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Article
Inhibitory effect of honey on some bacterial infections

Author: Najla'a Nabhan Yassein
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 3 Pages: 227-241
Publisher: Diyala University جامعة ديالى

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Abstract

The antibacterial profiles of honey were examined against clinical isolates of
Staphyllococcus aureus and Pseudomonas aeruginosa. Agar well diffusion method was used
in the antibacterial susceptibility studies. This study revealed that the honey obtained from
Agricultural college of Baghdad University was effective against bacteria.The highest
inhibitory zone of S. aureus reached to 20mm in diameter at 200 mg/ml of honey
concentration but not affected on P. aeruginosa. Wounds were done in male mice by using
shaver and then infected with 0.1ml of both bacterial suspensions with concentration 108
CFU/ml.Symptomes of infections were appeared after 24-48 hours. Three concentrations of
honey were used (100,200 and 300) mg/ml for treatment of skin infections by using 0.1 ml of
honey,also gentamycin ointment and normal saline were used. Symptomes of recovery based
on wound healing were noticed after 10,6,9,11 and 15 days for S. aureus and 12,8,13,15 and
18 days for P. aeruginosa when treated with 100,200 and 300mg/ml of honey,gentamycin and
normal saline respectively. 200 mg/ml of honey concentration showed the best concentration
for skin infection treatment. This study,therefore, suggest that honey could have strong
biocidal effect against S. aureus (both in vitro and in vivo) and against P. aeruginosa (in
vivo),therefore,have the potential effective role in the treatment of skin infection.


Article
Antibacterial effect of date palm (Phoenix dactylifera L.) pit aqueous extract on some bacteria cause urinary tract infection
تأثیر المستخلص المائي لنوى التمر على بعض أنواع البكتریا المسببة لالتھاب المجار ي البولیة

Author: Najla'a Nabhan Yassein نجلاء نبھان یاسین
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2012 Volume: 8 Issue: 3 - part 1 Pages: 112-120
Publisher: Diyala University جامعة ديالى

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Abstract

The present work was carried out to study the impact of date palm pit aqueous extract as an antibacterial on four species of pathogenic bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa). Four concentrations of date palm pit aqueous extract were prepared by using normal saline: 200,100,50 and25(mg/ml).200 and 100(mg/ml) were the most effective concentrations on the tested bacteria except K. pneumoniae which showed full resistance to all extract concentrations. Date palm pit extract was somewhat more effective in inhibiting growth of bacteria as compared with antibiotics includes trimethoprime, gentamycin and rifampin.

اجریت ھذه الدراسة لغرض دراسة تأثیر المستخلص المائي لنوى التمر كمضاد بكتیري على اربع انواع بكتیریة ممرضة ومسببة لالتھاب المجاري البولیة وھي:Proteus و Klebsiella pneumoniae و Escherichia coli تم تحضیر أربع تراكیز من المستخلص المائي لنوى التمر بإستعمال .Pseudomonas aeruginosa و vulgaris25 (ملغم/مل).أظھرت النتائج أن التراكیز 200 و 100 (ملغم/مل) كانت ،50 ، 100 ، المحلول الملحي الفسلجي وھي: 200 أظھرت مقاومة كاملة لكل تراكیز K. pneumonia الأكثر فعالیة في تثبیط البكتریا قید الدراسة، في حین أن بكتریاالمستخلص المائي لنوى التمر. كان المستخلص المائي لنوى التمر الى حد ما اكثر فعالیة في تثبیط البكتریا قید الدراسة عند مقارنتھ مع فعالیة المضادات الحیویة والتي تشمل المیثوبریم، الجنتامایسین والریفامبسین.

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