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Article
Oxidative Stress and Antioxidant Defense in Patients With Jaundice

Authors: Noaman Abdulateef Abdulrazzaq --- Perry Habib Saifullah --- Suhayla Khalied Mohammed3
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2014 Volume: 7 Issue: 2 Pages: 2000-2008
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: The objective of our study is to measure serum Malondialdehyde level(MDA), an index of oxidative stress, and Vitamin E level, a protective agent againstoxidative stress, in Jaundice patients and study the possible correlation between them .Materials and Methods: 80 patients with jaundice ( 38 female and 42 male) and 50 healthy control (23 female and 27 male) were enrolled in this study. About 10 ml of venous blood were obtained from the cubital vein using disposable needles and syringes. The thiobarbituric acid method was used to measure the Malondialdehyde (MDA) which reacted with thiobarbituric acid(TBA) to give pink color that was read at (535 nm). The concentration of vitamin E in serum was determined according to a modified Hashim and Schuttringer method.Results: A significant increase (P<0.0001) in serum Malondialdehyde (MDA) in jaundice patients as compared to that of normal healthy controls was observed. A significant decrease (P<0.0001) in serum vitamin E in jaundice patients as compared to that of normal healthy controls has been noticed. No significant correlation between serum Malondialdehyde (MDA) and serum Vitamin E in jaundice patients was observed.Conclusion: our data shows that antioxidant capacity decreases with an increase in oxidative stress in Jaundice patients but there is no significant correlation between them .Abbreviations: The abbreviations used are: dL, deciliter; MDA, Malondialdehyde; μl, microliter; r, Correlation factor ;rpm , Round per minute; TCA, Trichloroaceticacid; TBA, thiobarbituric acid ; ROS, Reactive oxygen species.


Article
Estimate of JAK 2 V617F Mutation and Activity of Catalase Enzyme in Myeloproliferative Neoplasm

Authors: Heba Rushdi Abdullah --- Perry Habib Saifullah --- Ziad Ahmed Shebib --- Ahmed Rushdi Abdullah
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2016 Volume: 19 Issue: 3 Pages: 1-8
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Myeloproliferative neoplasms (MPNs) are a range of clonal hematological diseases with overlapping features, a specific mutation in the JAK2 gene, which encodes a tyrosine Kinase has been shown to be associated with the myeloproliferative phenotypes (PV, ET and PMF). Aim: Estimate the proportion of JAK2 V617F mutant gene and estimate the significance of antioxidant enzyme (Catalase) is mainly associated with increased oxidative stress this is resulting increase of the free oxygen radicals and relate it with suspected myeloproliferative neoplasms (MPNs) in Iraqi patients. Materials and Methods: Total of (51) patients with suspected myeloproliferative neoplasms (MPNs) and 20 healthy individuals were analyzed for the JAK2 V617F mutation. After DNA extraction, detection of the mutation was done using (ARMS) PCR amplification, activity of Catalase was measured spectrophotometrically. Results: Of 51 patients, the JAK2 V617F mutation (V617F) was detected in 33 out of 42, with PV (81%), and four of patients with ET and PMF (40% and 50%, respectively). The prevalence of this mutation is more associated with male than female about (62%). Catalase activity was found highly significant (P< 0.01) among suspected MPN patients when compared with control group. Conclusion: JAK2 V617F mutation screening can be incorporated in the initial estimate of patients suspected of having MPNs. Catalase enzyme used a biomarker of enzymatic alteration in MPN Iraqi patients.

اورام التكاثر النقيي (MPNs) هي سرطانات تعرف باضطربات نسليه لانتاج الخلايا الدمويه مع زيادة إنتاج نوع واحد او اكثر من خلايا الدم الناضجة من انساب الدم النخاعي, و التي يمكن تصنيفها إلى: زيادة انتاج الكريات الحمراء الاولي (PV)، زيادة الاقراص الدمويه الأساسية (ET)، وتليف نخاع العظم الاولي (PMF).تهدف هذه الدراسة الى دعم تشخيص المرض بطريقة دقيقة وذلك بتقدير نسبة الطفرة الجينيه (JAK2 (V617F و قياس انزيم مضاد الاكسدة (الكاتليز) المرتبط بصورة اساسيه مع زيادة الاكسدة الاجهادية والناتجة من زيادة جذور الاوكسجين الحرة للمرضى المصابين بورم التكاثر النقيي (MPNs) وعند مقارنتهم مع الاشخاص الاصحاء, حيث تم تقدير نسبة الطفرة الجينية (JAK2 (V617F وقيمة انزيم الكتاليز عند (51) مريضا بورم التكاثر النقيي و(20) من الاشخاص الاصحاء.أظهرت نتائج الدراسة عند المرضى المصابين حديثا ان الطفرة الجينية( JAK2 (V617F هي إيجابية في 90٪ من مرضى زيادة كريات الدم الحمراء الاولي و 50٪ من مرضى تليف نخاع العظم الاولي و 40٪ من مرضى زيادة الاقراص الدموية الاساسيه. وظهر لدينا بوجود فرق معنوي عالي لمعدل قيم انزيم الكتاليز (0.01P<) عند المرضى بالمقارنة مع الاشخاص الاصحاء. استنتجت الدراسة الحالية ان طفرة الجين( JAK2 (V617F وجدت في 82% عند مرضى ورم التكاثر النقيي (MPN) وان المشتقات التفاعلية للاوكسجين له دور اساسي في التسرطن وتطور اورام التكاثر النقيي (MPNs).

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