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Article
In vitro effects of hypertonic saline solution on whole blood clotting

Author: Mohammed Shnain Ali;
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2013 Volume: 6 Issue: 1 Pages: 1568-1572
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: hypertonic saline solution is frequently used to in the management ofhemorrhagic shock.Objective: To determine the effects of hypertonic (7%) saline solution on wholeblood coagulation (clotting) in vitro.Patients and methods: Ten healthy volunteers (5 males and 5 females with age range of 28-44 years) with no previous medical history of bleeding tendency and no medical history ofdrug intake were studied after their consent. Hypertonic (7%) saline was preparedimmediately before use. For each patient, 4 ml of venous blood sample was obtained andinvestigated as follows:Undiluted 450 µl of whole blood for baseline coagulation tests [prothrmbin Time (PT),activated partial thromboplastin time (APTT) and thrombin time (TT)]. After that, the sameabove tests were done after dilutions of whole blood (i.e whole blood replacement byhypertonic saline and isotonic saline for each dilution). An Isotonic (0.9%) saline dilutionserves as control. Blood volume replacements at 2.5%, 5%, 7.5% and 10% were done andstudied.Results: The baseline coagulation tests (PT, APTT, and TT) were normal for all 10 (100%)of subjects. At 2.5% and 5% of blood volume replacement PT, APTT and TT were slightlyprolonged, but showed no statistically significant difference between hypertonic (7%) salineand the control (isotonic saline) while PT, APTT and TT were prolonged in hypertonic (7%)saline with statistically significant difference (p<0.05) from isotonic (0.9%) saline at 7.5%and 10% of whole blood volume replacement in 10, (100%) of subjects.Conclusion: The study showed that hypertonic (7%) saline disturbs coagulation in vitro whenit replaces 7.5% or 10% of whole blood volume.


Article
Prevalence of Anti-Rh (D) Antibody in Karbala

Author: Mohammed Shnain Ali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2013 Volume: 6 Issue: 2 Pages: 1644-1647
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Neither Rh(D) positive individuals nor Rh (D) negative individualsnormally have anti-Rh(D) antibody in their blood; however, Rh (D) negativeindividuals have the ability to form such antibody when they are transfused withRh(D) positive blood.Objective: To determine the prevalence of anti-Rh (D) antibody in Karbala.Patients and methods: From January 2012 to March 2013, 226 patients (127 males and 99females with age range of 20-49 years) were studied after their consent. For each patient, 4ml of venous blood sample was obtained and investigated as follows:Two ml of blood were transferred to EDTA tube for ABO and Rh(D) grouping using thecommercially available kit (monoclonal anti-A, anti-B and anti-D) by doing the ordinary tilemethod (equal volumes of blood and reagent were mixed on tile looking visually for theagglutination for positive reaction and positive result. Absence of agglutination meansnegative reaction and negative result). The other 2 ml of blood were transferred to plain tube,centrifuged and serum is investigated for anti-Rh(D) antibody in Rh(D) negative individualsby double dilutions of each serum and antibody titration method.Results: Out of 226 individuals, 186 (82.3%) were Rh(D) positive, 40 (17.7%) were Rh(D)negative, and 2 were found to be positive for anti-Rh(D) antibody. The prevalence of anti-Rh(D) antibody is 0.88%.Conclusion: The study showed that the prevalence of anti-Rh(D) antibody in Karbala is0.88%.


Article
Comparison between digital hematocrit reader and standard centrifugation based hematocrit measuring instrument.
مقارنة بين قراءة نسبة الدم بالجهاز الرقمي والجهاز القياسي المعتمد على الطرد المركزي.

Author: Mohammed Shnain Ali
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2014 Volume: 12 Issue: 2 Pages: 12-14
Publisher: Kerbala University جامعة كربلاء

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Abstract

The study is designed to determine the differences between readings of hematocrit by the digital hematocrit reader and the standard centrifugation based hematocrit measuring instrument.75 individuals (25 healthy individuals, 25 anemic individuals and 25 polycythemic individuals), 38 males and 37 females with age range of 18-51 years, were studied after their consent. For each patient, 2 ml of venous blood sample was obtained for hematocrit measurement by the two different devices for comparison. Results showed that the digital hematocrit measures are less than that of the standard centrifugation based instrument in 69 out of 75 individuals i.e 92% which requires special attention during diagnosis or treatment and follow up.

تمت دراسة نسبة الدم باستخدام الجهاز الرقمي والجهاز القياسي المعتمد على الطرد المركزي للمقرنة بين القراءات لدى 75 شخصا (25 شخصا طبيعيا, 25 شخصا مصابا بفقر الدم و 25 شخصا مصابا بزيادة في نسبة الدم)وتم سحب 2 ملي لتر من الدم الوريدي بعد اخذ الموافقة منهم لقياس نسبة الدم بجهازين مختلفين. أظهرت النتائج بان نسبة الدم باستخدام الجهاز الرقمي اقل منها في الجهاز القياسي لدى 69 من مجموع 75 شخصا اي بنسبة 92% من الحالات وهذا يحتاج الى الانتباه الخاص عند تشخيص او متابعة علاج المريض ومعرفة او تحديد نوع الجهاز المستخدم لقياس نسبة الدم.

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Article
Activity assay of plasma fibrinogen in patients with diabetes.
تقييم نشاط الفايبرينوجين في بلازما الدم لدى المرضى المصابين بداء السكري

Author: Mohammed Shnain Ali محمد شنين علي
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2013 Issue: 6 Pages: 127-131
Publisher: Kerbala University جامعة كربلاء

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Abstract

Thrombosis secondary to atherosclerosis or hypercoagulability state in diabetes mellitus may be the cause of death in many patients. Patients with diabetes mellitus (n=28, 16 males and 12 females), together with 28 age and sex matched healthy individuals (as controls), were studied after their consent. For each individual (patients and controls), following medical history, 4 ml of venous blood sample was obtained and investigated for HbA1c level, fibrinogen level by doing Claus technique and blood sugar estimation. Hyperfibrinogenaemia in patients with diabetes mellitus (mean plasma fibrinogen 6.8±1.1) is statistically significant (P< 0.05) as compared to control (mean plasma fibrinogen 3.3±1.3). Plasma fibrinogen level is high in patients with diabetes mellitus, suggesting that plasma fibrinogen can contribute to vascular diseases in patients with diabetes mellitus like vascular thrombosis.

خثرة الدم بسبب تصلب الشرايين او بسبب زيادة قابلية الجسم للتخثر الدموي يمكن ان يكون سببا للوفاة في المرضى المصابين بداء السكري. تمت دراسة 28 شخصا (16 من الذكور ؛ 12 من الاناث) مع دراسة 28 شخصا من نفس الجنس والعمر من الاصحاء للمقارنة بعد اخذ الموافقة منهم. تم سحب 4 مليليتر من الدم الوريدي من كل شخص وتم اجراء الفحوصات المختبرية الخاصة بنسبة الهيموغلوبين نوع أي وان سي الخاص بنسبة السكر التراكمي في الدم بالطريقة الكيميائية وقياس نشاط او فعالية الفايبرينوجين بطريقة التخثر باستخدام مادة الثرمبين لاجراء الاختبار بطريقة كلاوس العالمية و قياس نسبة السكر في الدم بالطريقة الكيميائية.تبين ان نشاط او فعالية الفايبرينوجين في الدم عالية بفرق احصائي مهم لدى المرضى المصابين بداء السكري(6,8±1,1) مقارنة بالاصحاء(3,3±1,3) لذا فان نشاط او فعالية الفايبرينوجين عالية لدى مرضى داء السكري ويمكن ان يسبب او يساهم مع بقية العوامل في تخثر الدم في الاوعية الدموية.


Article
The Prevalence of Cases of B-Thalassemia Minor Among the Hematologically Suspected Cases in Kerbala.

Authors: Duha Maitham Hassan1 --- Mohammed Shnain Ali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2017 Volume: 10 Issue: 3 Pages: 3841-3845
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: B-thalassemia minor is the symptomless carrier state of B-thalassemia that can be misdiagnosed as mild iron deficiency anemia (IDA).Aim: This work aims to assess the prevalence of cases of B-thalassemia minor among the suspected cases and to compare the results of diagnosis depending on single confirmatory test (Hb electrophoresis) and three confirmatory tests (reticulocyte count, serum ferritin and Hb electrophoresis).Materials and methods: In this study 50 cases of mild anemia (Hb ≥9-11.99 g/dl) with initial hematological suspicion of B-thalassemia minor were studied for detailed confirmatory diagnostic tests that include reticulocyte count, Hb electrophoresis for HbA2 estimation and S.Ferritin level.Results: Results showed that 43/50 i.e 86% of suspected cases are confirmed by three tests (41/50 i.e (82%) have B-thalassemia minor, 2/50 i.e (4%) have B-thalassemia minor and IDA), 6/50 i.e (12%) have iron deficiency anemia (IDA), while 1/50 i.e (2%) have polycythemia (on venesections). Discussion: Results showed that 86% of initially suspected cases are confirmed to have B-thalassemia minor and 4% of cases have B-thalassemia minor with IDA depending on three tests for confirmatory diagnosis which are reticulocytosis, HbA2 concentration and S.Ferritin level. Similar results were encountered by Cristina Passarello, Antonino Giambona, et al.Conclusion: The prevalence of cases of B-thalassemia minor among the hematological suspected cases in Kerbala is 86%.

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