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Article
Evaluation of CD69 as a prognostic factor and its relation with other prognosticatos in a sample of Iraqi patients with chronic lymphocytic leukemia

Author: Maysem Mouayad Alwash
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 2 Pages: 69-74
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Chronic lymphocytic leukeamia (CLL) is clinically heterogenous disease with variable prognosis . It is important to take into consideration the prognosis of presenting with CLL before starting suitable therapeutic option. Different studies proved that leukemic CLL cells have the surface membrane phenotype of activated and antigen experienced B lymphocytes with the overexpression of the activation markers such as CD69 . In one previous study, CD69 was assumed to be considered as a new promising immunologic prognostic factor in B-CLL. Aim: In this study we aimed to detect CD69 expression in newly diagnosed patients with CLL by flow cytometry and correlate it with some other clinical and laboratory parameters in order to evaluate its role as a prognostic factor. Patients and Methods: CD69 expression was investigated by flow cytometry in 26 untreated newly diagnosed patients with chronic lymphocytic leukaemia ,and compared this with other standard prognostic parameters (β2- microglobulin , lymphocyte count , Rai stage and CD38 ). Results: The present study shows that about (34. 61% )of the CLL patient were positive for CD69 expression , while (65,38%) of patient were negative. CD69 expression is significantly associated with CD38 (P-0.002), Rai stages (p-0.0001) , lymphocyte count(p-0.0001) , β2-microglobulin (P-0.012). Conclusion: The results of this study demonstrated that CD69 is significantly associated with poor prognostic factors . This supports its introduction in a laboratory assessment of newly diagnosed patient with CLL and, possibly, in a prognostic scoring system for chronic lymphocytic leukemia.


Article
Association of GSTP1 Ile-105-Val Gene Polymorphism with Response to Treatment Among Iraqi Chronic Myeloid Leukaemia Patients
علاقة تعدد الاشكال الوراثية للمورث GSTPI Ile 105 Val مع الاستجابة للعلاج في مرضى سرطان الدم النقياني المزمن العراقيين

Authors: Atyab Abdul Hameed Alaqidi أطياب عبد الحميد العقيدي --- Maysem Mouayad Alwash ميسم مؤيد علوش
Journal: Al-Mustansiriyah Journal for Pharmaceutical Sciences مجلة المستنصرية للعلوم الصيدلانية ISSN: 18150993 Year: 2018 Volume: 18 Issue: 2 Pages: 133-141
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Pharmacogenomics is a relatively new study field that synergize pharmacology with genomics, analyzing the correlation between genetic variation and pharmacokinetics among patients. In the current study, we evaluated the potential effect of functional polymorphisms within gene encoding for Glutathione S transferase pi class (GSTPs) and treatment response among chronic myeloid leukaemia) CML) patients. GSTPs are multifunctional phase II biotransformation enzymes. Their main biologic role is to catalyze the conjugation to endogenous glutathione (GSH). In addition, they can alter drug potency in malignant cells. Polymorphic variants of these enzymes have been implicated in inter-patients' variability in drug response and outcome in CML patients. Aim of study: Evaluating the association between GSTP1 Ile-105-Val gene polymorphism and response to treatment among Iraqi CML patients. Method: A ‘PCR-RFLP’ assay was implemented to detect the polymorphic variants of codon 105 GSTP1 gene of forty Iraqi CML patients in chronic phase referring to the National Center of Haematology in the period between November 2017 and July 2018. Results: Our result revealed a statistically significant association between the GSTP1 genotypes and response to treatment. The variant genotypes were associated with inferior log reduction in BCR-ABL1 mRNA and poorer responses comparing to the wild genotype with P-value of 0.006, and 0.034 respectively.

ان تغاير الاشكال الوراثية للجينات المرتبطة بالتحولات الاحيائية للادوية قد تكون مسؤؤلة عن الاختلافات بين الافراد من حيث الاستجابة العلاجية اذ ان دراسة هذه التغييرات الجينية وتاثيراتها على الاستجابة الدوائية للمرضى قد تساعد في اختيار العلاج المناسب والتنبؤ بنتائج العلاج. في هذه الدراسة تم اجراء التنميط الوراثي للمورث (جلوتاثايون اس ترانسفريز بي 1) لاربعين مريضا بسرطان الدم النقياني المزمن العراقيين في الطور المزمن باستخدام تقنية "تغاير قطع التقييد لتفاعل البلمرة المتسلسل" ودراسة تاثيرات الانماط الجينية المختلفة على الاستجابة العلاجية للمرضى .اظهرت النتائج ان المرضى حاملي النمط الوراثي المتغاير كانو اكثر عرضة للاستجابة الضعيفة للعلاج من المرضى حاملي النمط القياسي. من هذا نستنتج ان الانماط الجينية المتغايرة للموروث (جلوتاثايون اس ترانسفريز بي 1) قد تحمل زيادة في خطر الاستجابة الضعيفة للعلاج لمرضى سرطان الدم النقياني المزمن, لذلك هنالك حاجة ملحة لأجراء المزيد من الدراسات لاجل تاكيد دور هذه التغايرات في حركية المواد الدوائية واستخدامها المحتمل كعوامل تنبؤ للاستجابة العلاجية في المستقبل


Article
Evaluation of the Intrinsic Pathway of Coagulation in a Sample of Iraqi Patients with Acute Myocardial Infarction

Author: *Maysem Mouayad Alwash F.I.B.M.S , *Alauldeen Mudhafar Zubair Alqasim F.I.B.M.S, **Salim R. Hammodi Ph.D.
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2016 Volume: 12 Issue: 1 Pages: 31-37
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Acute myocardial infarction (AMI) is one of the most common diagnoses in hospitalized patients. Increased plasma hemostatic markers were noted in acute myocardial infarction, indicating that the blood coagulation system is highly activated in those patients. Aims of the study: To study the level of intrinsic coagulation factors including (FVIII:C, FIX:C ,FXI:C ,FXII:C ) in patients with acute myocardial infarction. Type of the study: Cross –sectional study. Methods: Thirty patients (their age range is 48-68 years) were included in this study (9 female, 21 male) who were just admitted to the coronary care unit in AL-Yarmouk Teaching Hospital and diagnosed as having acute myocardial infarction patients, blood samples were taken from those patients . Twenty healthy subjects (6 female, 14 male) age and sex matched with the patients were included as a control group. The following investigations were done for both groups: 1.Packed cell volume(PCV%) .by microhaematocrit method. 2.FVIII:C assay. [ by parallel line bioassay of coagulationfactors]. 3.FIX:C assay. 4.FXI:C assay. 5.FXII:C assay. Results Mean FVIII:C (162.63%±17.22)was significantly (P value< 0.05)higher in patients with acute myocardial infarction than control group(94.70%±9.34).1-Mean FIX:C (151.20%±14.20) was significantly (P value <0.05) higher in acute myocardial infarction group than control group(94.10±8.51). 2-Mean FXI:C (146.30%±7.87) was significantly (P value <0.05) higher in acute myocardial infarction group than control group (102.00%±7.91). 3-Mean FXII:C(71.03±11.46) was significantly (P value <0.05) lower in acute myocardial infarction group than control group (119.00%±8.52). 4-There is inverse relationship between FXI:C and FXII:C in acute myocardial infarction group(P value -0.736). Conclusions Patients with acute myocardial infarction had significantly higher levels of FVIII:C,FIX:C,FXI:C than controls. FXII:C level was significantly lower in patients with acute myocardial infarction than control group. There is an inverse relationship between FXI:C and F XII:C in patients with acute myocardial infarction.

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