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Article
Effect Nigella Sativa (Black Seed) on Liver Enzymes in Ischemia Reperfusion Injury

Author: Mustafa Taha Mohammed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: supplement Pages: 654-657
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Hepatic ischemia-reperfusion (I/R) injury may occur in a variety of clinical settings and this remains a significant problem. Oxygen free radicals, produced on reperfusion have been shown to play a major role in hepatic I/R injury. Various therapeutic effects have been described for Nigella sativa. Additionally, it has been presented that Nigella sativa (orientalis) oil has protective effect against ischemia reperfusion injury to various organs. Therefore, it seems possible that the administration of Nigella sativa (orientalis) oil might protect the liver against the ischemia reperfusion injury . OBJECTIVE: To determine whether Nigella sativa (orientalis) oil prevents hepatic ischemia-reperfusion injury to the liver. METHODS: Thirty-six rats were divided into three groups as control (Group 1), I/R group (Group 2), and Nigella sativa oil (NS) treatment group (Group 3). All rats underwent hepatic ischemia for 60 min followed by 60 min period of reperfusion. Rats were intraperitoneally infused with only 0.9% saline solution in group 2. Rats in group 3 received NS oil (0.2 mL/kg) intraperitoneally, before ischemia and before reperfusion. Blood samples were harvested from the rats, and then the rats were sacrificed. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were determined. RESULTS: The levels of liver enzymes in group 3 were significantly lower than those in the group 2. CONCLUSION: Our results suggest that Nigella sativa oil treatment protects the rat liver against to hepatic ischemia-reperfusion injury.


Article
Effect of Trigonella Foenum-Graecum L. (Fenugreek) on Liver Enzymes in Ischemia-Reperfusion Injury

Author: Mustafa Taha Mohammed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 1 Pages: 69-74
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Hepatic ischemia-reperfusion (I/R) injury may occur in a variety of clinical set¬tings and this remains a significant problem. Oxygen free radi¬cals, produced on reperfusion have been shown to play a major role in hepatic I/R injury. Various therapeutic effects have been described for Trigonella. Additionally, it has been presented that Trigonella has protective effect against ischemia reperfusion injury to various organs. Therefore, it seems possible that the administration of Trigonella might protect the liver against the ischemia reperfusion injury.OBJECTIVE:To determine whether Trigonella foenum-graecum L. (Fenugreek) prevents hepatic ischemia-reperfusion injury to the liver.METHODS: Thirty-six rats were divided into three groups as, (Group 1) control group, (Group 2)I/R group and (Group3) Trigonella foenum-graecum L. (Fenugreek) treatment group. All rats underwent hepatic ischemia for 60 min followed by 60 min period of reperfusion. Rats were internal infused with only 0.9% saline solution in group 2. Rats in group 3 received hydro alcoholic - extracted Trigonella foenum-graecum L. (Fenugreek) (500 mg/kg)internal, before isch¬emia and before reperfusion. Blood samples were harvested from the rats, and then the rats were sacrificed. Serum aspartate aminotransfera¬se (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were determined.RESULTS:The levels of liver enzymes in group 3 were significantly lower than those in the group 2.CONCLUSION: Our results suggest that Trigonella foenum-graecum L. (Fenugreek) treatment protects the rat liver against hepatic ischemia-reperfusion injury


Article
Study of Some Mirabilis jalapa L. Leaves Components and Effect of Their Extracts on Growth of Pathogenic bacteria

Author: Mustafa Taha Mohammed
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2012 Volume: 23 Issue: 6 Pages: 117-124
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The chemical components of the Mirabilis jalapa L. leaves in the watery and alcoholic extracts were identified .The results showed that the extract contain : glycosides ,tannins ,phenolic compounds , resins ,alkaloids and proteins ,while the saponins and flavonoids were not found. The results also showed that there were concentrations of the following trace elements in the leaves K , Na , Fe with 161.2 ,19 ,18.7 µg/ml , respectively and concentrations of Zn ,Ca with 14.2 , 12 µg/ml respectively , concentrations Cd ,Cu, Pb with 0.8 ,0.3,0.1 µg/ml respectively ,and Cr were not founds. The effects of these extracts on growth of different bacteria were studied, has been found that 0.5 mg/ml concentration was effective inhibitor of growth of Staphylocoous aureus , E.coli and Proteus mirabilis

شملت الدراسة معرفة المكونات الكيميائية الفعّالة الموجودة في أوراق نبات لالة عباس Mirabilis jalapa, إذ أظهرت الدراسة أن محلولي المستخلصين المائي والكحولي يحتويان على مجموعة من المركبات الكلايكوسيدية والعفصية والفينولية والراتنجات والقلويدات والبروتينات بينما لا تحتوي على الصابونيات والفلافونويدات . اثبت التحليل الدقيق للعناصر المعدنية لأوراق النبات احتواءها على تراكيز من Kو NaوFe وهي161.2 ,19 18.7 µg/ml على التوالي ، وكميات من Zn وCa وهي 12, 14.2µg/ml وكميات على Cd, Cu وPb وهي 0.8 ,0.3,0.1 µg/ml . وعلى التوالي, وعدم احتوائها على Cr. كما درس تأثير المستخلصات المائية والكحولية على أنواع مختلفة من البكتريا أذ لوحظ أن للتركيز 0.5 ملغم /مل تأثيرا فعّالا تجاه تثبيط نمو بكتريا Proteus mirabilis و E.coli و Staphylocoous aureus خاصة تجاه المستخلص الكحولي

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Article
Study of Some Miswak (Salvadora persica L) Components and Effect of Their Aqueous Extract on Antioxidant

Author: Mustafa Taha Mohammed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 1 Pages: 55-60
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Oxidative stress defines that, the level of Reactive Oxygen Species (ROS) exists in excess of antioxidant defenses. This imbalance in the redox milieu results in a switch from ROS-stimulated ambient signaling processes to ROS-mediated pathophysiological consequences. Oxidative stress has been implicated in the installation and progression of several degenerative diseases via DNA mutation, protein oxidation and/or lipid peroxidation. Therefore, possible use aqueous extracts of Miswak to protect brain against the Lipid peroxidation.OBJECTIVE: The present study was undertaken to evaluate the potential of Salvadora persica L. (miswak) against the DPPH Free Radical Scavenging System and Lipid peroxidation.METHODS: The chemical components of the prepared aqueous extracts of Miswak were detected as: glycosides, alkaloids, saponins, phenolic compounds, tannins, flavonoids , proteins ,steroids and Vitamine C. and then estimate DPPH Free Radical Scavenging System and Lipid peroxidationRESULTS: The study showed that the Miswak ( Salvadora persica L.) in the aqueous extracts contain : glycosides , proteins, saponins ,tannins ,phenolic compounds, flavonoids , alkaloids , steroids and vitamine C . Aqueas extracts were found effective in scavenging DPPH(62.45℅) in concentration (250µl/ml), as well as inhibiting the lipid peroxidation (42.04℅). CONCLUSION: Our results suggest that Miswak ( Salvadora persica L.) treatment protects the rat brain against lipid peroxidation and DPPH free radical scavenging..


Article
Antioxidant and Anti-Inflammatory Effect of Fruit Juice of Annona Muricata L (Soursop) During Ischemia Reperfusion Injury in Rats

Authors: Mustafa Taha Mohammed --- Sarah Isam Abbas
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 1 Pages: 118-123
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:OBJECTIVE:The study included knowledge The chemical components of the Soursop juice and determine whether Soursop prevents ischemia-reperfusion induced brain injuries in rats.METHODS:The chemical components of the prepared fruit extract were detectedits components of glycosides, proteins....etc and we were Determination of trace element and the estimation of the biochemical parameters: Blood samples were harvested from the rats,. Serum IL-10,Adiponectin and oxidative stress levels of Malondialdehyde ( MDA) and Glutathione ( GSH )were estimated.RESULTS: The results showed that the extract contain : glycosides, proteins, saponins, tannins, various phenolic compounds alkaloids , flavonoids, steroids and vitamine C . The results also showed that there were high concentrations of B, Fe , Zn respectively and low concentrations of Mn, Cu, pb,Cd respectively ,very low concentrations Ni ,Co , Se . The levels of oxidative enzymes and Inflammatory cytokines in group 3 were significantly lower than those in the group 2.Our results suggest that soursop treatment protects the rat brain against ischemia-reperfusion induced brain injuries.CONCLUSION: The present study confirm that the fruit extracts of Soursop (Annona Muricata L.) posses in vivo anti-inflammatory and antioxidant activity because of its content (glycosides , tannins , saponins ,proteins ,various phenolic compounds ,alkaloids , flavonoids, steroids and vitamine C )


Article
Identification of Some Annona Muricata L.(Soursop) Components and Their Antioxidant Effects in Rats

Author: Sarah Isam Abbas, Mustafa Taha Mohammed, Rehab Abd Al-Mahdi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2015 Volume: 14 Issue: 4 Pages: 576-580
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Oxidative stress defines that, the level of Reactive Oxygen Species (ROS) exists in excess of antioxidant defenses. This imbalance in the redox milieu results in a switch from ROS-stimulated ambient signaling processes to ROS-mediated pathophysiological consequences. Oxidative stress has been implicated in the installation and progression of several degenerative diseases via DNA mutation, protein oxidation and lipid peroxidation. Therefore, possible use of soursop fruit extract to protect brain against the Lipid peroxidation.OBJECTIVE: The present study was undertaken to evaluate the potential of Soursop (Annona Muricata L.) against the DPPH Free Radical Scavenging System and Lipid peroxidation.METHODS:Phytochemical screening was carried out with fruit extract of A. muricata for the detection of various phytochemicals. The extract was tested for the presence of glycosides, proteins, saponins, tannins, phenolic compounds , alkaloids , flavonoids, steroids and vitamine C using the standard procedures and then DPPH radical was estimated according to the method of Blois and Lipid peroxidation was estimated according to the method of Rajakumar.RESULTS: The study showed that the Soursop (Annona Muricata L.) in the fruit extract contain : glycosides , proteins, saponins ,tannins ,phenolic compounds, flavonoids , alkaloids , steroids and vitamine C . Fruit extract were found effective in scavenging DPPH (78,6℅) in concentration (250µl/ml), as well as inhibiting the lipid peroxidation (16.2℅). CONCLUSION: The results suggest that Soursop (Annona Muricata L.) treatment protects the rat brain against lipid peroxidation and DPPH free radical scavenging.

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