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EFFECTS OF AFLATOXIN B1 ON SOME SKELETAL MUSCLE RESIDENT CELLS USING A NUCLEAR DIFFERENTIATING STAIN TECHNIQUE
تأثيرات الافلاتوكسين ب1 في بعض الخلايا القاطنة في العضلات الهيكلية باستخدام تقنيه صبغة تمايز الانويه

Author: May F. Al-Habib مي فاضل الحبيب
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2007 Volume: 5 Issue: 2 Pages: 71-77
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Aflatoxins are one of the toxigenic fungi that draw attention for researcher, they are a group of closely related mycotoxins that can contaminate food. The problem of using contaminated food with toxigenic fungi is still one of the most important stigmas in the field of nourishment of human and animals.Objectives:This study was designed to determine how Aflatoxin B1 contaminated food and feeding regimen might affect and induce specific changes in the muscle resident cells.Methods:Two groups of animals were studied one fed with Aflatoxin B1 contaminated food and the other fed with Aflatoxins free diet. Rats were fed daily with diet contaminated with the spore. The Extensor digitorum longus muscle was removed and cut into small pieces and prepared by the method of Torikata (1988). Semi thin sections were obtained and stained by a nuclear differentiation stain.Results:Animals treated with Aflatoxin B1 have shown a marked increase in body weight. Aflatoxin B1 showed pronounced effects on muscle nuclei and on the vascularity of skeletal muscle fibers.Conclusions:lt has been concluded that AFB1 have marked effects on the number of cells found in skeletal muscles.Keywords: Aflatoxins B1- Skeletal muscle- nuclear differentiating stain

خلفيه الدراسة : سموم الافلاتوكسين ب 1 هي واحده من سموم الفطريات التي جذبت انتباه العلماء للأبحاث وهذه السموم عبارة عن مجموعه مترابطة من سموم الفطريات. إن مشكله استخدام الاغذيه الملوثة بسموم الفطريات لا تزال واحده من أهم السمات في حقل التغذية للإنسان والحيوان.هدف الدراسة : صممت هذه الدراسة لقياس ومتابعه مدى تأثير استعمال أغذيه ملوثهبالافلاتوكسين ب1 في العضلات الهيكلية والتغيرات التي تحدثها في الخلايا القاطنة للعضلات الهيكلية.طرائق الدراسة : استخدمت في هذه الدراسة مجموعتين من الجرذان البيض، أعطيت المجموعة الأولى سموم الافلاتوكسين ب1 مع الغذاء والمجموعة الثانية وهي مجموعه السيطرة أعطيت غذاء بدون الافلاتوكسين.استأصلت العضلة الباسطة للأصابع وقطعت إلى قطع صغيره ثم تم تحضير الانسجه للحصول على مقاطع شبه خفيفة وصبغت بصبغه تمايز الانويه.النتائج:•هنالك ازدياد ملحوظ في وزن الجسم للحيوانات التي استعملت الغذاء الملوث بسموم الافلاتوكسين ب1.•انويه الخلايا العضلية الهيكلية أظهرت قله بالعدد مع قله بعدد ألاوعيه الدموية في الحيوانات المعالجة بغذاء الافلاتوكسين ب1.الاستنتاجات : لقد تم استنتاج وجود تأثيرات ملحوظة لسموم الافلاتوكسين على عدد الخلايا القاطنة في العضلات الهيكلية.مفتاح الكلمات : سموم الافلاتوكسين ب1 – العضلات الهيكلية – صبغه تمايز الانويه.


Article
AGE-RELATED CHANGES IN HUMAN SKIN: HISTOLOGICAL, MORPHOMETRIC AND IMMUNCYTOCHEMICAL STUDY USING S100

Authors: Salih S Kadhim صالح سلمان كاظم --- May F Al-Habib مي فاضل الحبيب
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 2 Pages: 111-118
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Aging has many effects on a person’s skin, from wrinkles and sags to increased risk of certain skin conditions, such as skin cancer. As people age, their skin begins to change due to environmental, genetic, nutrition and other factors.Objectives: Understand some of the changes that occur in aging skin including changes in the general morphological, histological and architectural arrangement, epidermal thickness, basement membrane and histochemical changes in melanocytes.Methods: Skin specimens were taken from the anterior abdominal wall of 30 human males at different ages. General histological preparation for paraffin blocks was performed and the blocks were sectioned at (5-6µ) and stained with H&E. S100 protein was used to demonstrate immunohistochemistry labeled melanocytes changes with age. Histometric measurement of epidermal thickness and basement membrane thickness, using eyepiece graticule was performed on these groups.Results: The young age group showed a uniform arrangement of cells in all stratums of the epidermis while the old age group showed diminished thickness of the epidermis. A significant difference between young- adult age group (A and B groups) and the old age group (C group) was recorded. The epithelial basement membrane thickness was increased with age significantly (P value ≤ 0.001). Melanocytes demonstration using S100 showed that these cells tend to be situated at the tips of rete pages, there number are generally low and didn't varied a lot between young and adult age groups. There was yet marked decline in the number of melanocytes in old age group.Conclusion: Aging as a process have a marked influences on skin morphology, thickness, cellularity and basement membrane.Key Words: Aging, skin, S100, morphometry and basement membrane

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