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Article
Antibacterial effects of mineral trioxide aggregate and Biodentine TM after the addition of different concentrations of black seed aqueous solutions

Authors: Linz A. Shalan --- Majida K. Al-Hashimi ماجدة الهاشمي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 1 Pages: 48-53
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Mineral Trioxide Aggregate (MTA) and BiodentineTM cements are new materials with numerous excitingclinical applications. Both have appreciable properties which include good physical properties and the ability tostimulate tissue regeneration as well as good antibacterial effects. The aim of this study was to investigate andcompare the antibacterial effects of MTA and BiodentineTM, when they were mixed with different concentrations ofaqueous solutions of Black Seed extract, against Enterococcus faecalis.Materials and methods: MTA and BiodentineTMwere prepared according to the manufacturer’s instructions. Themethod of Mawlood was followed to prepare the Black Seed aqueous solution. Agar diffusion method on Brain HeartInfusion agar was employed.Twenty, 9 cm diameter, petri-plates with 25 ml of Muller Hinton agar media wereprepared. A sterile spreader was used to inoculate the microorganisms. With a micropipette 0.1 ml of theEnterococcus faecalis suspension was added to the surface of the plates. Within 15 minutes, after inoculation of theplates, 4 cavities, each one measuring 5 mm in diameter and 4 mm in depth, were made in each agar plate. A totalof 20 agar plates were divided into 2 groups consisted of 10 plates each; Group A: each plate contained 4 wellsfilled with MTA alone and MTA mixed with 10%, 30% and 50% of Black Seed aqueous solutions respectively.Group B:each plate contained 4 wells filled with BiodentineTMalone and BiodentineTMmixed with 10%, 30% and 50% of BlackSeed aqueous solutionsrespectively. Next day after incubation, the agar plates were examined for bacterialinhibition zones. With a scientific ruler the diameter of the antibacterial inhibition zones were measured. The datawere recorded and statistically analyzed, by the ANOVA and the Student's t-test.Results: Both cements had antibacterial effects, which were increased with the addition of the aqueous solutions ofBlack Seed extract. The increase in the diameter of Enterococcus faecalis inhibition zones was directly proportionalwith the increase in the concentration of the added Black Seed aqueous extract.Conclusion: Adding aqueous solutions of Black Seed extract to both MTA and Biodentine™ increased their potentialto inhibit the growth of Enterococcus faecalis


Article
The influence of instrument application frequency on the apical extrusion of debris using rotary ProTaper, hand ProTaper and hybrid technique (An in vitro study)

Authors: Majida K. Al-Hashimi ماجدة الهاشمي --- Zaid K. Al-Doory زيد الدوري
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 4 Pages: 34-39
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Various kinds of hand-held or rotary instruments and techniques are used for mechanical preparationof the canal during root canal treatments. These instruments and techniques may push debris out of the canalswhich may induce inflammation within the periapical area; therefore, instrumentation technique that causes lessextrusion of debris is more desirable. The aim of this study was to compare the effect of instrument applicationfrequency on the amount of apically extruded debris.Materials and Methods: One hundred and twenty extracted human teeth were used in this study; all teeth wereshortened to a length of 15 mm. Each experimented root was mounted on a centrifuge tube that forced through aprecut hole in a rubber stopper of a glass vial. The roots were divided randomly into 3 groups, each group contained40 roots. Group R: prepared by rotary ProTaper, Group H: prepared by hand ProTaper, Group S: prepared by Hybridtechnique. Each group was further subdivided in to five subgroups (A, B, C, D, and E). Debris extruded from apicalforamen was collected in a centrifuge tube containing 0.5 milliliter of distilled water. Each empty centrifuge tube wasweighed before preparation by 0.0001g. sensitive weighing machine. Then at the end of canal preparation, thesecentrifuge tubes were completely dried using an incubator at 68 C° for two days and weighed again. The differencebetween the weights of tubes in two stages represented the weight of debris extruded from apical foramen duringinstrumentation.Results: No significant difference recorded for the mean weight of apically extruded debris regarding the instrumentapplication frequency within the same instrumentation technique; but there was a high significant difference for thesubgroups (A, B, C, and D) and a significant difference for subgroup E, regarding the effect of instrumentationtechniques on amount of apical extrusion of debris among tested groups.Conclusion: The hand ProTaper extruded smaller amounts of apical debris than the rotary ProTaper and largeramounts than Hybrid technique


Article
The effect of different acidic environments on the apical microleakage of different obturation techniques (An in vitro study)

Authors: Areej R. Ibrahim اريج ابراهيم --- Majida K. Al-Hashimi ماجدة الهاشمي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 18-24
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Pulpal and apical inflammation or infection decreases tissue pH in the region surrounding the involvedtooth which might affect the sealing ability of different obturation systems. This study evaluate the apicalmicroleakage of three obturation techniques (lateral condensation of Gutta-percha/AH 26, Soft-Core guttapercha/AH 26 and lateral condensation of Resilon/Real Seal SE), when exposed to 7.3 6.5, 6.0 and 5.5 pH values.Materials and method: One hundred and thirty two roots of freshly extracted teeth were selected. Teeth weredecoronated, working length was established and the roots were instrumented using a crown down technique withProTaper rotary files (SX-F3). The specimens were divided into three groups of 44 samples each. Group A: obturatedusing lateral condensation of gutta percha and AH 26. Group B: obturated using soft-core and AH 26. Group C:obturated using lateral condensation of Resilon and Real Seal SE. Each group was further subdivided into foursubgroups, 10 samples each, which were exposed to pH values of 7.2, 6.5, 6.0 and 5.5 respectively. Microleakagewas evaluated by longitudinal sectioning, and measurement of liner dye penetration.Results: There was a non significant difference within each group regarding the different pH media. Both Soft-Coreand Resilon showed less apical microleakage than lateral condensation of gutta percha with a highly significantdifference in all the tested acidic media.Conclusion: Resilon/Real Seal SE subgroups showed the least apical microleakage, however, it didn’t provide thecomplete sealing claimed by the manufacturer


Article
Shear bond strength of endodontic sealers to dentin with and without smear layer and gutta percha (An in vitro study)

Authors: Rana Kadhim Hasan رنا كاظم حسن --- Majida K. Al-Hashimi ماجدة الهاشمي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 4 Pages: 86-89
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The bond strength of root canal sealers to dentin and gutta-percha seems to be an important propertyfor maintaining the stability of root canal filling, which potentially influences both leakage and root strength. Theobjective of this, in vitro, study was to evaluate the shear bond strength of three different endodontic sealers (Gutta-Flow, AH Plus, Apexit Plus) to dentin, in the presence and absence of the smear layer and gutta percha.Material and Methods: After slicing off the occlusal 2mm of 60 extracted human maxillary premolar teeth, theexposed dentin served as the tested surfaces; the teeth were fixed with cold cure acrylic, and were divided into twogroups according to the smear layer presence, group A without smear layer, when dentin surfaces were irrigatedwith EDTA 17% followed by distilled water then subdivided into 3 subgroups according to the type of sealer used;group B when dentin surfaces were washed with distilled water only, then subdivided into 3 subgroups. Thirty samplesof gutta-percha were prepared and named as group C which was subdivided into 3 subgroups. Five mm longsection of polyethylene tubes were placed on the dentin or gutta percha surfaces and filled with freshly mixedsealer. After one week, all the samples were tested for shear bond strength by the Instron Universal Testing Machineat a cross head speed of 0.5 mm/min. The data was calculated in MPa and was statistically analyzedResult: There was a highly significant difference in the shear bond strength (P < 0.05) in comparison among the testedgroups, GuttaFlow showed non-significant difference in bond strength to dentin with and without smear layer, whileAH Plus and Apexit Plus showed a high significant difference.Conclusions: AHPlus showed the highest shear bond strength in all the tested samples, while GuttaFlow was the least.Additionally, AH Plus and Apexit Plus shear bond strengths were affected by the smear layer removal, whileGuttaFlow was not.

صیة مھمة للحفاظ على ثبات حشوة قناة الجذر الذي بدوره یؤثرعلى تسرب و قوة الجذر. Gutta-percha أن لقوة الرابطة لسدادات قناة الجذرالى عاج السن وو عاج السن بوجود اوعدم (AH Plus, Gutta-FlowApexit Plus,) كان الغرض من ھذه الدراسة تقییم قوة الترابط القصي بین ثلاثة انواع من السدادات اللبیة المختلفةبعد قطع 2ملمیتر من تاج 60 سن من أسنان الإنسان(الضواحك العلیا) . تم تثبیت الأسنان في مادة الاكریلیك ، و قسمت .Gutta-percha و ال Smear layer وجود طبقة ال.Smear layer الى مجموعتین وفقا لوجود طبقة ال(AP-) %17 یلیھ الماء المقطر وقسمت إلى 3مجموعات فرعیة وفقا لنوع السدادة المستعملة EDTA على سطح عاج السن مع غسل بمادة Smear layer المجموعة (أ) بدون.D, AH-D, GF-D. APD-S, AHD-S, GFD-S)( المجموعة (ب) غسل سطح عاج السن بالماء المقطر فقط ثم قسمت إلى 3 مجموعات فرعي.(AP-G, AH-G, GF-G) أعدت لتكون المجموعة (ج)وقسمت إلى 3 مجموعات فرعیة (Gutta-percha) ثلاثونعینة مناختبار قوة الرابط القصي تم بعد اسبوع . . Gutta-percha استعمل انبوب بطول 5 ملم من البولي اثیلین بسداده مخلوطة حدیثا ووضع على سطح عاج السن أو السجل فرق معنوي غیر ملحوظ في قوة الترابط مع عاج السن بوجود Gutta-Flow. تم حساب البیانات بوحدة المیغا باسكال و تم تحلیلھا إحصائیاً . أظھرت النتائج فرقا معنویا كبیراسجلوا فرقاً معنویاً عالیاً. Apexit Plus و AH Plus بینما Smear layer اوعدم وجود التأثرت Apexit plus و AH Plus الاقل بینھم . وقوة الربط القصي لل Gutta-Flow اظھر اعلى قوة ربط قصي في جمیع العینات بینما كان AH Plus : الاستنتاج النھائيفلم تتأثر.

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