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Article
Detection of Cytomegalovirus Infection in Pre-Marital Women in Al-Khalis City
تشخيص الفيروس المضخم للخلايا في النساء المقبلات عللى الزواج في الخالص

Author: Raghad Ibrahim Ahmed رغد ابراهيم احمد
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2017 Volume: 13 Issue: 4 - part 1 Pages: 117-125
Publisher: Diyala University جامعة ديالى

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Abstract

The study group included 100 blood samples pre-marital women in Diyala / Al-Khalis, form the period from 1/10/2015 to 1/4/2016, the blood sample of women without any clinical evidence of cytomegalovirus (CMV) infection and free from infected by HIV .we were screened for the presence of IgG and IgM antibodies against CMV by strip test.The IgG antibodies were detected in 10%, while the IgM antibodies were detected in (6%). The sero negative (control) was (90%) for IgG and (94%) for IgM. Rising in seropositivity was observed in young women in age group (25-30years) for both IgG and IgM, Seroprevalence of CMV was more in women from urban area IgG (80%), IgM(83%) with no significant result. Most of the women have higher education rising to 90% for IgG and 100% for IgM.

تضمنت مجموعة الدراسة 100 عينة من مصل دم النساء المقبلات على الزواج في محافظة ديالى / قضاء الخالص، استمرت الدراسه للفترة من 1102015إلى142016، حيث اخــــــذت عينة الدم من نساء اصحاء ظاهريا والعينات التي اخذت خالية من الاصابه بفيروس نقص المناعة البشرية .تم التحري عن وجود الفيروس بواسطه الشرائط الحاويه على الاجسام المضاده للفيروس المضخم للخلايا CMV-IgG وIgM CMV-. اظهرت النتائج ان نسبه الموجبيه للأجسام المضادة نوع IgG بنسبه 10% في حين الموجبيه للأجسام المضادة من نوع IgM كانت بنسبه (6٪) , بينما السلبية المصلية للـ(IgG) كانت(90٪) و (94٪) للـIgM. كما لوحظ ارتفاع الاستجابه المناعيه لدى الشابات ضمن الفئة العمرية (25-سنه30) لكل IgG وIgM، كما ظهر ارتفاع معدل الانتشار المصلي لدى النساء من المناطق الريفية اكثر من تلك التي في المدن وبنسبه (80٪)للـ IgG و (83٪) للـIgM من دون اي فرق معنوي يذكر. ارتفعت نسبه الاصابه في معظم النساء ممن لديهم التعليم عالى لتصل الى90٪ IgG و 100٪ IgM.


Article
Molluscum Contagiosum in Baquba City Epidimiological Study with Identification of Mc Viruses by Polymeras Chain Reaction

Authors: Khudhair Khalf Al-Kayalli --- Raghad Ibrahim Ahmed --- Brooj Mohammad Irzuqy --- Wathek Mohammed Husain
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2015 Volume: 8 Issue: 1 Pages: 8-13
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Molluscum contagiosum is a DNA virus, which may infect the mucous membrane, it is caused by Molluscipox virus and there are four types (MCV1-4). The sites of predilection of lesions are the face and neck, both genders equally affected and most commonly seen in childhood age group. Objectives: To study the epidemiological variables of Molluscum contagiosum and to confirm the clinical diagnosis and type of Molluscum cotagiosum virus (MCV) by Polymerase chain reaction (PCR) technique. Patients and methods: The present study was conducted for the period from 1st November 2011 to 30th April 2012 in outpatient clinic of Baquba teaching hospital in Baquba city. One hundred and sixty two patients with molluscum contagiosum were examined and diagnosed clinically and the diagnosis was confirmed by Polymerase chain reaction technique .The lesions of moluscum contagiosum were seen on different sites of the body, age of patients ranged from (1-80 years) with a mean age of 35+_9years . They were 114(70.6%) males and 48(29.4%) females.Results: The results showed that 72 (44.4%) patients of age group (31-40 years), of which 50 (70%) were males and 22 (30%) females, 35 (21.6%) of age group (ten years or less). This study revealed that 56(35%) of patients were illiterate, without statistical significant difference between MC infection and educational levels. In 78% of patients the lesions located on the head and neck, 70% on the right side and in 78% of patients the number of lesions was ≤10 lesions, 70% of patients lived in the urban areas and 30% in rural areas. Eighty five percent (85%) of patients give positive results for MCV, 58.8% of them shows positive results for MCV type 1 and 2 (73.3% MCV-2 and 26.7% MCV-1), while 41.2% shows negative results . Conclusion: We concluded that Molluscum contagiosum was a disease of adult, predominantly males and on the right side of the face and 73.3% of PCR positive results were MCV-2.

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