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Article
Transcriptional profiling of livers from different strain of mice treated with Asparaginase

Author: Rana Jaber Tarish
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2018 Volume: 17 Issue: 2 Pages: 125-128
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Asparaginase (ASNase) is widely used to treat acute lymphoblastic leukemia (ALL) in children but it causes metabolic complications related to liver toxicity. ASNase results in the synthesis of some factors such as ATF4. The eIF2-ATF4 pathway is essential for cell survival during amino acid starvation conditions. Activation of the AAR in the liver requires the eIF2 kinase called general control nonderepressible 2 kinase (GCN2). To what extent activation of the GCN2-eIF2-AAR is mediated by ATF4 is unknown. Our objective and hypothesis are addressed in our aim to describe the liver response to ASNase in mice deleted for Atf4. RNA sequencing alongside complementary biochemical approaches was performed in the livers of mice treated with eight daily injections of ASNase or saline excipient. Differences in gene expression were evaluated. We also explored the relationship between the different treatment groups and strains. This research provides insight into the importance of genetic background of patients in choosing ASNase as a treatment.

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Article
General Control Nonderepressible 2 and ATF4 Direct Liver Genes during Asparaginase Treatment in Mice

Author: Rana Jaber Tarish
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2018 Volume: 17 Issue: 2 Pages: 129-132
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Asparaginase (ASNase) treatment results in synthesis of some factors such as ATF4. The eIF2-ATF4 pathway is essential for cell survival during amino acid starvation conditions. Activation of the AAR in liver requires the eIF2 kinase called general control nonderepressible 2 kinase (GCN2). To what extent activation of the GCN2-eIF2-AAR is mediated by ATF4 is unknown. Our objective and hypothesis are addressed in our aim to describe the liver response to ASNase in mice deleted for Atf4. RNA sequencing alongside complementary biochemical approaches were performed in the livers of mice treated with eight daily injections of ASNase or saline excipient. Cellular pathways examined in detail included the AAR. We discovered that global hepatic gene expression patterns in Atf4 knockout mice overlapped with Gcn2 knockout mice. Shared hepatic pathways or processes altered during ASNase included mTOR signaling, and xenobiotic metabolism. On the other hand, loss of Atf4 during ASNase uniquely altered gene expression signatures reflecting signaling via eIF2 and ER stress. This research provides insight into the importance of genetic background of patients in choosing ASNase as a treatment

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Article
Physiological Effects of Zinc Oxide in Some of Reproductive and Blood Parameters in Female Rabbits
التأثيرات الفسلجية لأوكسيد الزنك في بعض معايير الخصوبة والمعايير الدموية لدى إناث الأرانب

Author: Rana Jaber Tarish Al-Baghdadi رنا جابر طارش البغدادي
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2011 Volume: 2 Issue: 2 Pages: 18-25
Publisher: University of Kufa جامعة الكوفة

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Abstract

Abstract: This study was performed to identify the physiological effects produced by addition of zinc oxide at 96 mg/ kg diet for 40 days in some of reproductive and blood parameters in female rabbits. Sixty local domestic rabbits of both sexes were used (20 males and 40 females), the males separated from females while females divided into two group, first group: (20 female) represented the control, the second group: (20 female) represented the treatment group in which ZnO2 added at 96 mg/ kg diet along the experiment period which lasted from 20/4/2008 to 30/5/2008, after 10 days from the beginning of the trail, mating occur between half males number (10) with the females of the control group and the other half of males with the females of treatment group, the males selected randomly (1male:2 females), then the males removed and the following parameters counted: gestational ratio, mean period of gestation, delivery ratio, mean of the numbers of offspring, the offspring mean weights, red blood cell level, white blood cell level and hemoglobin. The results revealed elevation in the above parameters in the females of treated group than the females of the control group.

الخلاصة: أجريت هذه الدراسة لمعرفة التأثيرات الفسلجية في بعض معايير الخصوبة وبعض المعايير الدمية لدى إناث الأرانب التي قد تنتج عن إضافة اوكسيد الزنك إلى علائقها بمقدار 96 ملغم/كغم علف لمدة 40 يوم. استخدم في هذه الدراسة 60 من ذكور وإناث الأرانب المحلية البالغة جنسياً (20 ذكر و40 أنثى)، عزلت الذكور على حدة أما الإناث فقد قسمت إلى مجموعتين مثلت الأولى (20 أنثى) مجموعة السيطرة، أما المجموعة الثانية (20 أنثى) فقد مثلت المجموعة المعاملة التي تم إضافة مادة اوكسيد الزنك إلى علائقها بمقدار 96 ملغم /كغم علف طيلة مدة التجربة التي استمرت من 20/4/2008 إلى 30/5/2008، بعد 10 أيام من بدء التجربة تم التزاوج بين نصف عدد الذكور(10) مع إناث مجموعة السيطرة والنصف الآخر من الذكور مع إناث المجموعة المعاملة، اختيرت الذكور عشوائياً (بواقع 1 ذكر:2 أنثى)، ثم استبعدت الذكور وتم حساب المعايير الآتية: النسبة المؤية للحمل، معدل مدة الحمل، النسبة المؤية للإنجاب, معدل عدد المواليد، معدل أوزان المواليد، معدل عدد كريات الدم الحمر، معدل عدد خلايا الدم البيض ومعدل مستوى خضاب الدم. أظهرت النتائج تفوق المعايير أعلاه في إناث المجموعة المعاملة عما هو عليه في إناث مجموعة السيطرة.

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Article
Nephroprotective roles of local licorice, peppermint extracts and their mixture on gentamicin-induced renal insufficiency in Wistar albino rats

Authors: Rana Jaber Tarish --- Ali M. Ghazi --- Jamela K. Abd-Alhassen
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 61-68
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Aminoglycosides (AGs) such as gentamicin (gen) are considered as the optimum therapy for many infections and diseases. Unfortunately, AGs treatment has been linked to acute kidney injury that is also referred to as nephrotoxicity. In the current study, we sought to explore the protective roles of peppermint (pep) and licorice (Lic) extracts as well as their mixture on gen-induced nephrotoxicity. Forty male and female Wistar albino rats were divided into 5 experimental groups: C- was administered with 2 ml of normal saline as a single daily dose intraperitoneally (i.p.) for 14 d. C+ was administered with once-daily i.p. injections of 100 mg/kg BW gen for 8 d. T1 group was administered once-daily i.p. of gen (100mg/kg BW) for 8 d. and Lic ethanolic extract (EE) (100mg/kg BW) every 12 hr for 14 d. T2 was administered once-daily i.p. of gen for 8 d. and pep EE (100mg/kg BW) every 12 hr for 14 d. T3 was administered once-daily i.p. of gen for 8 d. in addition to lic and pep EE mixture (100mg/kg BW) every 12 hr for 14 d. At the end of the study, all rats were anesthetized and trunk blood collected to study renal injury parameters. Those include serum enzymes such as AST, ALT, and ALP; kidney damage markers including urea, creatinine, and total protein; serum electrolytes including Cl, K, and Na levels as well as other renal function markers such as Glutathione peroxidase (GSH) and superoxide dismutase (SOD). All the parameters that indicated the nephrotoxicity occurrence in the C+ group in response to gen were found to be highly improved in most of the studied aspects in T1, T2, and T3. We found that Lic and pep extracts improve renal injury, kidney damage, and renal function markers opposed to gentamicin treatment. In conclusion, our study suggests prescribing those remedies to AGs receiving patients.

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Article
Deletion of GCN2 affects whole body and tissue response to asparaginase

Authors: Rana Jaber Tarish Al-Baghdadi --- Tracy G. Anthony
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 162-166
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Asparaginase (ASNase) treatment results in the synthesis of some factors such as activating-transcription-factor-4 (ATF4). The eIF2-ATF4 pathway is essential for cell survival during amino acid starvation conditions. This requires the eukaryotic-initiation-factor-2 (eIF2) kinase called general control nonderepressible 2 kinase (GCN2). Our objective and hypothesis are addressed in our aim to describe the liver response to ASNase in mice deleted for Gcn2 and Atf4 and either treated with phosphate buffered saline (PBS) or asparaginase (ASNase) for 8 days. We found that deletion of Gcn2 and/ or Atf4 affect body weight and fat and lean content. The results showed that Atf4-/- mice had significantly less fat mass than WT and Gcn2-/- mice even before the startup of the study. Also, WT mice experienced minimal change in body weight and body composition, but Atf4-/- and Gcn2-/- mice both lost substantial amounts of body weight and body fat without altering lean mass. Moreover, Gcn2-/- mice showed high significant increment in liver and pancreas weight when treated with ASNase compared to the other groups. Lastly, spleen weight was significantly lower in all treated groups, except Atf4 null mice, compared to their own control groups. In conclusion, this research provides insight into the importance of the genetic background of patients in choosing ASNase as a treatment.

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