research centers


Search results: Found 4

Listing 1 - 4 of 4
Sort by

Article
The Relationship Between IL-10 and Dislipidemia in Type 2 Diabetics

Author: Majid Kadhum Hussain
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2011 Volume: 22 Issue: 4 Pages: 202-213
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

The current study was designed to estimate the differences in serum interleukin–10 (IL-10) levels in patients with type II diabetes and control group also to demonstrate whether a relationship was indicated among IL-10 and the changes in the levels of lipid and lipoproteins (dyslipidemia) in the same patients. To achieve this aim, 49 diabetic patients (21 females and 28 males) at ages of 47.23±14.23 y were enrolled. To compare the results, 30 healthy individuals (17 females & 13 males) of ages 39.35± 3.89 y were also included as a control group. Serum IL-10 levels were measured in the patients & the control group by an enzyme linked immunosorbent assay (ELISA). Fasting blood glucose level (FBGL) was estimated by the glucose-oxidase method. Total cholesterol, triglyceride and HDL were measured by colorimetric method. The results revealed significantly (P<0.001) decreased of serum IL-10 levels in diabetic patients when compared with those of the control group. Total cholesterol, triglycerides, LDL and VLDL values exhibited significantly (P<0.005-0.0001) elevation in the diabetic patients when compared with those of control group.While HDL level significantly (P<0.0001) decreased in diabetic patients in comparison with those of control group.The linear regression analysis revealed a significant negative correlation for cholesterol (r =-0.45, P<0.001), triglycerides (r =-0.37, P<0.05), LDL (r=-0.30, P<0.05) & VLDL(r=-0.37, P<0.05) level and positive correlation HDL (r=0.49, P<0.001) levels with the IL-10 values in the diabetic patients. Such correlation was not observed for the level of cholesterol, HDL and LDL in control group. Serum IL-10, Total cholesterol, triglycerides &HDL levels were shown to be significantly (r=-0.51, P<0.001, r=0.65, P<0.001, r=0.58, P<0.001, r=-0.49, P<0.05), correlated with FBGL in the diabetic patients but not in control group. In conclusion, low level of IL-10 could be involved in pathogenesis of type 2 diabetes and there is association between IL-10 and dyslipidemia in these patients.

تم تصميم الدراسة الحالية لتقديرمستوى الانترلوكين-10 (IL-10) في مرضى السكري النوع الثاني ,وايضا لاثبات فيما اذا كان هناك علاقة بين IL-10 و التغيرات في مستويات الدهون والبروتينات الدهنية لدى نفس المرضى. لتحقيق هذا الغرض تم دراسة 49 من مرضى السكري (12اناث و28ذكور) معدل اعمارهم 23-60) 47.23±14.23 ) لمقارنة النتائج تضمنت الدراسة 30 شخصا من الاصحاء (17اناث و 13ذكور) معدل اعمارهم39.35± 3.89 (55-27) وصفت كمجموعة سيطرة. قيست مستويات IL-10 بطريقة التقدير المناعي الممتز والمرتبط إنزيميا (ELISA) بينما قدر مستوى السكرالصيامي بالدم ( (FBGLبطريقة Glucose-oxidase وتم تقدير تراكيز الكوليسترول والكليسيردات الثلاثية والبروتينات الدهنية, بالطرق اللونية. وكشفت النتائج انخفاضا معنويا (P<0.001) في مستوى IL-10 لدى مرضى السكري عند مقارنته مع امثاله في الاشخاص الاصحاء بينما لوحظت زيادة معنوية (P<0.05-0.001) في مستوى الكوليسترول والكليسيردات الثلاثية والبروتينات الدهنية (LDL, VLDL).فيما لوحظ انخفاضا معنويا (P<0.0001) في مستوى HDL لدى مرضى السكري النوع الثاني بمقارنتها مع الاشخاص الاصحاء . اظهر تحليل الارتباط الخطي علاقات ارتباطية معنوية سالبة بين مستويات الكوليسترول,P<0.001) (r=-0.45والكليسيردات الثلاثية (r=-0.37 P<0.05), (r=-0.30, P<0.05) LDL و VLDL (r=- 0.37 P<0.05) وموجبة HDL 0.49,P<0.001) r =) مع مستوى IL-10 في مرضى السكري لم تلاحظ مثل هذه العلاقة لمستوى الكوليسترول وHDL وLDL عند الاصحاء. كما ظهرت علاقات ارتباطية معنوية سالبة لمستويات IL-10 (r=-0.51,P<0.001) و(r=-0.49, P<0.001) HDL وموجبة للكوليسترول (r=0.65,P<0.001) والكليسيردات الثلاثية (r =0.58, P<0.001) مع FBGL في المصابين بالمرض عند مقارنتهم بالاشخاص الاصحاء .بالنتيجة فان انخفاض مستوى IL-10 قد يساهم في الاصابة بداء السكري ومن جهة اخرى تشير النتائج الى العلاقة الواضحة بين مستويات الكوليسترول والكليسيردات الثلاثية و البروتينات الدهنية مع مستوى IL-10 لدى مرضى السكري النوع الثاني.

Keywords


Article
Association of Adiponectin Gene Promoter Polymprphism (rs266729) With Ischemic Heart Diseases

Authors: Maha Radhi Abass --- Majid Kadhum Hussain --- Zuhair Mohammed Ali Jeddoa
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2018 Volume: 11 Issue: 1 Pages: 3889-3893
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

background: Ischemic Heart Disease is a group of diseases of the blood vessels supplying the heart muscle. Adiponectin is a protein secreted by adipocyte with insulin-sensitizing, Anti-inflammatory and anti-atherogenic properties. Several studies have shown that polymorphisms within the adiponectin gene can be associated with Ischemic heart disease. Aim: The aim of the present study was to evaluate the impact of (rs266729) SNP in the promoter region of the ADIPOQ gene on the occurrence of Ischemic heart disease (IHD).Methods: The study included 150 patients with IHD randomly selected based on World Health Organization (WHO) guideline and 150 as controls group. DNA was extracted from blood and genotyped by PCR-RFLP by using (Hha1) enzyme. Result: The frequency of G allele of rs266729 (C/G) polymorphism was significantly (p=0.0001) in IHD (19.6%) compared with control (13.3%). The homozygous genotype (GG) significantly (0R=1.71, CI 95%=0.65-4.96, P= 0.0001) increased the risk of Ischemic Heart Disease compared with wild type (CC) after adjustment age, sex, and BMI, furthermore the heterozygous (CG) genotype significantly (0R=1.61, CI 95%=0.96-2.87, P= 0.0001) raised the risk of Ischemic Heart Disease.Conclusion: Adiponectin gene polymorphism rs266729 is involved in the pathogenesis Ischemic heart disease.


Article
Association between Resisten Gene Polymorphism (420C/G) and Lipid profile in Ischemic Heart Diseases

Authors: Asraa Ali Kadhum --- Majid Kadhum Hussain --- Riyadh. Dh. Al-zubaidi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2018 Volume: 11 Issue: 1 Pages: 3937-3942
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

background: (resistin is considered as a pro-inflammatory molecule and plays a role in the inflammatory response that lead to atherosclerosis)Aim: To evaluate the risk of resisten gene polymorphism (420C/G) in the development of Ischemic Heart Disease. To verify the relationship of the investigated SNPs with the metabolic changes related to (IHD), in particular, serum lipid profile.Methods: A case control study was performed at which 150 patients with IHD and 150 healthy individuals. Genotyping for SNP 420C>G in the resistin gene was performed by the polymerase chain reaction –restriction fragment length polymorphism method. Lipid profile were measured.Results: The genotype and allele frequencies of resistIn gene polymorphism in IHD and control persons were examined under the co-dominant, dominant and recessive models with the use of multi nominal logistic regression analysis. Neither genotype distribution nor the minor allele frequency showed significant changes among the comparison of the of IHD patients with the control group. The frequency of the G allele of 420(CG) polymorphism was significantly higher in ischemic heart diseases (IHD). There are significant increases in the level of LDL, triglyceride, VLDL, BMI and a significant decrease in the level of cholesterol in the group of patients with the GG+CG genotypes when they were compared with those of the CC genotype. It shows significant differences in BMI, Age, Cholesterol, LDL, and HDL in the group of IHD, and no significant difference was seen in VLDL and sex.Conclusion: The −420C>G SNP of resistin gene is not associated with ischemic heart disease in the population of Kerbala and Najaf. The G allele is seemed to increase serum lipid concentrations so it could be considered as an atherosclerotic parameter.


Article
The Relevance of Glycosylated Hemoglobin with Oxidative Stress in Insulin Resistant Type 2 Diabetes Mellitus

Authors: Majid Kadhum Hussain --- Dhafera Jaffer Abed-Ali --- Dhafera Jaffer Abed-Ali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2012 Volume: 5 no 1 Issue: 11 Pages: 1334-1342
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

The present study was conducted to evaluate the relevance of glycosylated hemoglobin with oxidative stress in insulin resistant type 2 diabetes mellitus. To achieve this aim, 93 type 2 diabetic patients of ages 38-84 years were recruited. In addition, 19 apparently healthy individuals with ages 30-60 years, were enrolled as a control group.The concentration of fasting blood glucose (FBG), triglyceride (TG), insulin, glycosylated hemoglobin (HbA1c), malodialdehyde (MDA) and glutathione-S-transferase (GST) were measured in sera of patient and the control groups. Fasting blood glucose and triglyceride levels were estimated by spectrophotometeric methods using enzymatic procedures. Insulin level was estimated by enzyme linked immunosorbant assay (ELISA) method. HbA1c level was determined by an ion exchange chromatographic method, while MDA and GST levels were measured by spectrophotometeric procedures. Insulin resistance was evaluated by four methods. They include homeostatic model assessment (HOMA), quantitative insulin check index (QUIKI), McAulye (McA), and fastinge insulin (FI) methods. Insulin resistance was found in 79 (84.9%), 63 (73.4%), 52 (55.9%) and 35 (37%) out of the 93 diabetic patients by HOMA, QUICKi, McA and FI methods respectively. Type 2 insulin resistant (79) diabetic patients (IRP) that obtained through the HOMA method were assessed for the HbA1c, MDA and GST levels in comparison to the insulin sensitive patients (ISP) as well as to the control group. The analysis of the data revealed a significant increase (p<0.001) of HbA1c levels in the IRP group when compared with those ofThe Relevance of Glycosylated Hemoglobin….. Majid Kadhum Hussainkerbala J. Med. Vol.5, No.1, Dec, 2012Pagethe control group, while the insulin sensitive group (ISP) couldn’t show significant variation when compared with those of the IRP. The data of MDA failed to give significant variation. However, a significant elevation of GST concentration were observed in the IRP group with respect to those of the control group (p<0.005). On the other hand significant variations could not be obtained among the ISP and IRP. These data suggested that oxidative stress changes are independent on insulin resistance in type 2 diabetes mellitus

Keywords

Listing 1 - 4 of 4
Sort by
Narrow your search

Resource type

article (4)


Language

English (3)

Arabic and English (1)


Year
From To Submit

2018 (2)

2012 (1)

2011 (1)