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Article
Detection of genomic instability in oral squamous cell carcinoma using random amplified polymorphic DNA based on polymerase chain reaction method (RAPD-PCR)

Authors: Shawki Shanan Abed شوقي شنان عبد --- Nadia S. Yass نادية ياس --- Majeed A. Sabah مجيد صباح
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 81-83
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral squamous cell carcinoma is an invasive epithelial neoplasm, occurred most commonly in alcoholicand tobacco using adults. The present study is aimed to identify the genomic instability in OSCC patients usingrandom amplified polymorphic DNA (RAPD), a polymerase chain reaction (PCR) based technique.Materials and methods: Twenty five blocks of formalin fixed paraffin embedded tissue was used as malignant DNAsource and five sample of healthy DNA obtained from the oral tissue and blood. Using DNA extraction kit (Geneaidminikit) and eleven random sequencing primers to visualize the amplifications pattern under UV.Results: The primer detectability of genomic instability ranged from 21% in well differentiated OSCC to 68% in poorlydifferentiated OSCC. CasesT8 and T13 showed highest genomic instability (75%). The results determined numbers ofgenomic instabilities among OSCC patients by comparing the pattern of amplifications of the primers in bothmalignant and healthy DNA.Conclusions: High significance correlation between primers detection rate and histopathological grade of OSCC.Further larger studies are needed to: 1) Obtain RAPD markers useful for OSCC for early diagnosis; 2) investigatedifferent genes directly involved in the etiology of OSCC; 3) analyze chromosomal instability among OSCC patients


Article
Immunohistochemical evaluation of actin expression in basal cell carcinoma and oral squamous cell carcinoma

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Abstract

Background: Basal cell carcinomas (BCCs) are generally slow-growing tumours. They have been classified as aggressive (A-BCC) and non-aggressive (NA-BCC).Oral squamous cell carcinoma (OSCC) is a major cause of cancer morbidity worldwide, this is due to the characteristics of invasion. The microenviroment or stroma of neoplastic tissues plays an active role in tumour progression. Trans-differentiation of fibroblast to myofibroblast is a crucial and early event in tumourigensis. Alterations of contractile tension generated by the actin–myosin complex are of central importance in the development of the phenotype of morphologically transformed neoplastic cells with invasive behavior. Actin is the predominant component of contractile microfillament and it may be associated with increase contractility and invasiveness of tumour cells.Objective: This study aimed to investigate the presence of myofibroblasts in the stroma of basal cell carcinoma and oral squamous cell carcinoma, evaluated by the immunohistochemical expression of actin.Materials and methods: Twenty four formalin –fixed, paraffin -embedded tissue blocks (14 cases basal cell carcinoma, 10 cases oral squamous cell carcinoma) were included in this study. An immunohistochemical analysis was performed using anti alpha - smooth muscle actin (α- SMA) monoclonal antibody.

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