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EXPRESSION OF Fas AND FasL IN TROPHOBLASTIC TISSUE OF WOMEN WITH SPONTANEOUS AND INDUCED ABORTION USING INSITU HYBRIDIZATION TECHNIQUE

Authors: Abdul-Razzak H. Ahmed عبد الرزاق حردان احمد --- Mohammed R. Ali محمد رزاق علي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 2 Pages: 147-151
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundApoptosis of trophoblastic cells may play a role in the pathogenesis of abortion, and one mechanism of apoptosis is Fas receptor and legand system.ObjectiveEstimate the level of Fas and FasL in trophoblastic tissue of aborted women.MethodsIn this study, 25 women with spontaneous abortion and 5 women with induced abortion were included from attendants of Gynecology department at Al-Kadhimiya Teaching Hospital in Baghdad. In-situ hybridization (ISH) tests were done to detect the level of expression of Fas and FasL in trophoblastic tissue.ResultsThe highest percentage of expression of Fas and FasL was found in the trophoblastic tissue of females with spontaneous abortion (34.36% for Fas and 31.86% for FasL), while the expression of them was low in induced abortion group (9.2% and 9.4%, respectively).ConclusionThe data strengthen the possibility that the apoptosis of trophoblastic cells via enhanced expression of Fas-FasL system is an important mechanism of spontaneous abortion.Key wordsMiscarriage, Trophblast, Apoptosis, Fas, FasL, ISH.

Keywords

Miscarriage --- Trophblast --- Apoptosis --- Fas --- FasL --- ISH


Article
8.EXTRACTION, PURIFICATION AND THERAPEUTIC USE OF BACTERIOPHAGE ENDOLYSIN AGAINST MULTI-DRUG RESISTANT PSEUDOMONAS AERUGINOSA: AN IN-VITRO AND IN-VIVO STUDY

Authors: Mohammed R. Ali محمد رزاق علي --- Shurooq R. Kadhim شروق ريس كاظم --- Ahmed S. Abdulamir احمد صاحب عبد الامير
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 4 Pages: 405-412
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Persistent infection with multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) represents a real problem for health care providers. Bacteriophage lytic enzymes or lysins are highly evolved molecules that have been specifically developed by phages to quickly and efficiently allow their progeny to be released from the host bacterium while destructing that bacterium.Objective: Isolation of endolysin from P. aeruginosa bacteriophages, and administering them systematically in vivo lab animal and measuring their therapeutic efficacy as well as evaluation of their biosafety.Methods: This study was performed from March 2015 – August 2017, during which 50 bacteriological samples of P. aeruginosa were collected, and examined for their antibiogram, then bacteriophage cocktails were done for 5 resistant strains of them. Endolysins were extracted from their corresponding bacteriophages and characterized. The enzymatic and antibacterial activities as well in vivo therapeutic efficiency of these enzymes were investigated.Results: This study showed that the extracted endolysin from these bacteriophages was effective in treating laboratory mice from bacteremia with P. aeruginosa and saving their lives when injected intraperitoneal.Conclusion: Endolysin can be extracted directly from their bacteriophages and used effectively in proper doses to treat bacteremia in mice.Keywords: Pseudomonas aeruginosa, bacteriophage, endolysin, MDRCitation: Ali MR, Kadhim SR, Abdulamir AS. Extraction, purification and therapeutic use of bacteriophage endolysin against multi-drug resistant Pseudomonas aeruginosa: An in-vitro and in-vivo study. Iraqi JMS. 2018; 16(4): 405-412. doi: 10.22578/IJMS.16.4.8

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