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Article
Study of Some Purine Metabolic Enzymes in Sera of Patients with Renal Failure

Authors: Moaed Emran Al.Gazally --- Sahib Ali Mahdi --- Sawsan Hassan Kadhim
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2010 Volume: 7 Issue: 1-2 Pages: 286-290
Publisher: Babylon University جامعة بابل

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Abstract

one hundred seven patients ( 51 male and 56 females ) suffering from renal failure admitted to Mirjan Teaching Hospital were included . Twenty healthy individuals were included as control group. Blood samples were collected and the sera were separated . It was found that renal failure was more predominant among the patients age group rang from 40 – 70 years old. Besides , it was found out that the mean values of some biochemical parameters importance for the detection of the disease which were investigated in this study where higher than the normal . It was found that urea and creatinine mean value for all patients were highly significant if compared to control group. The mean value (M ± SD) of Adenosine deaminase activity was decreased significantly (14.30 ± 10.47) in all patients with renal failure if compared to the control group (63.80 ± 22.98). On the other hand ,the mean of xanthine oxidase activities also reduced but not significantly (4453.98 ± 694.86) if compared with control group ( 4669.45 ± 811.80 )

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Article
The Role of Thyrotropin Hormone Receptor Antibody (TRAb) in Distinguishing between Autoimmune and non-Autoimmune Disease

Authors: Saif Ali Muttaleb --- Muhanned Abbas Al-shalah --- Moaed Emran Al-Gazally
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2013 Volume: 10 Issue: 1 Pages: 153-161
Publisher: Babylon University جامعة بابل

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Abstract

The present study was intended to investigate Thyrotropin Receptor Antibody (TRAb) level in hyperthyroidism. A total of 65 patients and 25 apparently healthy as a controls were assessed. Twenty-seven of the 65 patients had Graves' disease, twenty-one had toxic nodular goitre and the remaining group had toxic nodule. In comparison with the control group, patients with diffuse goitre showed a highly significant increase in TRAb (P<0.01) significant increase in TRAb (p < 0.05) in nodular toxic goitre, while non-significant (p> 0.05) found in nodule toxic. Thyroid function tests appear significant increase (P<0.01) T4, T3 while significant decrease in thyroid stimulating hormone TSH concentrations. The incidence of positive TRAb assay in diffuse goitre group was 89%, whereas 24% were positive in toxic nodular goitre group and 6% in toxic nodule. It can be concluded that as a practical method, TRAb assay may be useful in making a differential diagnosis of Graves' disease rather than other autoimmune thyroid diseases (toxic nodular and toxic nodule).

تضمنت الدراسة معرفة مستوى الأضداد الذاتية للمستقبلات للهرمون المحفز للغدة الدرقية .حيث شملت الدراسة 65مريضا مصابا بفرط إفراز هرمونات الغدة الدرقية و 25 شخص ظاهريا أصحاء كمجموعة سيطرة .27 من المرضى لديهم المرض المناعي التلقائي , و 21من المرضى لديهم غدة درقيةُ متعددة العقد السامّةُ والمجموعةُ الباقيةُ لديها عقدةُ سامّةُ. بالمقارنة مع مجموعة السيطرة, أظهرت نتائج المرضى المصابين بالمرض المناعي التلقائي المنتشر (Graves' disease ) وجود زيادة عالية المعنوية في تركيز المضاد الذاتي للمستقبل هرمون الغدة الدرقية ((P<0.01 كذلك زيادة معنوية في التركيز للمرضى المصابين بمتعدد العقد السامة P<0.05) ) ,بينما لا يوجد فرق معنوي للمرضى المصابين بالعقدة السامة. اختبار هرمونات الغدة الدرقية يظهر زيادة عالية المعنوية في تركيز كل من هرمون T3 و T4 ((P<0.01 بينما TSH اظهر نقصان عالية المعنوية بالتركيز((P<0.01. وجد أن نسبة 89 % من الأضداد الذاتية للمستقبلات هرمونات الغدة الدرقية موجودة في مجموعة المرض المناعي التلقائي ونسبة 24 % لمجموعة المرضى متعدد العقد السامة ونسبة 6 % لمجموعة مرضى العقدة السامة. نستنتج من ذلك يمكن استخدامه كطريقة عملية في التشخيص بين المرض المناعي التلقائي (Graves' disease ) عن الأمراض الغير المناعيه الأخرى المتمثلة بالعقيدات السامة والسامة متعددة العقيدات.


Article
Study of the changes of vascular endothelial growth factor (VEGF),antioxidant capacity and oxidative stress in the sera of Iraqi patients with ischemic stroke

Authors: Moaed Emran Al-Gazally --- Ali Hmod Al-Saadi --- Ahmed Faisal Obed
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2014 Volume: 22 Issue: 9 Pages: 2376-2371
Publisher: Babylon University جامعة بابل

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Abstract

Background: Ischemic stroke is death of brain tissue due to interruption of blood flow to a region of the brain, caused by occlusion of a carotid or vertebral artery or , less likely, a cerebral vein. It accounts for 85% of all stroke cases. Ischemic stroke is rapidly developing clinical signs of focal ("or global ) disturbance of cerebral function, with symptoms lasting 24 hours or longer, or leading to death.Aim of the study: To find out the relation between vascular endothelial growth factor , antioxidant capacity and oxidative stress in the patients with acute ischemic stroke cases. Patients and Methods: Our study was conducted on (60) patients with acute ischemic stroke and (30) apparently healthy subjects were taken as control group. The sera obtained from the blood of patients and healthy subjects were used to measure the concentrations of vascular endothelial growth factor (VEGF), total antioxidant capacity (TAC) and malondialdehyde (MDA). Serum VEGF concentration was determined by using enzyme- linked immunosorbent assay (ELISA) . Total antioxidant capacity and malondialdehyde (MDA) were determined by using colorimetric method.Results: In comparison with the control group, the patients with acute ischemic stroke show a significant increase in VEGF (P < 0.01) and MDA (P < 0.01) concentrations, and a significant decrease in TAC concentration (P < 0.01) . Also we found a significant elevation of VEGF (P < 0.05) in hypertensive patients with ischemic stroke compared to normotensive patients .On the other hand , there was a significant decrease (P < 0.05) of TAC concentration in smoker patients compared to non smoker ones against a significant increase (P < 0.05) of MDA concentration of smoker patients compared to non smoker patients. Also MDA level found to be increase significantly ( P < 0.05) in ischemic stroke patient who were also diabetic in compared to non diabetic patients . Significant positive correlation also observed between VEGF and MDA concentrations (r=0.374,P<0.05) in patient group .Conclusion: Our results suggest a functional interplay among oxidative stress, antioxidants, and VEGF in patients with ischemic stroke.

المقدمة : السكتة الدماغية الاقفارية هي موت جزء من نسيج الدماغ بسبب انقطاع تدفق الدم إلى ذلك الجزء ، بسبب انسداد الشريان السباتي أو الفقري أو في حالات نادرة انسداد الوريد الدماغي. تمثل السكتة الدماغية الاقفارية 85 % من كل حالات السكتة الدماغية . تتطور الأعراض السريرية لمرضى السكتة الدماغية الاقفارية بسرعة مسببة اضطرابا جزئيا أو كليا في وظيفة الدماغ ، تستمر هذه الأعراض 24 ساعة أو أكثر أو قد تؤدي إلى الموت .الهدف من البحث: لمعرفة العلاقة بين عامل النمو البطاني الوعائي والقدرة المضادة للأكسدة وجهد التأكسد في المرضى المصابين بالسكتة الدماغية الاقفارية .المرضى وطرق العمل: تضمنت الدراسة (60) مريضا مصابا بالسكتة الدماغية الاقفارية و(30) شخصا معافى ظاهريا لمجموعة سيطرة . تم استخدام الأمصال التي تم الحصول عليها من دم المرضى والأصحاء لقياس تراكيز عامل النمو البطاني الوعائي (VEGF)، و القدرة الكلية المضادة للأكسدة (TAC) والمالونديالدهيد (MDA). تم تحديد تركيز VEGF في الدم باستخدام المقايسة المناعية المرتبطة بالإنزيم (ELISA). وتم تحديد إجمالي القدرة المضادة للأكسدة والمالونديالدهيد (MDA) باستخدام الطريقة اللونية.النتائج: بالمقارنة مع مجموعة السيطرة فان مرضى السكتة الدماغية الاقفارية اظهروا زيادة معنوية في عامل النمو البطاني الوعائي وتركيز المالونديالدهيد (P < 0.01) وانخفاضا معنويا في الجهد الكلي المضاد للاكسدة (P < 0.01) . يوجد ايضا ارتفاع معنوي في عامل النمو البطاني الوعائي في مرضى السكتة الدماغية المصابين بارتفاع ضغط الدم مقارنة مع المرضى . من ناحية أخرى، كان هناك انخفاض معنوي (P < 0.05) في تركيز القدرة المضادة للأكسدة في المرضى المدخنين مقارنة مع تلك في المرضى غير المدخنين ، مقابل زيادة معنوية (P < 0.05) في تركيز المالونديالدهيد في المرضى المدخنين مقارنة مع المرضى غير المدخنين. كما وجدت زيادة معنوية في تركيز المالونديالدهيد (P < 0.05) في مرضى السكتة الدماغية المصابين بالسكري مقارنة مع المرضى غير المصابين بالسكري.كذلك لوحظ وجود علاقة ايجابية معنوية بين عامل النمو البطاني الوعائي وتركيز المالونديالدهيد (r = 0.374 , P < 0.05) في مجموعة المرضى .الاستنتاج : تشير نتائج الدراسة الحالية إلى وجود تفاعل وظيفي بين الاكسدة، ومضادات الأكسدة، وعامل النمو البطاني الوعائي في المرضى الذين يعانون من السكتة الدماغية الاقفارية.


Article
Evaluation The Serum Total Protein in Patients with Diabetes Mellitus (Type I and Type II) and Study Genetic Level of Glutathione-S-Transferaseµ 1

Authors: Nisreen Jalal Mohammed --- Moaed Emran AL-Gazally --- Maysam Ali Ameen Awadh
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 625-631
Publisher: Babylon University جامعة بابل

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Abstract

Diabetes mellitus is a group of metabolic diseasesdue to defect in insulin secretion or action or both.Hyperglycemia in diabetes creates free radicals .These free radicals produce oxidative stress and thus debilitate the endogenous antioxidant defense system.If the amount of insulin available is insufficient, cells response badly to the effects of insulin or if the insulin itself is abnormal. The net effect is persistently elevating levels of blood glucose, low protein synthesis.The current study evaluate the biochemical changes in diabetes mellitus patients in using different medications and investigate the glutathione S-transeferase M1 gene deletion in different treatments. The present study was conducted on (75) diabetic patients, (25) of them were treated with insulin, (25) were using insulin and metformin and the last (25) were on metformin and glibinclimide .The study also included (25) apparently healthy subjects were taken as control group. The blood sampleswere collected from Merjan Teaching Hospital from November 2013 to April 2014.The total serum protein was measured by colorimetric method described by Gornall.LSD test showed that there was significant difference between insulin & metformin group and metformin &glibinclimide in total serum protein (p ≤0.05). Metformin &glibinclimide group was less mean difference with the control group. In the gene level of the study, genotyping of glutathione S-transferase mu 1gene by PCR were defined as GSTM1 and GSTM0 or deletion association to the present and absences of the guanine nucleotide in the gene sequence. There was statistically significant difference in the genotyping distribution and the frequency of GSTM0 among study groups were 44% for insulin and metformin group, 68% for metformin and glibinclimide, 44% insulin and 28% for control healthy group.there was significant decrease in serum total protein in diabetic patients. Genetic polymorphism of glutathione S-transferase mu 1gene may be considered as risk factor for both types of diabetes mellitus.


Article
Assessment of Antioxidant Status and Ceruloplasmin in Early and Advanced Stages of Sporadic Colorectal Cancer Patients whom Receiving Adjuvant Chemotherapy

Authors: Moaed Emran Al-Gazally --- Alaa Sadiq Al-Awad --- Hamza Hashim Kzar
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 780-791
Publisher: Babylon University جامعة بابل

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Abstract

One of most frequent malignant disease in the developed countries is colorectal cancer and it is the seventh most common cancers among population of Babylon province/Iraq. It was reported that oxidative stress status is being play a very important roles in development of carcinogenesis. The purpose from this study was assessment of antioxidant status in early and advanced stages of patients with sporadic colorectal cancer whom received adjuvant chemotherapy. This investigate was done by measuring of total antioxidant capacity(TAO-C), Ceruloplasmin and its related trace elements ,copper and Zink. All patients included in this study were receiving adjuvant chemotherapy regimen and subdivided into two groups according to Duke´s classification of malignant into early stages (A+B) and(C+D) advanced stages, cancer site (colon and rectum), and according to the number of dosage of chemotherapy regimen(half dosage and total dosage). ELISA methods were used to assess serum Cp concentration and colorimetric method for measurement of serum TAO-C ,while concentration of Cu and Zn were determined by atomic absorption spectrophotometer ( AAS). Cu/Zn and Cu/Cp ratio were calculated mathematically. The results were showed highly significant decreasing(p<0.05) in T-AOC and Zinc concentration in stages (C+D) subgroup compared to (A+B) and control group. Both Ceruloplasmin and copper were found markedly increased in stages (C+D) compared to (A+B) stages and control group. Cu/Zn ratio was increased significantly in advanced stages and controls while Cu/Cp ratio was markedly decreased in advanced stages compared to early stages and controls. In conclusion the results of present study suggests that TAO-C and/or Cp and its related elements are monitoring factors and prognostic indicators could be valuable during the treatment with adjuvant regimen for sporadic colorectal cancer patients.

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