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Article
Evaluation of the Antimicrobial Effect of Ethanolic Extracts of Sage and Rue as a Root Canal Irrigants (An In Vivo Study).

Author: Maha M Al –Bazzaz
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 18 Pages: 310-316
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate the antimicrobial effect of the ethanolic extracts of Ruta graveolens (Rue) and Sal-via officinalis (Sage) in a concentration of 0.2% and compare the results with the same concentration of chlorhexidine 0.2% (CHX) and normal saline on root canal bacteria using the above plant extracts as an irrigating solutions clinically. Materials and Methods: Thirty five uniradicular teeth with necrotic pulps were chosen. The patients were divided randomly into four groups, 10 patients for groups I, II and III and 5 patients for group IV. Using 0.2% ethanolic extract of Sage, 0.2% ethanolic extract of Rue, 0.2% chlorhexidine gluconate (CHX) and normal saline, respectively. Samples were obtained from the canal at the beginning of the first and second appointments, at the end of the second appoint-ment and at the beginning of the third appointment using wet sterile paper points. Results: The results revealed that 0.2% of the ethanolic extract of both Sage and Rue have a significant antimicrobial effect when used clinically as an endodontic irrigant, and was significantly not different from 0.2% chlorhex-idine gluconate (CHX) and significantly different from normal saline. Conclusions: Rue and Sage demonstrated antimicrobial effects on the root canal bacteria (both aerobic and anaerobic) used as en-dodontic irrigants compared with CHX.


Article
Evaluation of Antimicrobial Activity of Mineral Trioxide Aggregate (MTA)

Authors: Ennas Y Shehab --- Maha M Al–Bazzaz --- Mahmoud Y Taha
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2009 Volume: 9 Issue: 14 Pages: 216-219
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate the antimicrobial activity of MTA against selected microorganisms compared with a
widely used root end filling materials. Materials and methods: Fifteen mm discs of MTA, GIC and
Amalgam were prepared and three types of microorganisms; two bacteria and one fungus, were grown
in 4 ml of brain heart infusion broth for 18 hr. Then 0.5 ml of each growth was spread over selected
media (three plates for each sample) and the discs were applied on the agar, incubated for 24–48 hr and
the zone of inhibition was measured. Results: Amalgam did not demonstrate any antimicrobial activity,
whereas MTA showed antimicrobial effect against all tested microorganisms and was highly significant.
GIC showed antibacterial activity comparable to MTA but failed to produce antifungal effect.
Conclusions: MTA demonstrated antibacterial and antifungal effect, while GIC showed only antibacterial
activity, whereas Amalgam did not show any activity.

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Article
An Evaluation of the Antibacterial Effects of Rue and Sage Extracts

Authors: Siba M Al–Haliem --- Maha M Al–Bazzaz --- Ghada Y Abdul– Rahman
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 15 Pages: 55-61
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To investigate the antimicrobial effect of different concentrations of ethanolic and aqueous extracts of Sage and Rue (0.5%, 0.05%, 0.005%) on bacterial samples. They were iso-lated from root canal system in vitro, and the results were compared with that of chlorhexidine glu-conate (0.2%) as a positive control and normal saline as a negative control. Materials and Methods: Eighteen hours cultured bacterial samples of the root canal were used. To each 4ml of Brain Heart Infusion Broth (BHI), 0.1 ml of both bacterial sample and the examined solution Sage, Rue and chlorhexidine were added. Cultures were incubated at 37°C for 48 hours. The absorbance val-ues of the cultures were determined by the turbidity method using spectrophotometer at (550 nm). Re-sults: The results showed that both ethanolic and aqueous extracts of Sage and Rue have anti-bacterial effects in all concentrations with a significant variance compared with chlorhexidine against root canal microorganisms, while normal saline failed to show any antimicrobial activity. The results also showed that the ethanolic extracts of both plants have a higher antibacterial activity than the aqueous extracts. Conclusions: Rue and Sage demonstrated antibacterial effects on the root canal microorganisms (both aerobic and anaerobic) when compared with chlorhexidine.

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