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Article
Frequency of Anti Lactoferrin Antibodies in Patients With Systemic Lupus Erythematosus and Rheumatoid Arthriti

Authors: Nahla Ghanim Abdul–Majeed --- Mahmood Raheem Al-Rubaye --- Najeha Ahmed Ameen --- leen Khalooq Mustafa Kamil
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 4 Pages: 571-577
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: Lactoferrin is a multifunctional iron-binding protein present in several mucosal secretions as well as in secondary granules of polymorphonuclear leukocytes.Anti-Lactoferrin antibodies, which belong to antineutrophil cytoplasmic antibodies have been described in several immunomediated diseases, including Systemic Lupus Erythematosus and in patients with Rheumatoid Arthritis and others, with conflicting results regarding either their prevalence or clinical associations.OBJECTIVE:Detection of anti-Lactoferrin antibodies in Systemic Lupus Erythematosis and Rheumatoid Arthritis patients and studying its association to disease activity in comparison to healthy controls.PATIENT& METHODS :The study involved 74 Systemic Lupus Erythematosis patients, 40 Rheumatoid Arthritis patients who were referred to Immunological Department in Teaching laboratory Medical City during period of (1st of January – 31st of June) 2011 and 30 apparently healthy individual. Antinuclear antibody, complement C1q, rheumatoid factor and lactoferrin antibody were detected by enzyme-linked immunosorbent assay technique While double stranded DNA was detected by indirect immunofluorescent technique and complements (C3, C4) by single radial immune diffusion.RESULTS: Anti-lactoferrin Ab was detected in 14(18.9%) SLE patients, 4 (8.9%) Rheumatoid arthritis patients. Both C3&C4 levels were decreased significantly in Systemic lupus with positive anti lactoferrin Ab level in comparison to healthy controls( p value 0.024). In this regard Circulating immune complex was positive in 38(51.3%) systemic lupus patients only 12(31.5%) had positive lactoferrin antibody level, (p value 0.004).double stranded DNA was detected in 41(55.4%) Systemic lupus patients only 7(17.0%) of them had positive lactoferrin Ab level. Anti -Lactoferrin Abs showed neither a significant correlation with Rheumatoid factor IgG& IgM ( P value 0.159 ) nor with rheumatoid factor IgA ( P value 0. 857). CONCLUSION: Anti-lactoferrin antibodies could be detected in patients with Rheumatoid Arthritis and more often in patients with Systemic Lupus with significant correlation to decrease complements levels in comparison to healthy control.


Article
Anti Cathepsin Antibody In Systemic Lupus Erythematosus

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Abstract

Abstract:Antineutrophil cytoplasmic autoantibodies (ANCA) were found in patients withsystemic lupus erythematosus (SLE). Cathepsin G was the major target antigen. However,some ANCA-positive sera did not recognize either of them.The present study was toinvestigate the unknown target antigens of ANCA (Cathepsin G) in patients with SLE and itsrelation to the activity of the disease (ds DNA ),C1q ,C3and C4.Seventy four SLE patients referred to immunological department in teachinglaboratory medical city during period of (1st of march–31st of May) 2011 and 30 apparentlyhealthy individual as a control group were subjected to Antinuclear antibody (ANA),Cathepsin G and C1q were detected by enzyme-linked immunosorbent assay (ELISA)technique.While dsDNA was detected by indirect immunofluorescent (IIF) technique and C3,C4 by single radial immunodiffusion (SRID).The age of SLE patients ranged between (4-71) years with mean age of 32.4. Themean C1q and Cathepsin G levels were 30.14 and 9.7 respectively with significant differenceif compare it with the healthy control group. A significant result found between mean age ofSLE patients and Cathepsin Ab of a P value 0.007.The Cathepsin G antibody was found in 26 (35%) of SLE patients with a significant Pvalue (0.0001).The Cathepsin Ab were detected in 18 (43.9%) of a patients with positive ds DNAwith no significant P value 0.078. while C1q Ab were detected in 38 (51.4%) of SLE patientsand only 18 (47.4%) from them with Cathepsin Ab with statistically significant P value 0.024.Cathepsin Ab found in 19 (42.2%) of SLE patients with low level of C3 and only 6 (21.4%)of normal level of C3 with no significant P value0.076.This study showed 54(73.0%) of SLE patients had low level of C4 with no significantP value (0.15) and the Cathepsin Ab detected in 22 (40.7%) of them with no statisticallysignificant P value 0.097.A statistically significant result were found for ANA, C1q,C3,C4 and Cathepsin Abif we compare the result of SLE patients with control group, while The Cathepsin G antibodywas found statistically significant in SLE patients ,with significant relation to C1q,on otherhand no relation found with the activity of the disease (ds DNA ),C3 and C4 levels.Key Word: SLE, Cathepsin G, ds DNA, C1q, C3, C4

الخلاصة:لدى المرضى (pANCA) P وجدت الاجسام المضادة الضادة لسایتوبلازم كریات الدم البیض العدلة نوعمع ANCA المصابین بداء الذئب ألاحمراري وكان الكثبسین ج ھو المستضد المستھدف، وان نسبة علاقة مضادمستضاداتھا تحت الدراسة.الغرض من ھذه الدراسة ھو لمعرفة العلاقة بین مضاد الكثبسین ج وفعالیة المرض المتمثلة بالشریط المضاعف.(C3,C ومستوى المتمم الثالث والرابع ( 4 (dsDNA) للحامضالنووي الدیوكسي الرایبوزيخلال الفترة من الأول من آذار إلى 31 أیارعام 2011 تم تحویل 74 مریض مصابین بداء الذئب ألاحمراري إلىقسم المناعة في المختبرات التعلیمیة في دائرة مدینة الطب وتم اخذ عینات منھم مع 25 عینة من أشخاص أصحاءباستعمال تقنیة c1q ومضاد الكثبسین ج و ANA كمجموعة سیطرة، وتم أجراء فحوصات الاجسام المضادة الضادة للنواة.SRID بواسطة C و 4 C و 3 IIF بواسطة dsDNA الامتزاز المناعي المرتبط بالإنزیم (الالیزا)، وتم فحصال

Keywords

Key Word: SLE --- Cathepsin G --- ds DNA --- C1q --- C3 --- C4.

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