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Cytotoxic effect of Azurin Produced from Pseudomonas aeruginosa Ps21 on cancer cell lines (Hep-2 and AMN-3)
التأثير السمي للأزورين المنتج من بكترياpseudomonas aeruginosa PS 21 في الخطوط الخلوية السرطانية Hep-3 و 2 AMN-

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Abstract

This stady was amied to detection the ability of azurin , produced from Pseudomonas aeruginosa Ps21, as inhibitortwo types of cancer cell lines, human laryux epidermoid carcinoma ( Hep – 2 ) and Mouse Mammary adenocarcinoma(AMN – 3 ) by using cytotoxicity assay.The Results showed the effect of partial purified of azurin depended on the concentration , type of cell and the exposuretime. The extract at high concentrations ( 125 , 250 ) μg/ml. were exhibited apotent inhibiory at all exposure timeagainst cancer cell lines. While The inhibitory activity was gradually reduced with decreasing of concentration ofazurin , the low concentrations were activated for the cells proliferation in comparison with the control (100 %).The viability of Hep – 2 cells was increased only during the first 24 hrfrom exposure while the AMN – 3 cells were proliferated at all exposture times (24 , 48 , 72) hr.

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