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INCIDENCE OF METHICILLIN RESISTANT STAPHYLOCOCCI IN BACTERIAL CONJUNCTIVITIS

Authors: Mokhtar A Al-Hammadi --- Khalid I Al-Mearaj --- Hadeel T Al-Hadithi
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2006 Volume: 12 Issue: 1 Pages: 98-99
Publisher: Basrah University جامعة البصرة

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Abstract

A total of 100 conjunctival samples were cultured to investigate the bacteriologic outcome, focusing on the incidence of methicillin resistant staphylococci. Bacteria was identified in 91 cases (91%). Cultural and clinical diagnosis were found 100% compatible in neonates, 96.6% in children and 86% in adult patients. Staphylococci were isolated from 65 cases (71.4%). Methicillin resistant staphylococci (MRS) were identified in 37 cases (40.6%) of bacterial conjunctivitis, 22 of which (59.5%) were methicillin resistant coagulase positive staphylococci (MRCOPS) and 15 (40.5%) were methicillin resistant coagulase negative staphylococci (MRCONS). This study highlights the need for more work to establish the role of MRS commensals and ocular infections.

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Article
THE PREVALENCE OF RETINOPATHY AMONG PATIENTS WITH SICKLE CELL ‎DISEASE

Authors: HAMDI S ABDUL-KHADER, --- KHALID I AL-MEARAJ, --- SARKIS K STRAK
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2005 Volume: 11 Issue: 2 Pages: 102-107
Publisher: Basrah University جامعة البصرة

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Abstract

Sickle cell anemia is an inherited disorder characterized primarily by chronic hemolytic anemia ‎and vaso-occlusive crises. It affects millions of people throughout the world. There is no tissue or ‎organ spared from injury by sickling disorder including the retina. ‎‎ This studt aimed to determine the prevalence of retinopathy among patients with sickle cell disease.‎‎ The study was done on 120 subjects, 60 patients and 60 healthy control. Beside electrophoretic testing, ‎all subjects underwent careful ophthalmoscopic examination (direct and indirect) by the same examiner.‎‎ The American academy of Ophthalmology criteria for diagnosing and staging of sickle retinopathy was ‎followed in this study. ‎‎ Retinopathy was more common patients with sickle cell disease (16%), than in control group (3%). ‎Those with SF hemoglobin were seem to be affected more than the other studied groups (AS, SS). Male ‎patients and those who were above 40 years showed more prevalence of retinopathy.‎

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Article
VANCOMYCIN RESISTANCE AND BIOFILM FORMATION AMONG METHICILLIN RESISTANT COAGULASE NEGATIVE STAPHYLOCOCCI ISOLATED FROM CONJUNCTIVITIS
المقاومة للفانكومايسين وإنتاج الغشاء الحيوي بين المكورات العنقودية السالبة للتجلط المقاومة للمثسلين المعزولة من المصابين بالتهاب المنظمة الجرثومي

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Abstract

ABSTRACTFifteen isolates of methicillin resistant coagulase negative staphylococci (MRCONS) recovered fromfifteen cases of bacterial conjunctivitis were tested for their susceptibilities to ten antibiotics, by discdiffusion method, the extent of their susceptibilities to vancomycin by agar dilution method and, theircapability for producing biofilm. Multiple antibiotic resistance was clearly recognized among allMRCONS and one strain was found resistant to all antibiotics. Six out of the 15 strains (40%) exhibitedintermediate resistance to vancomycin (8-16 μg/ml) and one isolate (6.7%) recovered from adult agegroup was fully resistant to vancomycin (32 μg/ml). Biofilm production was expressed by 11 out of the 15strains. These constituted seven isolates of VRCONS (100%) and four isolates out of MRCONS (50%).The association between biofilm formation and antibiotic resistance reflects clinical significance of theseisolates and the need for determination of antibiotic susceptibility directly against biofilm-associatedorganisms.

اختبرت 15 عزلة للمكورات العنقودية السالبة للتجلط والمقاومة للمثسلين من 15 حالة التهاب المنظمة الجرثومية لمعرفه حساسيتها تجاه عشرة مضادات حيوية بطريقة انتشار القرص ولتقدير مدى الحساسية للفانكومايسين بطريقة التخفيف بالاكار ومن ثم تحديد قدرتها على أنتاج الغشاء الحيوي كعوامل محددة لضراوة العزلات. بطريقة انتشار القرص أظهرت جميع العزلات مقاومة متعددة للمضادات الحيوية بينها عزلة واحدة مقاومة للفانكومايسين (30ug). اما بطريقة التخفيف بالاكار فقد أظهرت سبعة عزلات مقاومة للفانكومايسين بينها ست عزلات (40%)بالمقاومة المتوسطة (8-16 ug/ml) والعزلة السابعة (من مجموعة البالغين) كانت مقاومة تماماً (6.7%) للفانكومايسين (32ug/ml). تمكنت احد عشر عزلة من إنتاج الغشاء الحيوي و من بينها العزلات السبعة المقاومة للفانكومايسين (100%). يعكس الترابط, بين أنتاج الغشاء الحيوي والمقاومة المتعددة للمضادات الحيوية عامة والفانكومايسين خاصة, الأهمية السريرية لهذه العزلات والحاجة إلى تحديد الحساسية للمضادات الحيوية للعزلات المكونة للغشاء الحيوي.

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