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Inhibition of biogenic hydrogen sulfide produce by Sulfate Reducing Bacteria isolated from oil fields in Basra by nitrate based treatment

Authors: Wijdan H. Al-Tamimi --- Kawther H. Mehdi
Journal: Journal of Petroleum Research & Studies مجلة البحوث والدراسات النفطية ISSN: 22205381 Year: 2017 Issue: 15 Pages: 88-106
Publisher: Ministry of Oil وزارة النفط

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Abstract

The present study included using of Bio Competitive Exclusion BCX technology in treatment of biogenic production of hydrogen sulfide H2S by Sulfate Reducing Bacteria SRB in batch cultures, injection of nitrate and nitrite promotes the growth of Nitrate Reducing Bacteria NRB which outcompete the Sulfate Reducing Bacteria SRB on carbon sources. Mix cultures of SRB and NRB were isolated from produce water of oil production facilities in Iraq including Nahran Omer and Al lahis oil fields. The activity of NRB and SRB were determined by measured the concentration of nitrate, nitrite and sulfide by using spectrophotometer and bacterial counts in three tube MPN technique. The results showed that NRB had a certain inhibitory effect on the growth of SRB and sulfide production under adding NRB nutrient nitrate and nitrite at all concentrations 200, 400, 600, 800 and 1000 mg/l, activity of NRB increased after treatment, all nitrate and nitrite consumption during 2 - 3 days of incubation. The highest inhibition of H2S production was at concentration 1000 mg/l of nitrate where the results showed that there was significant decrease in sulfide level and number of bacteria 34 mg/l and 34.8 cell/ ml respectively after 10 days of treatment.

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Article
ISOLATION OF PLASMID DNA FROM STREPTOMYCES SP. BACTERIA AND ESCHERICHIA COLI PBR322 TRANSFORMATION
عزل الــ DNA البلازميدي من بكتريا Streptomyces sp. ونقله الى Escherichia coli pBR332

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Abstract

The local Streptomyces sp. strain showed an ability to produce antimicrobial metabolite active against standard strains, in primary and secondary screening. The produced antibiotic was extracted, purified and identified as a peptide antibiotic produced about 1.4g/L in 7 days incubation period, and its LD50 was 5500. There was an inverse effect for orange acridine dye on the grown colonies number of S. sp., the 28 g/ml dye concentration was chosen as the best concentration because it led to colonies killing by 95%. Plasmid DNA extracted from S. sp. and then transformed to E. coli pBR 322, the E. coli pBR 322 showed negative results against the standard strains in primary screening before plasmid DNA transformation, while transformed E. coli pBR322 showed positive results. The antibiotic produced by trans. E. coli pBR322 was extracted, purified and identified by the same ways, which gave the same antibiotic produced by S. sp. with an increase of 2.2 g/L in the quantity and shorter period of time (2 days).

اظهرت العزلة المحلي Streptomyces sp. القدرة على انتاج مادة ذات فعالية مضادة للجراثيم القياسية في الغربلة الاولية والثانوية, تم عزل وتنقية وتشخيص المضاد المنتج وكان ذوطبيعة ببتيدية وامتلك نصف جرعة قاتلة 5500 ملغم/كلغم, وانتج بكمية محدودة 1.4غم/لتر في سبعة أيام. اظهرت نتائج المعاملة بالاكردين ان عدد المستعمرات النامية يتناسب عكسيا مع كمية الاكردين المضافة للوسط ، حيث بلغت 95%نسبة القتل عند تركيز 28μg/ml حيث تم اختياره كتركيز افضل لترحيل البلازميد. استخلص الـDNA البلازميدي من S. sp.. ومن ثم نقل البلازميد الى بكتريا الـE. coli pBR322 حيث اظهرت هذه البكتريا صفة انتاج مضاد بكتيري في الغربلة الاولية لم تكن تمتلكها قبل عملية النقل. ومن ثم تم عزل, تنقية وتشخيص المضاد المنتج من الـE. coli pBR322 trans. بنفس الطريقة المستعملة لعزل, تنقية وتشخيص المضاد المنتج من S. sp. ، وكان هذا المضاد هو نفس المضاد المنتج من بكتريا S. sp. وبكمية اكبر2.2غم/لتر في فترة زمنية قصيرة (يومين).

Keywords

Peptide --- DNA --- Fungi

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