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Article
THE EFFECT OF DIFFERENT CULTURE MEDIA ON IN VITRO FERTILIZATION OF MICE

Authors: Mohammed A. Ibrahim --- Hazim I. Al-Ahmad --- Wasnaa H. Mohammed
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2010 Volume: 13 Issue: 4 Pages: 147-150
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The effect of different types of media on in vitro fertilization (IVF) of mice oocyte wasinvestigated. IVF was carried out in RPMI-1640 medium at recommended temperature (37 C) andpH (7.4). The obtained results showed that 63.73% of used oocytes were fertilized, and thepercentage of abnormal embryos was 27.58%. While, using other culture media at sametemperature and pH, gave low percentage of fertilization and higher yield of abnormal embryos.Thus, using Medicult IVF, Earl s and TCM-199 were gave fertilization percentages equivalent to38.75, 42.42 and 61.45% respectively; whereas, the percentages of abnormal embryos were 32, 35.7and 33.89 % respectively.The reported results indicate that composition of various types of culture media have significanteffect on the rate of fertilization and abnormal embryos.


Article
Evaluationof Curing Depth of Bulk-Fill Resin Composite (A comparative study)

Authors: Salam A. Al-Araji --- Mohammed A. Ibrahim --- Hala A. Ragab
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 1 Pages: 310-316
Publisher: Babylon University جامعة بابل

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Abstract

This study evaluated the depth of cure (DOC) of recently introduced resin composites for posterior use, bulk filled flowable composite (SureFil® SDR® flow DENTSPLY Caulk) at different depth. 30 specimens were prepared and divided into two Groups,Group 1: specimens with 2 mm depth, Group 2: specimens with 4mm depth. The composite specimens were prepared by using molds of different depth, one of them with was a hole of 4 mm depth and 4 mm internal diameter and the other was with a depth of 2 mm at the same diameter. The hole was bulk filled with SDR flowable composite resin and light cured for 20 seconds with a modern high-intensity LED curing unit (Elipar™ S10, 3M.US.), followed by 24 hours storage in complete darkness incubator at 37 C◦. The degree of conversion was measured on the top and the bottom for both depths using Fourier transform infrared (FTIR) spectroscopy. A bulk filled flowable (SDR)revealed a significant difference in degree of conversion (DOC) when the bottom surface of the specimen witha 2mm depth (DOC=76.27112163) wascompared with the bottom surface of the 4mm depth specimen (DOC=73.92160935). The 2mm depth wasshown statistically significantly higher mean values degree of conversion than 4 mm depth (P-value = 0.032). There was no statistically significant difference in the degree of conversion mean values between the top and the bottom surfaces of the specimen of the same group (P >0.05).Upon the result of thisin vitro study, it was found that the degree of conversion of the SDR bulk-fill flowable resin compositewasaffected by depths. As the depth of the resin compositewas increased the degree of conversion was decreased.


Article
Comparative Study Between Therapeutic Effects of Melatonin and Zinc in Lead induced Toxicity in Rats

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Abstract

The present study was designed to investigate the therapeutic effects of melatonin as well as zinc in combination or each one alone against the hepatic and renal toxicity of lead in male rats. Five groups of animals were allocated as follows: Group I, includes 12 rats injected subcutaneously with 0.2 ml physiological saline for 60 days; groups II and III, each includes 12 rats, injected with lead acetate 100 mg/kg/day s.c for 30 days, followed by treatment with intraperotoneal injection of physiological saline (0.2 ml) or melatonin 30mg/kg/day for the next 30 days. Group IV, include 12 rats, injected with lead acetate 100 mg/kg/day s.c for 30 days, followed by treatment with intraperotoneal injection of zinc sulphate 1.5 mg/kg/day for the next 30 days. Group V, includes 12 rats injected with 100mg/kg lead acetate s.c for 30 days followed by treatment with intraperitoneal injection of 30 mg/kg/day melatonin and 1.5mg/kg/day zinc sulphate for the latter 30 days. At the end of treatment period, the rats were sacrificed by an overdose (100mg/kg) of thiopental (twenty-four hour after the last injection). Laparotomies were performed to obtain the livers and kidneys for the assessment of tissue damage. Oxidative stress parameter (MDA), hemoglobin content, liver and renal functions, and histological appearance of the studied organs were evaluated and compared with that of negative and positive controls. Treatment with melatonin or zinc reverses the damage induced by lead in many organs and tissues through the reduction of MDA levels in RBCs, liver and kidneys; in addition to the improvement in the indices of the functions of the organs studied. These findings demonstrated that addition of zinc to melatonin are capable of further reversing damage of rat tissues caused by successive doses of lead acetate, and animals had restored their organ functions due to combined treatment.

صممت هذه الدراسة لتحديد التأثيرات العلاجية للميلاتونين وكذلك الزنك, أما كل على حدا أو بصورة مجتمعة على التأثيرات السمية بواسطة الرصاص على الكبد والكلية عند الجرذان الذكور.تم تعريض مجاميع الحيوانات الخمسة التي تمت دراستها إلى جرعة يومية مقدارها 100ملغم/كغم من مادة خلات الرصاص تحت الجلد لمدة شهر واحد. تم استخدام (30ملغم /كغم) من مادة الميلاتونين او الزنك عن طريق البريتون بعد استمرار التسمم للرصاص لمدة شهر آخر. تمت متابعة التغيرات الحاصلة في معايير فرط الأكسدة ، محتوى الدم من الهيموغلوبين ،فعالية الكبد والكليتين إضافة إلى التغييرات النسيجية التي طرأت على الأعضاء التي تمت دراستها مقارنة مع المجموعة القياسية (السلبية والايجابية). يمكن الأستنتاج من هذه النتائج أن الميلاتونين والزنك لهم القابلية على منع الضرر النسيجي نتيجة للتعرض الى جرع متعاقبة من خلات الرصاص وأن الحيوانات المختبرية لها القدرة على أستعادة فعالية أعضاءها الحيوية نتيجة للعلاج بالميلاتونين والزنك معا بعد التعرض للرصاص.

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Article
Eggshell Powder as An Adsorbent for Removal of Cu (II) and Cd (II) from Aqueous Solution: Equilibrium, Kinetic and Thermodynamic Studies

Authors: Ziad Tark Abd Ali زياد طارق عبد علي --- Mohammed A. Ibrahim محمد عبدالخالق ابراهيم --- Huda M. Madhloom هدى مهدي مظلوم
Journal: AL-NAHRAIN JOURNAL FOR ENGINEERING SCIENCES مجلة النهرين للعلوم الهندسية ISSN: 25219154 / eISSN 25219162 Year: 2016 Volume: 19 Issue: 2 Pages: 186-193
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The adsorption of Cu (II) and Cd (II) ions onto eggshell was investigated. The effects of contact time, initial pH of solution, adsorbent dosage, initial metal concentration, agitation speed, and temperature were studied in batch experiments. The maximum adsorption capacities for Cu(II) and Cd(II) were 8.4 and 7.01 mg/g, respectively. Thermodynamic parameters such as Gibbs free energy change, Enthalpy change and Entropy change have been calculated. These thermodynamic parameters indicated that the adsorption process was thermodynamically spontaneous under natural conditions and the adsorption was endothermic in nature. Experimental data were also tested in terms of adsorption kinetics, the results illustrated that the adsorption process was following pseudo second- order kinetics. In addition Fourier transfer infrared spectroscopy (FTIR) analysis suggests that Cu (II) and Cd (II) interact with metal oxides and -OH functional group present in eggshell powder.

تم التحقق في هذا البحث من استخدام قشور البيض كمادة ممتزة لامتزاز ايونات النحاس والكادميوم. وتم دراسة تاثير عدة عوامل منها زمن التوازن, الدالة الحامضيه الابتدائيه للمحلول, كمية الماده الممتزه, التركيز الابتدائى, سرعة الرج, بالاضافه الى درجه الحراره. وكان الحد الاعلى لسعة الامتزاز (8.4mg/g) و (7.01mg/g)لايونات النحاس والكادميوم على التوالي. كذلك تم حساب بعض العوامل الثرموديناميكية مثل Gibbs free energy Entropy, Enthalpy وقد اشارت هذه العوامل بان عملية الامتزاز هي عمليه ثرموديناميكيا تلقائية وماصه للحرارة . النتائج العمليه اشارت ايضا بان عمليه الادزوربشن تتبع ل" pseudo second- order kinetics " . واخيرا تم اجراء فحص FTIR الذي اشار بوجود تداخل بين ايونات النحاس والكادميوم مع اكاسيد المعادن والمجموعه الفعالة (-OH) الموجوده في قشور البيض.


Article
Medical students’ attitudes concerning medical ethics courses in AL-Kindy medical college 2013-2014

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Abstract

ABSTRACTBackground: In Medical ethics education, improving medical student’s attitudes toward respecting the right of patients is an essential task. The medical students’ attitude has been affected by social, educational and personality background factors.Objective: To investigate medical student’s attitudes regarding medical ethics courses.Method: The study was conducted in Al-Kindy College of Medicine on academic year (2013 -2014) for the period from January to September. A cross- sectional study design was adopted with a self- administered questionnaire form distributed to medical students in the 5th-6th under graduate grades. The questionnaire consisted of 31 items relevant to student’s opinion about attitudes concerning ethics courses.Result: Out of overall 113 students 90(78.1%) responded, the majority 88.9% considered medical ethics teaching for medical students an important issue, and 85.5% had general interest in learning more about medical ethics. However, about 84.4% agreed that medical ethics teaching would influence the attitudes and behaviors of doctors, and improvepatient doctor relationship, but about 65.6% of students were confident with their ability to recognize significant ethical problems in clinical practice. Only 46.7% knew how to proceed when a patient is mentally incompetent.Conclusion: Medical students have positive knowledge andattitude about the medical ethics teaching, but they may notbe adequately prepared to meet the challenges of clinicalpractice. There should be incorporation of creative andintegrated ethics curriculum to start in the first medical grade,with emphasis on ethical aspect of daily medical practice.

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