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Article
Expression of RANKL by dental cells during eruption of mice teeth

Authors: Lubna K. Jassim لبنى جاسم --- Athraa Y. Al- Hijazi عذراء يحيى الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 1 Pages: 76-81
Publisher: Baghdad University جامعة بغداد

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Abstract

Background : In order for a tooth to erupt, two obvious requirements are needed. First, there has to be alveolar boneresorption of the bone overlying the crown of the tooth such that an eruption pathway is formed. Second, resorptionof bony crypt and apposition of new one, third, there has to be a biological process that will result in the toothmoving through this eruption pathway.The amniotic sac contains a considerable quantity of stem cells. Theseamniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for humanapplication. Receptor activator of nuclear factor kappa B ligand (RANKL) is concentrated on bone biology, morespecifically bone metabolism. RANKL plays a vital role in osteoclastogenesis for bone resorption. This study aimed toevaluate the expression of RANKL marker by dental cells during eruption of the teeth.Materials and Methods: : forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cellsin the anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 micefor each period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: The present results localized and identified RANKL marker in 3 areas of developing tooth of the studied groupsincludes overlying, surrounding and apical bone. Positive RANKL with high significant value expressed by osteoclastof overlying bone in Amnion group followed by Control at day 4. In surrounding bone positive expression of RANKLillustrated to be highest in Control followed by Amniotic fluid at day 10.Apical bone shows positive expression ofRANKL in amniotic fluid group and it records to be the highest value in comparison to studied groups at day 10.Conclusion Expression marker RANKL illustrates that amniotic fluid group has a high expression of RANKL in osteoclastsurrounding and apical bone areas while control expressed RANKL in osteoclast of overlying bone. The present resultsopened clinical hopes in dental tissue engineering by application of autologous amniotic fluid and chorion cells

Keywords

RANKL --- tooth eruption


Article
Immunohistochemical study of CD34 in tooth eruption by using amniotic stem cells

Authors: Lubna K. Jassim لبنى جاسم --- Athraa Y. Al- Hijazi عذراء الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 47-53
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Tooth eruption is a more general process, however, which includes certain posteruptive toothmovements. There are two fundamental requirements for both tooth eruption to occur:(1) Require soft tissue, intervening between tooth structure and alveolar bone, which plays an important role inregulating the remodeling of adjacent tissues.(2) Require bone turnover that is temporally and spatially regulated to facilitate specific translocations of teeththrough alveolar boneThese amniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful forhuman application and recently it used in many medical branches. CD34 is an endothelial marker that is extensivelyused in immunohistochemistry and most vascular endothelial cells. Expression of the stem cell antigen CD34 is adefining hallmark of hemopoietic stem cells and progenitors. This study aimed to study the expression of CD34 bydental cells involved in tooth eruption after administration of amniotic stem cellMaterials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: Immunohistochemical result revealed positive expression of CD34 in pulp (Vascular, Paravascular),Mesenchymal cell and in the Dental sac of different groups. Coincidence test of expression marker CD34 in variousstudied group shows that Chorion application affected on CD34 expression in pulp while Amniotic fluid affected ondental sac.Conclusion Immunohistochemical study of expression marker CD34 in various studied groups show that chorionapplication affected on CD34 in pulp .While amniotic fluid affected on dental follicle


Article
Enhancement of tooth eruption by using amniotic stem cells (Immunohistochemical study of VEGF marker)

Authors: Athraa Y. Al- Hijazi عذراء الحجازي --- Lubna K. Jassim لبنى جاسم
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 80-88
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Tooth eruption is a localized process in the jaws which exhibits precise timing and bilateral symmetry.Develop within the jaws and their eruption is a complex infancy process during which they move through bone totheir functional positions within the oral cavity. For species with more than one set of teeth, eruption of the second setalso accomplishes. The key to the successful clinical management of tooth eruption consists of understanding thatthis process consists largely of the local regulation of alveolar bone metabolism to produce bone resorption in thedirection of eruption and shift and formation of bone at the opposite side.The amniotic sac contains a considerablequantity of stem cells. These amniotic stem cells are able to differentiate into various tissues, which used in many field.Vascular endothelial growth factor (VEGF) is an important angiogenic factor reported to induce migration andproliferation of endothelial cells, enhance vascular permeability, and modulate thrombogenicity. VEGF expression incultured cells (smooth muscle cells, macrophages, endothelial cells) is controlled by growth factors and cytokines.The aim of this study was to study the administration of cell molecules of (Chorion, Amnion and Amniotic fluid)around developing mouse tooth and studying the expression of VEGF marker.Materials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: VEGF marker localized and identified in 3 areas; pulp, P.D.L, and Bone. In pulp. The mean value of positiveVEGF expression showed to be highest in Amnion group in comparison to the other studied groups. The marginalmean value of all periods reported to be highest in Amnion groups followed by Chorion group. The period 10 dayshowed highest marginal means value for positive VEGF expression for all groups. In P.D.L. area Amniotic fluid recordsthe highest mean and marginal mean value specifically at day-10 in comparison to other studied groups. In Bonearea Amniotic fluid records the highest mean and marginal mean value among the studied groups followed byChorion group. Period 7-day and 10-day shows high mean value for VEGF expression. Coincidence test for VEGFmarker illustrates to be affected by Amniotic fluid application in P.D.L. and in bone area while Amnion and Chorionapplication showed to be concerned with pulp.Conclusion. It reported that amniotic fluid application affected on expression of VEGF in P.D.L and bone whileamnion and chorion showed to affect on expression of VEGF in pulp.The present study highlighted on clinical andresearcher application of Amniotic fluid and Chorion for supplement of stem cell in dental tissue engineering or evenin other body tissues.

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