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Article
Immobilization of urease in gelatin beads for urea estimation

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Abstract

Abstract Urease was immobilized in gelatin beads. The optimum immobilization (79% activity) was observed at 4 C˚ with protein concentration of 1.0 mg/ml. the apparent optimum pH shifted from 7.5 to 6.5. Immobilized urease showed optimum activity at 60 C˚ compared with 40 C˚ for the soluble urease.The immobilized urease remained very active over long period of time and this enzyme lost about 40% of its original activity over the period of 60 days for storage at 4C compared with soluble urease which lost about 75% its activity in the same conditions. Blood urea estimation is carried out with immobilized enzyme beads compared with soluble enzyme and the beads can be used repeatedly for this purpose making it economical procedure compared to standard chemical method.

الخلاصةتم تقيد أنزيم اليوريز باستخدام الجيلاتين وكانت نسبة التقيد 79% اظهر الأنزيم المقيد أعلى فعالية عند رقم هيدروجيني 6.5 مقارنة بالأنزيم الحر7.5. واظهر أعلى فعالية عند درجة حرارة 60 ˚م مقارنة بالأنزيم الحر (40 ˚م).بقي الأنزيم المقيد فعال لفتره طويله حيث فقد حوالي 40% من فعاليته عند خزنه لمدة 60 يوم مقارنة بالأنزيم الحر الذي فقد 75% من فعاليته عند خزنه بنفس الظروف. تم تقدير نسبة اليوريا بالدم باستخدام الأنزيم المقيد وكانت الطريقة كفوءة واقتصادية.

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Article
Hormonal Contents of Two Types of Black Seed (Nigella sativa) Oil: Comparative Study

Authors: Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- Mufeed J. Ewadh مفيد جليل عوض --- Haider K. Zaidan حيدر كامل زيدان
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2006 Volume: 3 Issue: 1-2 Pages: 17-21
Publisher: Babylon University جامعة بابل

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Abstract

The use of black seed (Nigella sativa) capsules as a herbal therapy to lower blood glucose in diabetic patients are associated with menstrual cycle irregularities. The present study was designed to analyses and to compare between two types of Nigella sativa (N.sativa) agricultures in Mosul region and those imports from Saudi Arabia Kingdom, and to investigate the levels of hormones in volatile oil of black seed that regulate menstrual cycle that might be present in Nigella sativa. Volatile oil of N.sativa was extracted by steam distillation using diethyl ether as an organic solvent.Then, hormones analysis were done using (miniVIDAS software version) instrument. Results of present study showed a presence of considerable amount of sex hormones estradiol, progesterone, prolactin, testosterone, FSH and LH .This might interpret the cause of menstrual cycle irregularities occur when black seed capsule was taken as a herbal therapy to lower blood glucose in diabetic patients.

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Article
The Correlation between Lipid Profile and Lipid Peroxidation in Patients with Acute Myocardial Infraction

Authors: Mufeed J. Ewadh مفيد جليل عوض --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- Husain S. Aljanabi --- Alaa Hummady Alta’ee
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2005 Volume: 2 Issue: 3 Pages: 301-306
Publisher: Babylon University جامعة بابل

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Abstract

To measure the association between risk factor of cardiovascular disease (CVD) and lipid peroxidation in patients with acute myocardial infraction (AMI) and healthy controls, twenty six patients (17 males, 9 females) with AMI clinically diagnosed admitted to Merjan Teaching Hospital in Hilla city and fifty two (25 males ,27 females) apparently healthy persons as a controls subject to present study. Determination of serum total cholesterol (TC), triacylglycerol (TG), high density lipoprotein (HDL)-cholesterol, serum glutathione and serum lipid peroxidation were preformed using colorimetric methods. Very low density lipoprotein (VLDL) and low density lipoprotein (LDL) was determined using mathematically method. TC and TG of males with AMI found to be significantly increased and total cholesterol of females with AMI found to be increased when compared with healthy controls. HDL- cholesterol of patients found to be decreased, whereas risk factor was significantly increased. VLDL of patients with AMI found to be significantly decreased, whereas LDL found to be significantly increased. Lipid peroxidation was increased, but serum glutathione was declined significantly in patients with AMI when compared with healthy controls. The correlation between lipid peroxide and TG shows a positive relationship of oxidation product excretion at higher levels of TG. We conclude that patients with elevated levels of serum TG and cholesterol are at increased risk of oxidative damage due to lipid peroxidation.

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Article
Cytidine Deaminase Activity In Breast Cancer
فعالية السايتدين دي امينيز في سرطان الثدي

Authors: Oda M. Y. Al-Zamely --- Mufeed J Ewadh مفيد جليل عوض --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2004 Volume: 1 Issue: 1 Pages: 32-39
Publisher: Babylon University جامعة بابل

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Abstract

Cytidine deaminase (CTD) catalyzed the deamination of cytidine (CR) to uridine (UR) is a cytoplasmic enzyme existing in many kind of cells and widely distributed among mammalian tissues. It has beel1 reported to be a sensitive indicator in several types of abnormalities. CTD activities in sera of thirty-eight females with breast cancer (BC) and twenty-five healthy females asa contrcl were measued using a spectrophotometric method. Also electophoretic patte.n ofsera of patients with BC and healthy fernales were assayed using polyacrylamide gel electrophoresis(PAGE) to differentiate between normal and abnomal proteins existing in the two cases.Compared with healthy females, CDA activities found to significantly increase in sera of patients with BC (4.96+ 2.34, 13.3 + 3.72 U/L) (P < 0.000) respectively. PAGE profile of sera of patients with BC shows wide variety than that of nomal females.These results may consider a novel biochemical marker to aid in the diagnosis of BC.

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Article
The Key Role Correlation Of Creatine Kinase Activity and Antioxidants Status in Diabetic Patients Type I and II

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Abstract

AbstractThe activity of creatine Kinase (CK) estimated, in sera of diabetic patients (36 males, 18 females) type I & II and the concentration of antioxidant variables such as Glutathione, uric acid compared with healthy controls has been also estimated, CK activity was found to be significantly decrease in patients with Diabetes Mellitus; while isoenzyme (MM-CK,BB-CK and MB-CK) levels fluctuated between decrease and relatively constant in patients with Diabetes mellitus .Glutathione, uric acid was found to be reduced when compared with those of healthy controls. The depletion in antioxidant concentrations may be due to their protective role against oxidative stress in those patients

الخلاصةتم قياس فعالية أنزيم الكرياتين كاينيزفي أمصال مرضى داء السكري النوع الأول , والثاني (36 ذكور,18 إناث ) اضافه إلى قياس المتغيرات في تراكيز مضادات الاكسده كالكلوتاثايون وحامض اليوريك لتبيان العلاقة بين الطرفين وقد دلت النتائج بان فعالية الإنزيم ومضادات الاكسده تناقصت بشكل ملحوظ في مرضى السكري بينما أظهرت متماثلات الإنزيم تغيرات تراوحت بين ثبوت ونقصان عند نفس المرضى .إن النقصان الحاصل في تراكيز المضادات قد يعود إلى قابليتها على مقاومه الجهد التاكسدي الحاصل في هؤلاء المرضى وبذلك هدفت الدراسة إلى خلق علاقة مهمة بين الإنزيم من جهة وبين المضادات من جهة أخرى للاستدلال على القيم المعنوية لهذه العلاقة البايولوجيه.

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Article
Lipid profile in Diabetic Children

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Abstract

AbstractHeart problems are an important cause of morbidity and mortality all over the world. High cholesterol is an important cause which might start in childhood The aim of this study is to assess lipid profile as a risk factor for heart disease. Fifty two children with diabetes mellitus type 1 were assessed regarding their lipid profile compared to 37 non diabetic children. The results shows that growth parameters were found lagging behind the normal range for age and sex.Cholesterol and LDL(Low Density Lipoprotein) were found high compared to control group(P<0 .05).

الخلاصةتعتبر أمراض القلب من الأسباب المهمة للوفاة في العالم ويعتبر ارتفاع نسبة الدهون عاملا مهما في ذلك وقد تبدأ الزيادة في الدهون في عمر الطفولة. هدف البحث هو لقياس نسبة الدهون في الأطفال المصابين بالسكر كعامل مؤدي إلى تصلب الشرايين. تمت دراسة اثنين و خمسين طفلا مصابا بالسكر المعتمد على الأنسولين و قياس نسبة الكولسترول و الدهون قليلة الكثافة و قورنت النتائج مع سبع و ثلاثين عينة سيطرة لأطفال أصحاء و كانت الفروقات معنوية(P<0.05)

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Article
The Behavior of the Plasma Homocysteine and Selenium Concentrations in Patients with Acute Myocardial Infarction
سلوك مستويات الهوموسيستين والسيلينيوم في بلازما دم المصابين باحتشاء العضلة القلبية الحاد

Authors: H. Renz --- S. Mkhlof --- Aamir S. AL-Mu'min --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- et al.
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2008 Volume: 5 Issue: 3-4 Pages: 543-550
Publisher: Babylon University جامعة بابل

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Abstract

Background: Increased concentrations of plasma total homocysteine and decreased concentrations of plasma selenium are separately associated with cardiovascular disease. Objective: Investigate the correlation between plasma total homocysteine and selenium in patients with acute myocardial infarction (AMI). Methods: Patients of present study was thirty nine men with AMI admitted to Marburg Hospital in Marburg city, Germany on 2007. Plasma total homocysteine was determined using HPLC. Plasma selenium was determined using atomic absorption spectrometry. Results: Plasma homocysteine of patients found to be increased, whereas plasma selenium found to be decreased when compared with reference range . The correlation between plasma homocysteine and selenium found to be negatively correlated. Conclusion: The negative correlation between plasma tHcy and selenium may indicate that they have a significant impact on the process of atherogenesis. The change in the levels of plasma total homocysteine and plasma selenium might be result from oxidative stress associated with AMI.

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Article
The Effect of H2O2 and Some Antioxidants on Human Sperm Parameters in vitro

Authors: Faris, N.A.A1-Hady فارس ناجي عبود --- Sahib, Y.H.Al-Morshidy صاحب يحي المرشدي --- Mufeed, J.Ewadh مفيد جليل عوض
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2012 Volume: 20 Issue: 5 Pages: 1414-1418
Publisher: Babylon University جامعة بابل

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Abstract

This study was performed to determine the effect of H2O2 addition to the semen specimens to stimulate the formation of free radicals, and treated with antioxidants. This study involved ten semen specimens collected from fertile men. Seminal fluid analysis was performed to estimation sperm parameters and Malondialdehyde (MDA) concentration. Also seminal fluid analysis and MDA level was determination after adding of H2O2 and then antioxidants (VE, VC, VE+VC, glutathione and seminal plasma).The results revealed a significant decrease (p<0.05) in sperm motility percent, grade activity and sperm viability percent, and significant increase (p<0.05) in MDA concentration by addition 0.1% of H2O2 to semen specimens compared to those values with normal saline. The addition of antioxidants (0.6mg/ml VE, 0.4mg/ml Vc + 0.6mg/ml VE, 0.4rng/ml glutathione and seminal plasma) to the semen specimens contain 0.1% H2O2 caused significant increase(p<0.05) in sperm motility percent, grade activity and sperm viability percent , and significant decrease (p<0.05) in MDA level compared to those values in semen samples contain 0.1% H202 alone. While VC caused insignificant differences (p>0.05) in all sperm parameters and MDA concentration.

اجريت هذه الدراسة لتحديد تأثير اضافة بيروكسيد الهيدروجين الى عينات السائل المنوي لحث انتاج الجذور الحرة ومن ثم اضافة مضادات الاكسدة لتخفيف ضرر الجذور الحرة. شملت هذه الدراسة عشر عينات مني جمعت من اشخاص خصيبين. تم اجراء فحص السائل المنوي لحساب معالم النطف بالاضافة لقياس تركيز المالونداي الديهايد. كذلك فقد اجريت الفحوصات نفسها بعد اضافة 0.1% بيروكسيد الهيدروجين لوحده وكذلك بعد اضافة مضادات الاكسدة (فيتامين E وفيتامين C وفيتامين +C فيتامين E والكلوتاثيون والبلازما المنوية) الى عينات المني الحاوية على بيروكسيد الهيدروجين.اشارت النتائج حصول انخفاض معنوي (P<0.05) في كل من النسبة المئوية للنطف المتحركة ودرجة نشاط النطف والنسبة المئوية لحيوية النطف وزيادة معنوية (P<0.05) في تركيز المالونداي الديهايد لدى اضافة OH بيروكسيد الهيدروجين مقارنة بقيمها في عينات المني المضاف اليها المحلول الفسيولوجي.سببت اضافة اضافات مضادات الاكسدة (0.6ملغم/مل فيتامين E و 0.4 ملغم/مل فيتامين C +0.6 ملغم/مل فيتامن E و 0.4 ملغم/مل كلوتاثيون والبلازما المنوية) الى عينات المني الحاوية على بيروكسيد الهيدروجين حصول زيادة معنوية (P<0.05) في كل من النسبة المئوية للنطف المتحركة ودرجة نشاط النطف والنسبة المئوية لحيوية النطف ونقص معنوي (P<0.05) في تركيز المالونداي الديهايد مقارنة بقيمها في عينات المني الحاوية على بيروكسيد الهيدروجين لوحده. اما فيتامين C فلم يظهر أي فرق معنوي في معالم النطف كافة وكذلك مستوى المالونداي الديهايد.

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Article
Relationship between Lipid Profile and Renal Function Parameters in Patients with Acute Myocardial Infarction

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AbstractBackground: Cardiovascular disease(CVD) are related to multiple metabolic risk factor as hypertension , diabetes mellitus, hyperlipidaemia and cystiene level, as well. Objective: To investigate the association between lipid profile as risk index for CVD and renal function parameters in patients with acute myocardial infarction (AMI). Methods: One hundred and one patients (51 males, 50 females) with AMI admitted to Merjan Teaching Hospital in Hilla city on 2007 and fifty five (30 males, 25 females) apparently healthy persons as a controls subject to present study. Determination of blood Total cholesterol (TC), triacylglycerol (TG), high density lipoprotein (HDL)-cholesterol, serum creatinine (Cr) and serum uric acid (UA) were preformed using colorimetric methods. Very low density lipoprotein (VLDL)-cholesterol and low density lipoprotein (LDL) -cholesterol was determined using mathematical method. Risk index of lipid profile determined by dividing TC/ LDL –cholesterol. Results: Serum Cr and UA found to be significantly increased in patients with AMI when compared with healthy controls. Total cholesterol , VLDL- cholesterol, LDL- cholesterol and TG of patient with AMI in both gander found to be significantly increase when compared with healthy controls. HDL- cholesterol of patients found to be decreased. Conclusion: Serum Cr and serum UA can be regarded as risk factor for patient with CVD.

الخلاصةترتبط الأمراض القلبية الوعائية بالعديد من العوامل المخطرة الايضية كارتفاع ضغط الدم وداء السكري ووفرة الدهون في الدم ومستويات السستين. تهدف هذه الدراسة لتقصي العلاقة بين معالم الدهون كعوامل مخطرة للأمراض القلبية الوعائية ومتغيرات وظائف الكلية عند المرضى المصابين بأحتشاء العضلة القلبية.شملت هذه الدراسة مائة وواحد مريضا مصابا بأحتشاء العضلة القلبية (51 ذكر و50 أنثى) من الداخلين إلى مستشفى مرجان التعليمي في مدينة الحلة خلال العام 2007 ،وكذلك شملت خمسة وخمسون (30 ذكر و25 أنثى) شخصا سليما كمجموعة سيطرة. تم تقدير كل من الكولسترول الكلي (TC) في الدم والكليسرايد ثلاثي الأسيل (TG) وكولسترول البروتين الدهني عالي الكثافة (HDL) وكرياتنين وحامض يوريك المصل بطريقة لونية. بينما قدر كل من كولسترول البروتين الدهني واطئ الكثافة (LDL)و كولسترول البروتين الدهني واطئ الكثافة جدا (VLDL)بطريقة حسابية. كما قدر عامل الخطورة بقسمة الكولسترول الكلي/ كولسترول البروتين الدهني واطئ الكثافة. أظهرت النتائج ارتفاعا معنويا في كل من تركيز حامض اليوريك ،والكرياتنين، وكولسترول البروتين الدهني واطئ الكثافة، و كولسترول البروتين الدهني واطئ الكثافة جدا ،والكليسرايد ثلاثي الأسيل ،في حين انخفض تركيز كولسترول البروتين الدهني عالي الكثافة ،عند المرضى مقارنة بمجموع السيطرة.تستنتج الدراسة إمكانية اعتبار كرياتنين وحامض يوريك المصل كعوامل مخطرة عند المرضى المصابين بالأمراض القلبية الوعائية.

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Article
Diabetes Mellitus Correlation with Free Radicals and Creatine Kinase Isoenzymes Activity

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To explain the relationship between free radicals production in experimentally diabetic animals and expected glutathione (GSH) reducing levels with its enhancement due to feeding of cysteine, N-acetyl cysteine and to evaluate the action of antioxidant to relief the damage to the active site of creatine kinase (CK) caused by free radicals, three doses during 48 hours of 150 mg alloxan / kg body weight was injected to forty eight males albino mice to produce the diabetes mellitus. Blood glucose and serum glutathione were determined using colorimetric methods, whereas, fractionation of CK isoenzymes in sera of diabetic mice was conducted by mini column ion exchange chromatography using Sephadex A-50. Agarose gel electrophoresis was used to quantitize the CK isoenzymes. Results of present study show that after long period of induced diabets in males albino mice cause a change in CK isoenzyme distribution pattern The total serum CK activity decreased in 75% of animals and reached to 63.5% of the control value whereas CK - MM isoenzyme was also decreased to 54.1% of its initial activity. Other isoenzymes showed variation depending upon the period of diabetes induced which caused elevated levels after 30 days of CK -BB isoenzyme in 62.5% of the animal studied and reached to 68% more than its initial activity which in < 3%. CK - MB which is found in 25% of albino mice was elevated to 17.1% more than that found in control sera which is < 3%. Insulin affects total CK activity and its isoenzymes distribution pattern as a therapeutic agent, and CK activity found to be elevated Thiol containing compounds including cysteine, N-acetyl cysteine dithiothreitol and glutathione have been suggested to reactivate the CK activity.

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