research centers


Search results: Found 3

Listing 1 - 3 of 3
Sort by

Article
The Significance of TP53 Gene Polymorphisms as A Risk Factor For Non-Hodgkin’s Lymphoma in Iraqi Patients

Authors: Maysoon Abdul-Ameer --- Ahmed Al-Salman
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 4 Pages: 778 -785
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Genetic factors including single nucleotide polymorphisms have been implicated as predisposing factors for large numbers of malignancies. When genetic disorders occur in a tumor suppressor gene, like TP53 gene, the results are expected to have devastating effects. The current study aimed to assess the effect of certain polymorphisms in TP53 genein the individual’s susceptibility to non- Hodgkin’s lymphomas among Iraqi patients. A total of 62 patients with these malignancies and 34 apparently healthy individuals were enrolled for this study. DNA was extracted from blood samples and fragment in TP53 corresponding for TP53 p.Arg72Pro, TP53 p.Pro47Ser and r.13494 G>A polymorphisms were amplified using specific primers. Genotyping was performed with restriction fragment length polymorphisms. The results revealed significant association of TP53 p.Arg72Pro polymorphism in both heterozygous and mutant homozygous genotypes with incidence of NHLs, while both TP53 p.Pro47Ser and r.13494 G>A polymorphisms had no such association. These results strongly indicate the importance of proline allele of TP53 p.Arg72Pro as a predisposing factor for NHLs.


Article
EFFECT OF TAMOXIFFEN ON HEPATIC CELL OF FEMALS ALBINE MICE ON THE ELECTRON MICROSOPICAL STUDY
دراسة تأثير عقار ثاموكسفين على خلايا الكبد في اناث الفأر الابيض على المستوى الخلوي

Author: Maysoon Abdul Ameer Ahmed Alsalman ميسون عبد الامير احمد السلمان
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2012 Volume: 53 Issue: 1 Pages: 68-72
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The effect of tamoxiffen which used in the breast cancer treatment were studies on liver tissue of 10 females white mice,Both groups (control and treated)were drinked with(0.4 mgkg) for 90 days. Clanges in liver tissue were seen through the examination of tissue and studied at the cellular level.The study aims to identify the damage caused by drug tamoxiffen on the liver tissue and its effects for long period

درس تأثير عقار ثاموكسفين المستخدم في علاج سرطان الثدي على نسيج الكبد في اناث الفئران البيضاء, حيث جرعت 10 أناث من الفئران البيضاء بالعقار المذكور بتركيز (0.4 ملغمكغم) لمدة 90 يوما قابلتها 10 أناث من الفئران البيضاء كمجموعة سيطرة , ولوحظ حدوث تغيرات نسيجية في الكبد من خلال فحص النسيج ودراسته على المستوى الخلوي. تهدف الدراسة الى التعرف على الاضرار التي تسببها عقار ثاموكسفين على نسيج الكبد وأثرها على تلف الكبد على الدمدى البعيد

Keywords


Article
Amplification of HER-2 Gene in Benign and Malignant Breast Lesions in a Sample of Iraqi Women
تضاعف جين في اورام الثدي الحميدة والخبيثة في عينة من النساء العراقيات

Loading...
Loading...
Abstract

Background: Breast cancer is the most common cancer worldwide. From several markers of this malignancy, HER-2 is considered to have a particular importance because it associates with the treatment and prognosis of the disease.Objective: To investigate the amplification of HER-2 gene in benign and malignant lesions of breast in a sample of Iraqi women.Patients and Methods: A total of 24 excisional breast biopsies were obtained from women with breast lesions. Biopsies were preserved in 10% formalin and undergone paraffin embedding according to the standard protocol. Four µm thick sections were prepared and placed on positively charged slide and stained with fluorescent in situ hybridization. The stained slides were examined with fluorescence microscope to detect HER-2 gene amplification.Results: Fourteen women were found to have benign lesions, while 10 were with malignant lesions. All benign lesions revealed two copies of the gene while seven of malignant cases showed positive results for HER-2 amplification (i.e more than 5 copies of the gene).Conclusion: These results support the idea that amplification of HER-2 could be considered as an indicator for tissue transformation into malignant lesion .

خلفية الدراسة: سرطان الثدي هو السرطان الاكثر شيوعا في جميع انحاء العالم. من بين عدة واسمات لهذا المرض يعد HER-2 ذو اهمية خاصة اذ انه يرتبط مع علاج ومآل المرض. اهداف الدراسة: هدفت هذه الدراسة الى استقصاء تضاعف في الاورام الحميدة والخبيثة في عينة من النساء العراقيات.المرضى والطرائق : تم الحصول على ما مجموعه 24 خزعة استئصالية من نساء يعانين من اورام الثدي. حفظت الخزعات بالفورمالين 10% وطمرت بالرافين وفقا للبروتوكول القياسي. تم اعداد مقاطع نسيجية بسمك 4 مايكروم ووضعت على شرائح مشحونة ايجابيا وصبغت بالتهجين الموضعي المتألق ثم فحصت تحت المجهر المتألق للكشف عن تضاعف جين HER-2.النتائج: اظهرت 14 امرأة اورام حميدة مقابل 10 نساء بأورام خبيثة ، وظهر الجين بنسختين في جميع الحالات الحميدة في حين اظهرت 7 حالات خبيثة نتائج ايجابية لتضاعف الجين ( اكثر من 5 نسخ من الجين).الاستنتاجات تدعم هذه النتائج فكرة ان تضاعف جين HER-2 يمكن ان يعد مؤشرا للتحول النسيجي باتجاه الورم الخبيث.

Listing 1 - 3 of 3
Sort by
Narrow your search

Resource type

article (3)


Language

Arabic and English (2)

English (1)


Year
From To Submit

2017 (1)

2016 (1)

2012 (1)