformed .Results showed that 13 isolates of E.coli were obtained and 6 strainS were grown on Cefixim Tellurite Sorbitol-MacConkey agar and positive for specific antisera of (EHEC O157:H7), and all strains were only sensitive for nitrofurane, When conjucation done all recipent cell were resistant for antibiotics: (ampicillin,streptomycin,sulfonamide,trimthoprine,tetracycline,chloramphenicol,gentamycin and amikacin).


Article
Molecular detection of invA, ssaP in Salmonella typhimurium isolated from chicken in Al-Qadisiyah Province

Authors: Azhar Abdulsada Neama --- Jameela Radi Esmaeel --- Jinan Nadhim Sadeq
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 8-13
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Salmonella is considered the most important cause of foodborne diseases. Identification of Salmonella typhimurium evaluated using bacteriological assay followed by PCR technique. In this study, 40 intestinal content specimens of poultry were collected randomly from different farms of Al-Qadisiyah province. Outs of 40 samples obtained, 14 isolates (35 %) were detected as Salmonella typhimurium according to conventional bacteriological characteristics, the Vitek 2 system for identification and molecular assays. Two sets of primers were designed for detecting invA and ssaP genes. These genes potent the virulence of Salmonella typhimurium. The primers were made in this study by using NCBIGenBank and design online. The primers were made by (Bioneer) company, Korea. Molecular assay of the isolates gives away specific PCR products of 677bp for the invA gene and 314bp for ssaP gene. The invA genes were amplified in 11 (78.5%) out of 14 isolates of Salmonella typhimurium, while ssaP genes were amplified in 10 (71.4%) out of 14 isolates of Salmonella typhimurium. The result of the study confirms the ability of these specific primers for detection of salmonella typhimurium in samples of chicken as well as the rapidly and sensitivity of the PCR method as a good tool for bacteriological identification.

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Article
Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.
الكشف عن عوامل ضراوة الكلبسيلا الرئوية المعزولة من الابقار باستخدام تقنية سلسلة تفاعل البلمرة

Authors: Jameela Radi Esmaeel جميلة راضي اسماعيل --- Jinan Nadhim Sadeq جنان ناظم صادق
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2017 Volume: 8 Issue: 1 Pages: 231-239
Publisher: University of Kufa جامعة الكوفة

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Abstract

Identification of K. pneumoniae was avaluated using conventional microbiological characteristics and molecular assays .Milk specimens were collected from cattle suffered from clinical symptoms of mastitis from different farms of AL-Qadisyiah province.Out of 45 samples obtained, 20 isolates ( 44.4 %) were detected as K. pneumonia according to morphology of colonies and biochemical features.Molecular detection of Klebsiella pneumoniae based 16S rRNA gene for determination two virulence factor genes(rmpA,magA) by using specific primer.These genes potent the pathogenesis of Klebisella pneumonia. The primers were made in this study by using NCBI-GenBank and primer3 plus design online. The primer is made by company in Korea (Bioneer).Moleculer detection of isolates which give away specific PCR products of of 312bp for magA gene and 835bp for rmpA gene .The magA and rmpA genes were amplified in six (30%)and five(25%) isolates out of 20 isolates of Klebsiella pneumonia.

تم تشخيص الكلبسيلا الرئوية الخصائص الميكروبيولوجية التقليدية وطرق القياس الجزيئي .تم جمع عينات الحليب من ابقار تعاني من علامات سريرية لالتهاب الضرع من حقول مختلفة في محافظة القادسية .من بين 54 عينة تم الحصول عليها .تم الكشف عن 02 عزلة بنسبة ) 5545 %(.وتم تحديد الكلبسيلا الرئوية علىاساس شكل المستعمرات والاختبارات الكيموحيوية .تم التشخيص الجزيئي للكلبسيلا الرئوية 16srRNA بالاستناد على جين باستخدام بادئاتمتخصصة (MagA,rmpA ) لتحديد عاملين من عوامل الضراوة وهما صممت هذه البادئات في الدراسة باستخدامبنك الجينات وصممت ثلاث بادئات جهزت بواسطة كوريا .تم الكشف الجزيئي للعزلات وحددت نواتج(Bioneer company) شركة210 و 524 (للجينين على التوالي . )bp PCR ظهر تضخيم الجينات في ستة عزلات بنسبة ) 22 %( وخمسة عزلات بنسبة ) 04 %( من اصل 02 عزلة منالكلبسيلا الرئوية .

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