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Article
Serum level evaluation of interleukin-18 in obese women with polycystic ovary syndrome

Authors: Mohamed Ismail Ibrahim --- Jinan M. Al-saffar
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 4B Pages: 1989-1994
Publisher: Baghdad University جامعة بغداد

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Abstract

This study is designed to measure the level of interleukin (IL) 18 in polycystic ovary women and its association with obesity. In this study, blood samples from 50 women with PCOS and 30 healthy control women were collected from AL-Yarmouk Teaching, Baghdad Teaching hospitals During January 2018 - March 2018 for estimation of their serum level of IL18 by using enzyme-linked immunosorbent assay (ELISA) technique and evaluation serum levels of luteinizing hormone (LH), Follicle-stimulating hormone (FSH), Testosterone, prolactin (PRL) and Estradiol (E2) by using Electrochemiluminescence immunoassay (ECLIA). The results showed that there is a highly significant increase (P < 0.001) in serum level of IL18 in PCOS women than in healthy control group. As well as, the results of IL-18 value according to Body mass index (BMI) showed significant difference (P<0.05) between BMI and IL-18 level in all the PCOS patient subgroups (normal weight, overweight and obesity). Also, there was a significant increase in LH, FSH, PRL and T, and significant decrease in E2 was detected in PCOS patients.


Article
Molecular Characteristics of Multidrug Resistant Acinetobacter baumannii Isolated from Baghdad Hospitals
الخصائص الجزيئية لبكتريا Acinetobacter baumannii متعدده المقاومة للمضادات الحيوية والمعزولة من مستشفيات بغداد

Authors: Khitam K. AL-Masoudi ختام خالص المسعودي --- Jinan M. AL-Saffar جنان محمد الصفار --- Nuha J. Kendla نهى جوزيف قندلا
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 2B Pages: 1394-1399
Publisher: Baghdad University جامعة بغداد

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Abstract

A total of 100 clinical sample from (urine, sputum and swabs of wound , burn and ear) were collected from patients in different hospitals of Baghdad during the period from December 2013 to May 2014. 15 isolates (15%) identified belong to Acinetobacter baumannii, swabs of wounds were represented in high percentage of A.baumannii isolates (40%) while percentage of other samples were variable. Susceptibility of 15 A.baumannii isolates were tested toward 16 different Antimicrobial agents, the results showed all isolates were multi drug resistant. In addition, Polymerase Chain Reaction Technique (PCR) was performed to detection the resistance genes encoding the Oxacillinases enzymes. The PCR analysis showed that the presence of insertion sequence (ISAba1) in 13 isolates whereas blaoxa51like gene was represented in 12 isolates. Furthermore, the results of detection other genes were not appear any amplification for all A.baumannii isolates with genes encoded for bla oxa 58 and bla oxa 143 enzyme.

شملت الدراسة 100 عينه سريريه ومن مصادر مختلفة (الإدرار،القشع،مسحات الجروح،مسحات الحروق ومسحات الإذن) من مرضى مستشفيات مختلفة في بغداد، خلال الفترة الممتدة من كانون الأول 2013 حتى مايس 2014.،شخصت 15 عزله(15%) تعود إلى Acinetobacter baumannii وكانت أعلى نسبه تشخيص تعود إلى مسحات الجروح(40%) وتغايرت نسبه العزل في مصادر العينات الأخرى.اختبرت حساسية عزلات بكتريا Acinetobacter baumannii باتجاه 16 مضاد حيوي، أظهرت نتائج الاختبار أن جميع العزلات كانت متعدده المقاومةللمضادات الحيويه. استخدم تقنيه Polymerase Chain Reaction Technique (PCR) للتحري عن امتلاك العزلات قيد الدراسة على الجينات التي تشفر إلى g(Oxacillinase) فوجد 13 عزله تشفر للتسلسل ISAba1، بينما 12 عزله كانت تحتوي على bla oxa 51 gene إما bla oxa58 gene and bla oxa 143 gene أظهرت نتيجة سالبه لكل العزلات.

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