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Morphometric study of kidney and suprarenal gland development after super ovulation injection in mice

Authors: Mohammad Oda Selman --- Ban Thabit Al-Ani --- Rana R. Al-Saadi --- Usama I. M. AL-Zubaidi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 6 Pages: 49-53
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Superovulation in mice causes a delayed embryonic development in vitro and in vivo, an increased abnormal blastocyst formation, pronounced fetal growth retardation.Objective:Morphometric measurements the dimensions (thickness, length) of fetal kidney and suprarenal gland during the later intrauterine life, after superovulation induction.Methods:Twenty fivematurefemalemiceSwiss-Webstermice ,thesemiceweredividedintotwogroups:experimental (G1) (15animal/group) and control group(G2) (10 animal/group). Superovulation was induced by intraperitoneal (IP) injection of 7.5 IU of PMSG ,then followed by 7.5 IU of HCG after 48 hours. Ovulation was taken place between 122± hours after injection of HCG. One mature male mice was placed with three superovulated female mice for mating. Pregnant females were sacrificedongestational day eighteen in all experimental groups. For morphometric study 20 mice embryos were fixedinBouin>sfixative,longitudinalsectionsofwholeembeddedembryowerestainedbyH&E,thespecimens independently read. The dimensions (thickness, length) of fetal kidney and suprarenal gland were evaluated.Results:The statistical analysis showed significantdecrease(P<0.01)inweightsoffetusesincomparisonwithcontrol group. The study was showed significantdecrease(P<0.01)infetalkidney dimensions atday 18 of intrauterine life, when it compared to the fetal kidney of non superovulated group(control group). There was also significantdecrease(P<0.01) inthemorphometricmeasurementsof fetalsuprarenal gland dimensions.Conclusion:The study improve that there is a delay in growth of kidney and suprarenal gland in fetal mice that belongs to mother undergo to superovulation induction before pregnancy.


Article
Role of alcoholic extract of Black Carrot in improvement of human sperm parameters during in vitro activation

Authors: Muhammad –Baqir M-R Fakhrildin --- Usama I. M. AL-Zubaidi --- Zena M Hussein --- Rana R. Al-Saadi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 1 Pages: 56-60
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundThe increasing prevalence of infertility cases is becoming a major public health problem in developing countries due to changes indiet and lifestyle.OhJectivesBlack Carrot Extract BCE (Daucuscarota) plays an important role as fertility agent due to its antioxidant and anti-proliferative properties. Therefore , the aim of the present study was to identify the effects of different concentrations of BCE on the outcome of in vitro human sperm activation of infertile patientsMethodsThirty two semen samples were prepared which served as a control, using direct swim-up technique. Modified Earl's medium (MEM) was used only in group 2 (G2) and two concentrations of BCE (1Oµg/1ml and 20µg/1ml) were added to culture media MEMof groups 3 (G3) and 4 (G4) for in vitro sperm activation (ISA).Resultsa highly significant (P<0.001) correlation was noticed between G4 with G2 and G3 in progressive motility when activation by adding 20µg/1ml of BCE to culture media as compared to G3 (which adding 1Oµg/1ml of BCE to culture media) and G2 (activation with culture media only).Moreover, swim-up activation technique with culture media and by both adding (1Oµg/1ml and 20µg/1ml of BCE respectively) to culture media enhancement in the (sperm motility, progressive sperm activity and sperm morphology).ConclusionThe high concentration of BCE is effective in the enhancement of progressive sperm activity during invitro human sperm activation.

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