research centers


Search results: Found 3

Listing 1 - 3 of 3
Sort by

Article
EFFECT OF HELIUM-NEON LASER ON THE LYMPHOCYTE CELLS AND THEIR DNA

Authors: Siham S. Abdullah سهام صباح عبد الله --- Jenan H. Taha جنان حسن طه --- Majid H. Ahmed ماجد حميد احمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 3 Pages: 275-282
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Laser light is widely used for a wide range of medical applications. He-Ne laser application in medicine as in any type of laser is based on the interaction of laser light with the biological system.Objective:To show the effect of helium-neon (He-Ne) laser (632.8 nm) irradiation on human lymphocyte blood cells and their DNA.Methods:This study involved 72 blood samples, taken from apparently healthy volunteers. The samples were divided into two groups; the 1st group consisted of 27 samples were processed only for lymphocyte blood cells separation, while the 2nd group, which consisted of 45 samples were employed to evaluate the influence of He-Ne laser irradiation on the extracted DNA from the lymphocyte blood cells.Results:At the used doses of He-Ne laser (18, 35, 52.5, and 69 J/m²), a significant difference was found (P < 0.05) in survival percentage of lymphocyte cells (99.8, 99.74, 99.68, and 99.59) in comparison with those cells untreated with He-Ne laser irradiation. Immediately after He-Ne laser irradiation alone, the following doses (18, 35, and 69 J/m²) were applied on the extracted DNA, the DNA demonstrated a significant damaging where the fraction of DNA survival percentage was (88.6, 87.7, 86.1) respectively, with significant difference (P < 0.05) between the DNA survival before and after He-Ne laser irradiation.Conclusion:The percentage of lymphocytes survival is decreasing with increasing dose of He-Ne laser and longer exposure time where time exposure (2.5, 5, 7.5, and 10 s). He-Ne laser irradiation causes a significant degree of DNA damaging independent on the irradiation doses.Keywords:Lymphocyte cells, He-Ne laser irradiation, DNA. Citation:Abdullah SS, Taha JH, Ahmed MH. Effect of Helium-Neon Laser on the Lymphocyte Cells and their DNA. Iraqi JMS. 2017; Vol. 15(3): 275-282. doi: 10.22578/IJMS.15.3.9


Article
INFLUENCE OF CIGARETTE SMOKING ON SEMINAL PLASMA SOLUBLE FAS AS A MARKER OF GERM CELL APOPTOSIS

Authors: Majid H Ahmed ماجد حميد احمد --- Isra'a F Ja'afar اسراء فائق جعفر --- Anam R Al-Salihi انعم رشيد الصالحي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 1 Pages: 12-17
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Male infertility constitutes about 50% of overall causes of infertility and apoptosis is known to have an essential role in the control of germ cell number in testis. Cigarette smoking is common in males at reproductive age. Studying the influence of smoking on apoptosis in male genital tract and its influence on fertility helps in the management of infertility in smokers.Objectives:To assess the influence of cigarette smoking on seminal plasma (SP) soluble Fas (sFas) and the correlation between SP sFas and serum sex steroidal hormones and conventional semen parameters in males of infertile couples.Methods:Seventy male partners of infertile couples (30 smokers and 40 non-smokers) were enrolled in this study. A subject was considered smoker if he had history of smoking of more 10 cigarettes per day for at least one year. Semen analysis was done according to World Health Organization (WHO) 2010. Specific kits were used for the measurement of SP sFas and serum testosterone and estradiol (E2).Results:In smokers' group, SP sFas was significantly (p <0.05) negatively correlated with age and sperm motility and positively correlated with immotile sperm and round cell number.Conclusion:Germ cell apoptosis in smoker males of infertile couples is interrelated with sperm motility.Keywords:Male infertility, Smoking, Apoptosis, Seminal plasma sFas.


Article
2.ASSESSMENT OF SERUM ZINC LEVEL IN PATIENTS WITH ATOPIC DERMATITIS

Loading...
Loading...
Abstract

Background:Atopic dermatitis (AD) is a common inflammatory skin disease with a chronic relapsed-remitting course with manifestations started at early childhood. AD is two types; extrinsic and intrinsic. Zinc has a crucial role in the immune system functions and antioxidant mechanisms, it also serves in the metabolism and cell growth through signaling several enzymes.Objective:To assess serum zinc level in patients with childhood AD.Methods:A case-controlled study was conducted in Dermatology outpatient’s clinic in Al-Imamein Al-Kadhimein Medical City, from the period of September 2016- June 2017. Twenty patients with AD were enrolled in this study. AD severity was graded using the Scoring Atopic Dermatitis Index (SCORAD). The control group consisted of age-matched and sex-matched twenty healthy children were taken with weight above 80%. Fasting blood samples were taken from patients and controls between 8 AM and 10 AM.Results:Of the twenty patients of AD, 13 (65%) were males and 7 (35%) were females. Their mean age was 4.58±3.13. Regarding the control group 9 (45%) were males and 11 (55%) were females, their mean age was 4.29±2.46. No statistical difference in serum zinc level between AD patients and control groups. Concerning zinc level in males and females, no statistical differences was found in AD patients, but highly significant difference was found in control group. Serum zinc level was highly significantly low in patients with moderate AD than in those with mild AD. There was negative correlation between serum zinc level and severity of AD.Conclusion:There is a negative correlation between serum zinc level and severity of AD, also there is a gender variation in serum zinc level in normal children.Keywords:Serum zinc level, atopic dermatitisCitation: Farhood IG, Ahmed MA, Al-Bandar RT, Farhood RG. Assessment of serum zinc level in patients with atopic dermatitis. Iraqi JMS. 2019; 17(2): 103-107. doi: 10.22578/IJMS.17.2.2

Listing 1 - 3 of 3
Sort by
Narrow your search

Resource type

article (3)


Language

English (3)


Year
From To Submit

2019 (1)

2017 (1)

2013 (1)