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Evaluation The Serum Total Protein in Patients with Diabetes Mellitus (Type I and Type II) and Study Genetic Level of Glutathione-S-Transferaseµ 1

Authors: Nisreen Jalal Mohammed --- Moaed Emran AL-Gazally --- Maysam Ali Ameen Awadh
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 625-631
Publisher: Babylon University جامعة بابل

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Abstract

Diabetes mellitus is a group of metabolic diseasesdue to defect in insulin secretion or action or both.Hyperglycemia in diabetes creates free radicals .These free radicals produce oxidative stress and thus debilitate the endogenous antioxidant defense system.If the amount of insulin available is insufficient, cells response badly to the effects of insulin or if the insulin itself is abnormal. The net effect is persistently elevating levels of blood glucose, low protein synthesis.The current study evaluate the biochemical changes in diabetes mellitus patients in using different medications and investigate the glutathione S-transeferase M1 gene deletion in different treatments. The present study was conducted on (75) diabetic patients, (25) of them were treated with insulin, (25) were using insulin and metformin and the last (25) were on metformin and glibinclimide .The study also included (25) apparently healthy subjects were taken as control group. The blood sampleswere collected from Merjan Teaching Hospital from November 2013 to April 2014.The total serum protein was measured by colorimetric method described by Gornall.LSD test showed that there was significant difference between insulin & metformin group and metformin &glibinclimide in total serum protein (p ≤0.05). Metformin &glibinclimide group was less mean difference with the control group. In the gene level of the study, genotyping of glutathione S-transferase mu 1gene by PCR were defined as GSTM1 and GSTM0 or deletion association to the present and absences of the guanine nucleotide in the gene sequence. There was statistically significant difference in the genotyping distribution and the frequency of GSTM0 among study groups were 44% for insulin and metformin group, 68% for metformin and glibinclimide, 44% insulin and 28% for control healthy group.there was significant decrease in serum total protein in diabetic patients. Genetic polymorphism of glutathione S-transferase mu 1gene may be considered as risk factor for both types of diabetes mellitus.

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