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Article
Sero-Epidemiological Study of Outbreak of Measles among Children in Diyala – 2009

Authors: Ismail I. Latif --- Mehdi SH. Al-Zuheiry --- Nadhim GH. Noaman
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 236-246
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Measles is a serious infectious disease in children. Despite reaching global measles vaccination coverage of 80% of individuals, measles virus (MV) remains the fifth leading cause of death and the most common cause of vaccine-preventable death in children under 5 years of age. Objectives: to determine the sero-epidemiological characters of the outbreak of measles among children in Diyala province in 2009.Subjects and methods: This study was done during the outbreak of measles in Diyala provinc ( spring and summer of 2009) in Al-Batool hospital of Pediatrics and Gynecology at Baquba city during a 2-month period from 1 April 2009 to 1 June 2009. A sample of 103 child patients presented with clinically suspected measles was studied by thorough history and physical examination with a determination of immunoglobulin M (IgM) antibodies in serum by enzyme- linked immunosorbent assay ( ELISA) testing for measles for each patient. Results: There was 58.3% (66 out of 103) positive blood samples for IgM of measles in children with clinically evident measles. The study showed that there was no significant difference in the distribution of children with measles positive by IgM according to their age and sex, according to residency, according to mothers′ previous vaccination status or previous infection with measles. On the other hand, the study revealed that the distribution of IgM positive measles was significantly more (p<0.05) in children who did not receive previous measles vaccine than those who received vaccination, and in children with low & medium economic status families than those with good status, respectively. Conclusion: It was concluded that the single serum assay of IgM antibodies by ELISA testing has medium sensitivity in the diagnosis of measles in children, there is an increasing susceptibility of infection with measles for infant less than one year of age and for children with poor family economic status.

Keywords

Measles --- IgM --- serology --- Children --- Diyala


Article
he Prevalence and the Quantification of Hepatitis C Virus among Thalassemia Patients using ELISA and PCR in Diyala Province
معدل الانتشار والتحديد الكمي لمرض التهاب الكبد الفايروسي نوع (ج) لدى المرضى المصابين بمرض فقر دم البحر الابيض المتوسط باستخدام تقنيتي ELISA و PCR في محافظة ديالى

Author: Mehdi SH. Al-Zuheiry ا.م.د. مهدي شمخي جبر الزهيري
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2016 Volume: 10 Issue: 2 Pages: 63-69
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Hepatitis C virus is the most common cause of post-transfusion hepatitis and end-stage liver disease in many countries. Regular blood transfusion in patients with hereditary hemolytic anemia, particularly thalassemia, has improved their overall survival, but carries a definite risk of acquisition of blood borne virus infections, especially viral hepatitis.Objective: To determine the prevalence and the quantification of HCV infection among thalassemia patients in Diyala province by using enzyme- linked immunosorbent assay and polymerase chain reaction techniques.Patients and Methods: This study was conducted on 215 thalassemia patients in the thalassemia unit in Al-Batool Teaching Hospital of Maternity and Pediatrics in Baqubah city- Diyala province during the period from September 2013 to December 2015. Demographic data were obtained from the patients records. Sera were tested with anti-HCV assays by use enzyme linked immunosornbant assay and positive result were tested by a Real time PCR technique to assess HCV infection.Results: A total of 215 thalassemic patients, 26 (12.1%) patients were HCV positive by enzyme linked immunosornbant assay and only 3 of these 26 patients were positive by RT-PCR. The rate of HCV infection in thalassemic patients is associated directly with the number of blood transfusions and the age of the patients and It is more in the period before 1996.Conclusion: The prevalence of HCV infection in thalassemic patients is relatively low in Diyala province in comparison with other areas and PCR technique is essential to detect and treat active infection.

خلفية الدراسة: ان مرض التهاب الكبد الفايروسي نوع (ج) هو السبب الاكثر لالتهابات الكبد الناتجة عن عمليات نقل الدم وامراض الكبد ذات المرحلة النهائية في العديد من الدول. ان نقل الدم المنتظم عند مرضى فقر الدم التحللي وخاصة مرضى فقر دم البحر البيض المتوسط قد ساعد في تحسين مستوى العيش العام , لكنه يحمل خطرا اكيدا يتمثل في انتقال الفايروسات التي تنتقل خلال الدم وخاصة مرض التهاب الكبد الفايروسي.اهداف الدراسة: لتحديد معدل الانتشار والتحديد الكمي لمرض التهاب الكبد الفايروسي نوع (ج) لدى مرضى فقر دم البحر الابيض المتوسط في محافظة ديالى باستخدام تقنيات ELISA و PCR.المرضى وطرائق العمل: اجريت هذه الدراسة على 215 مريض مصاب بمرض فقر دم البحر البيض المتوسط من المرضى المسجلين في وحدة فقر دم البحر البيض المتوسط في مستشفى البتول التعليمي للنسائية والاطفال للفترة من ايلول 2013 الى كانون الاول 2015. تم تسجيل المعلومات من سجلات المرضى وتم فحص عينات امصال الدم من المرضى لمضادات مرض التهاب الكبد الفايروسي نوع (ج) باستخدام تقنية ELISA و تم فحص النماذج الموجبة بتقنية ELISA بفحص PCR لتقييم مرض التهاب الكبد الفايروسي نوع (ج).النتائج: من مجموع 215 مريض بمرض فقر دم البحر الابيض المتوسط وجد هنالك 26 مريضا (12,1%) مصابا بالتهاب الكبد الفايروسي نوع (ج) باستخدام تقنية ELISA وفقط 3 مرضى من هؤلاء ال 26 مريضا كانوا ذوي نتائج موجبة باستخدام تقنية PCR. ووجد ان معدل الاصابة بمرض التهاب الكبد الفايروسي نوع (ج) لدى مرضى فقر دم البحر الابيض المتوسط يتناسب طرديا مع عدد مرات نقل الدم وعمر المريض ويكون هذا المعدل اكبر قبل العام 1996.الاستنتاجات: ان معدل انتشار مرض التهاب الكبد الفايروسي نو ع (ج) لدى مرضى فقر دم البحر الابيض المتوسط هو معدل واطئ نسبيا بالمقارنة مع المناطق الاخرى, وان تقنية PCR تكون حيوية لتحديد ومعالجة هذا المرض.


Article
The Prevalence of Rotavirus Infection in Baquba- Diyala Province

Authors: Mehdi SH. AL-Zuheiry --- Abbas A. Al-Duliami --- Abdul-Razak SH. Hasan --- Abdul-Kadir Y. Al-Azawi
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 16-27
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Rotavirus infection is the leading single cause of severe diarrhea among infants and young children. More than 500,000 children under 5 years of age die from rotavirus infection each year, and almost 2 million more become severely ill.Objectives: To determine the prevalence of rotavirus infection among patients with diarrhea in Baquba- Diyala province, and to explore the effect of certain relevant factors.Materials and methods: The present study was extended from 1/July/2007 to 1/September/ 2008 in Baquba city. A total of 300 fecal specimens were collected from patients suffering from acute diarrhea. The patients include 136 (45.3%) females with mean age (7.8 ± 4.7) years, and 164 males with mean age (5.3± 3.4) years. BioRad-Rota kit is a highly sensitive agglutination test was used for detection of rotavirus in fecal specimens. Bacterial co-infections were identified by culturing on differential and selective media, and the final diagnosis followed the standard bacteriological criteria. Parasitic co-infections were detected by general stool examination. All data were statistically analyzed.Results: The results showed that the overall infection rate by rotavirus among patients was 20.3%, and the highest infection rate was among those below 5 years of age. Rotavirus infection among adult patients was also recorded. Females had an insignificantly higher infection rate compared to males (22.1% vs. 18.9%). Patients consuming river's water had significantly higher infection rate compared to those used municipal or tank water (34.5%, 14.1% and 18.5%) respectively. Patients (below 2 years) feed artificially had higher infection rate com College of Vet. Med. Diyala University pared to those on breast or mixed feeding (28.2%, 19.1% and 18.8%) respectively. Although, the rotavirus infections were recorded around the year, the highest infection rate was during spring and winter seasons (22.5% vs 21.4%). It was also found that neither bacterial nor parasitic co-infections were significantly associated with rotavirus infection.Conclusion: Rotavirus infection as a cause of acute diarrhea was common in Diyala province, and particularly affects children below 5 years of age.

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