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ELFA and IFAT Techniques to Detect Chlamydial Infections in Baghdad Women and Its Effect on the Immunoglobulins Level.

Authors: Khamael M. Kadaem* MSc --- Raad K. Al-Husseinei --- Lamya A. AL-Janabi --- Mun'im M. Fathei
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 192-197
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chlamydia trachomatis is one of the most common human pathogens and considered asone of the causative agents of STDs. This organism cause acute and recurrent pelvic infections andinfertility.Patients and Methods: Two hundred and seventy three females were included in the present study,attending infertility department, AL-Elwiya hospital, AL-Jarah private hospital, central public healthlaboratory and STDs clinic to whom IFAT, ELAF and immunoglobulins concentration were done.Results: Females were divided into three age groups <20; 20-39 and ≥40 years. Single and repeatedabortions were 44.9%, 55.1% respectively. Primary and secondary infertility were 55.6% and 44.4%.Higher abortions rate were in the age group 20-39 years which represents 31.5% and 41.6% in singleand repeated abortions. Primary and secondary infertility were high in the same age group whichrepresents 42.6% and 33.3% of the total investigated females, also the multipartners within the sameage group constitutes 56.7%.Chlamydial infections detected by IFAT technique constitute 12.8% whichwere represented as 14.6%, 11.1%, 36.7% and 5% in abortions, infertility, multipartners and fertilitywith no abortion groups respectively. While by ELFA technique the percentages were 12.4%, 9.3%,30% and 4% in the same mentioned groups.Immunoglobulins mean value in females with chlamydialinfections wee as follows IgG = 2102.1 mg/dl; IgA = 317.9 mg/dl and IgM = 272.5 mg/dl. Which weremore than the normal values of the immunoglobulins.Conclusions: Chlamydial infections were distributed largely among multipartners than other abortedfemales. High abortions were noted in age group 20-39 years. IFAT technique was more reliable thanELFA technique to detect chlamydial infections.IgG and IgM concentrations were higher than normal concentrations, while IgA remains normal.


Article
Prevalence Some Pathogenic Bacteria Causing UTI in Diabetic Patients In / Specialized Center For Endocrinology and Diabetes of Baghdad City–Iraq

Authors: Nihad Khalawe Tektook --- Khalid Ibrahim Al- Lehibi --- Raad K. Al-Husseinei
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 2 Pages: 260 -266
Publisher: Babylon University جامعة بابل

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Abstract

Urinary tract infections (UTIs) are caused by microorganisms but Bacteria are the most common cause when they overcome the natural defenses and causing infection, especially in patients with diabetes mellitus and its may present as asymptomatic bacteriuria, leading more serious infections. The aim of this study was to assess the prevalence of bacteria causing UTIs among diabetic patients. Collected one hundred and seventy five samples of sera and urine form diabetic patients with & without UTIs were studied from the specialized center for Endocrinology and diabetes All urine samples were tested by General urine examination (G.U.E) in addition to urine culture. On MacConkey and blood agar then identify the presence of the bacteria.Urinary tract infection increased with aging and increase percentage of UTI among individuals above 40 years so, high incidence of UTI among diabetic females (75%) rather that in diabetic males (25%). prominent UTI among females (68.8%) rather than males (31.1%) either diabetic or non-diabetic patients, as well as prevalence of smoking among males (74 %) than females (26%), So, Escherichia coli (15 isolates) most of them (13 isolates) in the urine sample of type II D.M particulary, the elderly ≥40 years females (11 isolates), and conclude from this study the duration of diabetes can affect the development of complication especially UTI complications, where their prevalence increases with longer duration of disease,Women with diabetes have more frequent and more sever UTI, and Escherichia.coli was the most common pathogen isolated in both groups.

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