research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
Molecular detection of invA, ssaP in Salmonella typhimurium isolated from chicken in Al-Qadisiyah Province

Authors: Azhar Abdulsada Neama --- Jameela Radi Esmaeel --- Jinan Nadhim Sadeq
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 8-13
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

Salmonella is considered the most important cause of foodborne diseases. Identification of Salmonella typhimurium evaluated using bacteriological assay followed by PCR technique. In this study, 40 intestinal content specimens of poultry were collected randomly from different farms of Al-Qadisiyah province. Outs of 40 samples obtained, 14 isolates (35 %) were detected as Salmonella typhimurium according to conventional bacteriological characteristics, the Vitek 2 system for identification and molecular assays. Two sets of primers were designed for detecting invA and ssaP genes. These genes potent the virulence of Salmonella typhimurium. The primers were made in this study by using NCBIGenBank and design online. The primers were made by (Bioneer) company, Korea. Molecular assay of the isolates gives away specific PCR products of 677bp for the invA gene and 314bp for ssaP gene. The invA genes were amplified in 11 (78.5%) out of 14 isolates of Salmonella typhimurium, while ssaP genes were amplified in 10 (71.4%) out of 14 isolates of Salmonella typhimurium. The result of the study confirms the ability of these specific primers for detection of salmonella typhimurium in samples of chicken as well as the rapidly and sensitivity of the PCR method as a good tool for bacteriological identification.

Keywords


Article
Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec.
الكشف عن عوامل ضراوة الكلبسيلا الرئوية المعزولة من الابقار باستخدام تقنية سلسلة تفاعل البلمرة

Authors: Jameela Radi Esmaeel جميلة راضي اسماعيل --- Jinan Nadhim Sadeq جنان ناظم صادق
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2017 Volume: 8 Issue: 1 Pages: 231-239
Publisher: University of Kufa جامعة الكوفة

Loading...
Loading...
Abstract

Identification of K. pneumoniae was avaluated using conventional microbiological characteristics and molecular assays .Milk specimens were collected from cattle suffered from clinical symptoms of mastitis from different farms of AL-Qadisyiah province.Out of 45 samples obtained, 20 isolates ( 44.4 %) were detected as K. pneumonia according to morphology of colonies and biochemical features.Molecular detection of Klebsiella pneumoniae based 16S rRNA gene for determination two virulence factor genes(rmpA,magA) by using specific primer.These genes potent the pathogenesis of Klebisella pneumonia. The primers were made in this study by using NCBI-GenBank and primer3 plus design online. The primer is made by company in Korea (Bioneer).Moleculer detection of isolates which give away specific PCR products of of 312bp for magA gene and 835bp for rmpA gene .The magA and rmpA genes were amplified in six (30%)and five(25%) isolates out of 20 isolates of Klebsiella pneumonia.

تم تشخيص الكلبسيلا الرئوية الخصائص الميكروبيولوجية التقليدية وطرق القياس الجزيئي .تم جمع عينات الحليب من ابقار تعاني من علامات سريرية لالتهاب الضرع من حقول مختلفة في محافظة القادسية .من بين 54 عينة تم الحصول عليها .تم الكشف عن 02 عزلة بنسبة ) 5545 %(.وتم تحديد الكلبسيلا الرئوية علىاساس شكل المستعمرات والاختبارات الكيموحيوية .تم التشخيص الجزيئي للكلبسيلا الرئوية 16srRNA بالاستناد على جين باستخدام بادئاتمتخصصة (MagA,rmpA ) لتحديد عاملين من عوامل الضراوة وهما صممت هذه البادئات في الدراسة باستخدامبنك الجينات وصممت ثلاث بادئات جهزت بواسطة كوريا .تم الكشف الجزيئي للعزلات وحددت نواتج(Bioneer company) شركة210 و 524 (للجينين على التوالي . )bp PCR ظهر تضخيم الجينات في ستة عزلات بنسبة ) 22 %( وخمسة عزلات بنسبة ) 04 %( من اصل 02 عزلة منالكلبسيلا الرئوية .

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2017 (2)