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Article
Exogenous melatonin induces histological changes in collecting tubules and ducts of male rat's kidney

Author: Mustafa M. Ibraheem
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2006 Volume: 6 Issue: 2 Pages: 16-21
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Selective re-absorption and secretion are the functions of the collecting tubules and ducts, in addition to concentrate urine through ADH-regulated and ADH- independent water channels. In this study, twenty four male rats were used; they were divided into two groups of animals: Group (A) included twelve rats of five weeks old age (before puberty) that were divided into three subgroups, four rats in each subgroup. Subgroup I was control one, subgroups II and III were treated orally with melatonin in a dose of 250 & 500 μg/kg body weights subsequently. Group (B) included twelve rats of seventeen weeks old age (after puberty) that were divided into the same subgroups and treated with the doses of melatonin as in the rats of group (A). In this experiment, the exogenous melatonin induced structural changes on the collecting tubules and ducts, in dose dependent manner as well as onset-dependent manner whether used in pre- or post-puberty of male albino rats


Article
IMMUNOHISTOCHEMICAL EXPRESSION OF STEM CELL MARKERS CD44, CD166 AND ALDH1A1 IN NON-NEOPLASTIC POLYPS OF THE COLON IN SAMPLE OF IRAQI PATIENTS

Authors: Basim Sh Ahmed --- Mustafa M Ibraheem --- Nawal M Abdullah
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2017 Volume: 23 Issue: 1 Pages: 37-44
Publisher: Basrah University جامعة البصرة

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Abstract

IMMUNOHISTOCHEMICAL EXPRESSION OF STEM CELL MARKERS CD44, CD166 AND ALDH1A1 IN NON-NEOPLASTIC POLYPS OF THE COLON IN SAMPLE OF IRAQI PATIENTSNawal M Abdullah*, Mustafa M Ibraheem@ & Basim Sh Ahmed#*MB,ChB, MSc, Assist. Prof., Department of Anatomy, College of Medicine, University of Basrah. @MB,ChB, MSc, PhD, Assist. Prof., Department of Anatomy, College of Medicine, University of Al-Mustansiryah. #MB,ChB, FICMS, Assist. Prof. Department of Pathology, College of Medicine, University of Al-Mustansiryah, IRAQ.Abstract The aim of this retrospective study on tissue specimens of benign colorectal polyps is to evaluate the significance of IHC markers CD44, CD 166 and ALDH1A1 expression in non-neoplastic (Juvenile and Hamartomatous) polyps and their association with different clinicopathological parameters & to compare their expression in benign polyps with that of normal colonic tissue . Seventy cases enrolled in the study including benign polyps (juvenile & hamartomous polyps) & benign looking colonic tissue obtained from archive of histopathology unit in Gastroenterology and Hepatology hospital in Baghdad Medical City and histopathology unit in Al-Sadder Teaching Hospital in Basrah city for the period Sept. 2015 to Dec. 2016. Four micrometer sections were obtained from formalin fixed paraffin-embedded blocks treated IHC with CD44, CD166 and ALDH1A1 tumor markers. Majority of polyps were juvenile, no difference regarding gender distribution. The common location was rectum/rectosigmoidal region. CD166, CD44 and ALDH1A1 showed high expression regarding age, gender and location in benign tumors when compared with healthy looking tissue. In conclusion, colorectal polyps are critical clinical entity and many of them are a precursors to malignant diseases so colonoscopy is essential screening test, polypectomy and follow up is mandatory for patients with colorectal polyps even if they are benign.


Article
Comparative Study Between Therapeutic Effects of Melatonin and Zinc in Lead induced Toxicity in Rats

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Abstract

The present study was designed to investigate the therapeutic effects of melatonin as well as zinc in combination or each one alone against the hepatic and renal toxicity of lead in male rats. Five groups of animals were allocated as follows: Group I, includes 12 rats injected subcutaneously with 0.2 ml physiological saline for 60 days; groups II and III, each includes 12 rats, injected with lead acetate 100 mg/kg/day s.c for 30 days, followed by treatment with intraperotoneal injection of physiological saline (0.2 ml) or melatonin 30mg/kg/day for the next 30 days. Group IV, include 12 rats, injected with lead acetate 100 mg/kg/day s.c for 30 days, followed by treatment with intraperotoneal injection of zinc sulphate 1.5 mg/kg/day for the next 30 days. Group V, includes 12 rats injected with 100mg/kg lead acetate s.c for 30 days followed by treatment with intraperitoneal injection of 30 mg/kg/day melatonin and 1.5mg/kg/day zinc sulphate for the latter 30 days. At the end of treatment period, the rats were sacrificed by an overdose (100mg/kg) of thiopental (twenty-four hour after the last injection). Laparotomies were performed to obtain the livers and kidneys for the assessment of tissue damage. Oxidative stress parameter (MDA), hemoglobin content, liver and renal functions, and histological appearance of the studied organs were evaluated and compared with that of negative and positive controls. Treatment with melatonin or zinc reverses the damage induced by lead in many organs and tissues through the reduction of MDA levels in RBCs, liver and kidneys; in addition to the improvement in the indices of the functions of the organs studied. These findings demonstrated that addition of zinc to melatonin are capable of further reversing damage of rat tissues caused by successive doses of lead acetate, and animals had restored their organ functions due to combined treatment.

صممت هذه الدراسة لتحديد التأثيرات العلاجية للميلاتونين وكذلك الزنك, أما كل على حدا أو بصورة مجتمعة على التأثيرات السمية بواسطة الرصاص على الكبد والكلية عند الجرذان الذكور.تم تعريض مجاميع الحيوانات الخمسة التي تمت دراستها إلى جرعة يومية مقدارها 100ملغم/كغم من مادة خلات الرصاص تحت الجلد لمدة شهر واحد. تم استخدام (30ملغم /كغم) من مادة الميلاتونين او الزنك عن طريق البريتون بعد استمرار التسمم للرصاص لمدة شهر آخر. تمت متابعة التغيرات الحاصلة في معايير فرط الأكسدة ، محتوى الدم من الهيموغلوبين ،فعالية الكبد والكليتين إضافة إلى التغييرات النسيجية التي طرأت على الأعضاء التي تمت دراستها مقارنة مع المجموعة القياسية (السلبية والايجابية). يمكن الأستنتاج من هذه النتائج أن الميلاتونين والزنك لهم القابلية على منع الضرر النسيجي نتيجة للتعرض الى جرع متعاقبة من خلات الرصاص وأن الحيوانات المختبرية لها القدرة على أستعادة فعالية أعضاءها الحيوية نتيجة للعلاج بالميلاتونين والزنك معا بعد التعرض للرصاص.

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