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Article
Prevalence and Molecular Characterization of Fim H Gene in Escherichia Coli Isolates Recovered From Patients With Utis

Author: Narmin Saeed Merza
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 470 -477
Publisher: Babylon University جامعة بابل

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Abstract

In this study, the prevalence of fim H gene was studied among 105 E. coli isolates obtained from urine samples of patients attended Azadi hospital in Duhok City. The intended gene was detected in94.3% of the isolates. Triplex PCR assay was applied and according to which the studied isolates were assigned into four groups namely A, B1, B2, and D groups which constituted 20.95 %, 3.8 %, 54.28 %, 20.95 %, respectively. Ten randomly selected isolates were subjected to SNPs fimH analysis with 3 reference strains of E. coli. The results revealed that 44 SNPs observed at 42 polymorphic sites accounting for 5.59%. All mutations were of substitutions and 29.5 % of mutations were transversions while transition type mutations constituted of 70.5 %. Ten SNPs accounting for 22.7 % of mutations gave rise to amino-acid changes (sense mutation) while the rest 34 (77.3 %) resulted in silent mutations. Moreover, twelve SNPs were singletons and among them five were with amino acid replacements. Amino acid replacements due to SNPs accounted for 1.27% of whole sequenced fragment of fimH.It can be concluded that there is no relationship inferred between the isolates of E.coli when the two phylotyping techniques are compared but the results of both can serve the purpose of genotypic characterization of uropathogenic E. coli.


Article
Molecular differentiation and determination of multi-drug resistant isolates of Pseudomonas species collected from burn patients in Kurdistan Region, Iraq
التفريق الجزيئي وتحديد العزلات المقاومة للعديد من أنواع Pseudomonas المجمعة من مرضى الحروق في إقليم كوردستان العراق

Authors: Jaladet M.S. Jubrael --- Marwan Khalil Qader --- Haval Mohammed Khalid --- Rana Adel Hanoon --- et al.
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2018 Volume: 22 Issue: 3 Pages: 394-400
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportunistic microorganism, a little challenge is being present with the identification of such pathogen. This study aimed to identify Pseudomonas on genus and species levels by conventional PCR and determine multi-drug resistant isolates.Methods: A total of 180 clinical isolates of Pseudomonas species were recovered from in and outpatients who attended Azadi and Rezgari Teaching hospitals in Duhok and Erbil city from October 2015 to May 2016. These isolates were phenotypically identified using standard microbiological procedures. A total of 100 isolates were randomly selected and confirmed at a molecular level as Pseudomonas spp.Results: By applying genus-specific gyr B2 primer which produced1130bp amplification band and sixty-eight isolates were identified by PCR as P. aueroginosa using species-specific primer for 16S rRNA region which showed 956bpamplicon. Forty-six isolates out of the sixty-eight resembling Pseudomonas aeruginosa were diagnosed as being multi-drug resistant isolates by the disc diffusion method.Conclusion: It can be concluded that multi-drug resistant isolates can pose a serious threat for the hospital-resident patients as increasing numbers of these isolates are being recorded in local settings.


Article
Molecular detection of methicillin resistant Staphylococcus aureus harbouring β- lactamase resistance genes isolated from different sources of infections
الكشف الجزيئي للمكورات العنقودية الذهبية المقاومة للميثيسيلين التي تؤوي جينات مقاومة اللاكتاماز المعزولة من مصادر مختلفة للعدوى

Authors: Zainab Hasan Abdullah --- Narmin Saeed Merza --- Ahmed Mohammed Sulaiman --- Amir Hani Raziq
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2017 Volume: 21 Issue: 3 Pages: 1889-1896
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: The detection and investigation of methicillin resistance staphylococci specifically S. aureus in clinical microbiology setting is very helpful both for informing the appropriate treatment of individual patients and also for the surveillance of these organisms. This study aimed at the rapid molecular detection of methicillin resistant staphylococci harbouring β-lactamase gene and determination of the efficiency of m-PCR through comparison with uniplex PCR.Methods: Standard microbiological techniques were applied for the determination of the presence of methicillin resistant S. aureus in samples recovered from different body sites of patients who attended Al-Kadhumyhia Teaching and Baghdad Teaching Hospitals. The resulting methicillin resistant S. aureus (MRSA) isolates were subjected to uni and multiplex PCR amplifications for detecting the existence of mec A gene and β-lactamase (TEM) resistance gene.Results: Half of the cases involved were found to be caused by MRSA. All the tested isolates showed positive amplification bands for the presence of mec A gene and only 48.8% of these harbored TEM gene. The obtained results revealed high sensitivity of universal bacterial and TEM primers expressed as 97.6% and 100% respectively, while the sensitivity of mec A primer was limited to 60%.Conclusion: The phenotypic identification of MRSA revealed a higher incidence rate and that different molecular techniques can yield conflicting results and it can also be concluded that resistant due to beta- lactamase production can be a crucial factor added to the previously settled methicillin resistant due to mec A gene.

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