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Article
COMPARISON BETWEEN SERUM PROLACTIN LEVELS DETERMINED BY VIDAS AND RIA TECHNIQUES
مقارنة بين مستويات مصل البرولاكتين مقاسا بتقنيتي الاشعاع المناعي والفلورة الرابط للانزيم

Authors: Yahya Y.Farid يحيى يحيى فريد زكي --- Rayah S. Baban ريا سليمان بابان
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2009 Volume: 7 Issue: 4 Pages: 20-26
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Human prolactin can be determined in human serum or plasma quantitatively by many techniques such as ELISA, RIA, and ELFA (miniVIDAS).Objectives: To estimate the strength of association of total serum prolactin and free serum prolactin values measured by two different methods [RIA and VIDAS]. And to predict the prolactin value measured by RIA corresponding to a given value by VIDAS.Methods: Two technical methods VIDAS and RIA were used in determination of prolactin level in sera of twenty five women with uterine fibroid conducted at two laboratories admitted at Al-Kadhimiya Teaching Hospital during the period January 2008 to April 2009. Total and free serum prolactin was measured by both VIDAS and RIA using their assay kits. Statistical methods of correlation and regression were used to compare between the two methods.Results: The study revealed a highly significant positive correlations between VIDAS and RIA total and free serum prolactin, r=0.999, R2 = 0.998, P<0.001, r=0.998, R2 = 0.997, P< 0.001 respectively. High linear regression equations were found between VIDAS and RIA total and free serum prolactin, y= 0.358x+ 0.57, R2 = 0.998 and y= 0.355x+0.49, R2 = 0.997 respectively. The recovery percentages of prolactin (R %) in two methods were approximately equal to each other, VIDAS R%=50.52±0.89% and RIA R%= 50.38±1.57% respectively.Conclusion: A highly significant positive correlation was found between RIA and VIDAS for both total and free serum prolactin. And high linear regression equations were found between the two methods to predict RIA values corresponding to a given VIDAS value.Keywords: VIDAS prolactin, RIA prolactin, PEG precipitation, macroprolactinemia, uterine fibroid

خلفية الدراسة : من الممكن تقدير مستوى هرمون البرولاكتين في مصل او بلازما الانسان كميا بواسطة العديد من التقنيات المختبرية كالتشخيص بالامتصاص المناعي الرابط للانزيمELISA، والتشخيص الاشعاعي المناعيRIA ، والتشخيص بالفلورة الرابط للانزيم ELFA (miniVIDAS).هدف الدراسة : لتخمين مقدار قوة الارتباط او الجمع لقيم البرولاكتين الكلي والحر في مصل الدم والتي تم قياسهم بطريقتين مختلفتين VIDAS و RIA .وكذلك لتوقع قيمة البرولاكتين المراد قياسها بطريقة الـRIA بمقابل قيمة معطى بطريقة ال VIDAS .طريقة العمل : تم استخدام تقنيتا الـ VIDAS و RIA في قياس مستوى البرولاكتين في مصل دم خمس وعشرين امراة تعاني من العقد الليفية الرحمية في مختبرين كانوا قد قدموا لمستشفى الكاظمية التعليمي خلال الفترة من كانون الثاني 2008 ولغاية نيسان 2009. قيس كل من البرولاكتين الكلي والحر في مصل دم المرضى بجهازي الـ VIDAS و الـ RIA باستخدام لوازم الطريقتين الخاصة بذاك الجهاز. كما استخدمت الطرق الاحصائية لعلاقتي الارتباط والارتداد للمقارنة بين هاتين الطريقتين.النَتائِــج : كشفت الدراسة عن وجود علاقة موجبة ذات إرتباطات إيجابية هامّة جداً بين مستوى البرولاكتين في المصل الكلي عند قياسه بكلتا التقنيتين كما هو في حالة مستوى البرولاكتين في المصل الحر عندما يقاس بنفس التقنيتين, r=0.999,R2=0.998, P<0.001 و, P<0.001 0.997= R2 و r=0.998 على التوالي. كما وجد ان هناك علاقة خطية ايجابية عالية بين مستوى البرولاكتين في المصل الكلي عند قياسه بكلتا التقنيتين كما هو في حالة مستوى البرولاكتين في المصل الحر عندما يقاس بنفس التقنيتين ، y= 0.358x+0.57 ، R2 = 0.998 و y= 0.355x+0.49 ، R2= 0.997 على التوالي. وعند حساب نسبة البرولاكتين المتبقية المئوية بكلتا الطريقتين كل على حدة وجد ان النسبتين تقريبا مقاربة لبعضهمها البعض VIDAS R%= 50.52±0.89% و RIA R%= 50.38±1.57 % على التوالي.الاستنتاج: وجد ت علاقة موجبة ذات إرتباطات إيجابية هامّة جداً بين مستوى البرولاكتين الكلي والحر في مصل الدم عند قياسهما بطريقة الـ VIDAS و الـ RIA . كما وجدت علاقة خطية ايجابية عالية بين كلتا الطريقتين لتوقع قيم البرولاكتيت بطريقة ال RIA مقابل قيم معروفة مقاسة بطريقة ال VIDAS .مفتاح الكلمات: برولاكتين الاشعاع المناعي، برولاكتين الفلورة الرابط للانزيم، العقد الرحمية، ترسيب البرولاكتين بمتعدد الاثلين الكلايكول


Article
ROLE OF ECTOPIC PROLACTIN ON THYROID HORMONES LEVEL IN A SAMPLE OF IRAQI INFERTILE WOMEN WITH UTERINE FIBROIDS

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Abstract

Background :A large number of traditional investigations and bioassay of hormones have been practiced in the diagnosis and management of infertility for a long time. Measurements of prolactin and thyroid stimulating hormone have been considered important components of the evaluation of women presenting with infertility.Objectives:To study the effect of prolactin hormone produced from uterine fibroid(s) on thyroid hormones levels and role of these hormones.Methods:One hundred three women with uterine fibroid were entered to the Obstetrics and Gynecology Department in Al-Kadhimyia Teaching Hospital in Baghdad-Iraq from the 15th of June 2007 to December 30th 2010. Fasting serum prolactin, total T3, total T4, and thyroid stimulating hormone (TSH) were estimated using MiniVIDAS [ELFA (Enzyme Linked Fluorescent Assay)] kits.Results:Forty five out of 103 (43.69%) women were found with primary infertility and the rest 58 (56.31%) with secondary infertility. Serum thyroid hormones mean ± standard deviations were found normal before and after surgery in both primary and secondary infertile women. Level of serum prolactin was found elevated about 9 folds in primary infertile women and 8 folds in those with secondry infertility before surgery more than their levels after. No significant difference was found between thyroid hormones before and after surgery in both infertile groups, unlike their prolactin which was found highly significant with p value <0.001 in both groups.Conclusion:It can be concluded that, first, the increase in prolactin level was due to an ectopic production from uterine fibroid(s), and second, there is no effect or role of this prolactin on their thyroid hormones function. Third, their infertility could be due to the presence of prolactin secreting fibroid(s).Keywords:Infertility, Ectopic prolactin, Thyroid hormones, Uterine fibroids


Article
ADSORPTION OF GLIMEPIRIDE ON ACTIVATED CHARCOAL AND IRAQI KAOLIN FROM AQUEOUS SOLUTION

Authors: Samir M Jasim سمير محمود جاسم --- Rayah S Baban ريا سليمان بابان --- Hiba S Jasim هبه سوادي جاسم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 1 Pages: 24-32
Publisher: Al-Nahrain University جامعة النهرين

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Background:Treatment of acute poisoning due to drug overdose in general depends on the prevention of further absorption of the drug and acceleration of elimination, using specific antidotes. Drugs adsorption is of significant importance in physical pharmacy for the preparation of physical antidotes.Objective:To investigate the adsorption of glimepiride from aqueous solution on two adsorbents (activated charcoal and Iraqi kaolin).Method:UV-Spectrophotometric technique was used to obtain the quantitive adsorption data at different conditions of pH and temperature.Results:The quantities of glimepiride adsorbed on activated charcoal and kaolin were increased with decreasing temperature. Adsorption isotherms of glimepiride on both surfaces were consistent with Freundlich adsorption isotherm. Thermodynamic functions (∆G, ∆H and ∆S) were useful in describing the spontaneity of the adsorption process.Conclusion:The quantity of the drug that is adsorbed on activated charcoal was higher than that adsorbed on kaolin surface therefore; the activated charcoal is a better antidote.Keywords:Glimepiride, Adsorption, Activated charcoal, Iraqi kaolin


Article
BODY MASS INDEX AND TOTAL SERUM LEPTIN LEVEL IN ABORTION

Authors: Nesreen A. Nasser نسرين احمد ناصر --- May F. Al-Habib مي فاضل ماجد الحبيب --- Rayah S. Baban ريا سليمان بابان
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 4 Pages: 361-367
Publisher: Al-Nahrain University جامعة النهرين

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Background:Adipose tissue secretes variety of adipokines, including leptin, which is involved in endocrine processes regulating reproduction and plays an important role in energy metabolism and fetal development during pregnancy.Objectives:To investigate the relationship between total serum leptin concentrations and anthropometric parameters including body mass index (BMI) in aborted women at the second trimester.Methods:A case control study was carried out from November 2011 to April 2012. The patients' group includes 30 aborted women at the second trimester. They were collected from Al-Elweyia, Al-Hakeem and Al-Khadhemiyia Teaching Hospital in Baghdad. Thirty healthy pregnant women (at their second trimester of gestation) were used as control. Patients and control were comparable in age. Blood HbA1c and serum concentrations of total leptin, lipid profile, and glucose were measured in both groups.Results:Total serum leptin concentration were significantly lower in those with abortion at the second trimester compared with healthy pregnant control (3.5±0.8 pg/ml vs. 24.4±0.7 pg/ml, P = 0.0001) and leptin/BMI ratio vs. control (0.1±0.02 vs. 0.9±0.01 ml, P=0.0001). No correlation was found between leptin level and patient’s age or gestational age in the case group. Highly significant correlation was found between patient’s total serum leptin and their body mass index, HDL-C, total cholesterol/HDL-C ratio and atherogenic index (P < 0.0001).Conclusion:The significant correlation between patient’s leptin and their BMI in addition to Leptin/BMI ratio even in non obese patient supports our objective that the unexplained abortion at second trimester is due to abnormality in their metabolic hormone action and reflect leptin resistance condition.Keywords:Second trimester abortion, Leptin, BMI, Gestational age.


Article
GHRELIN AND INSULIN RESISTANCE IN A SAMPLE OF IRAQI WOMEN WITH POLYCYSTIC OVARY SYNDROME

Authors: Amal A. Hussein امل علي حسين --- Rayah S. Baban ريا سليمان بابان --- Alaa G. Hussein علاء غني حسين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2014 Volume: 12 Issue: 1 Pages: 51-59
Publisher: Al-Nahrain University جامعة النهرين

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Background:Polycystic ovary syndrome is associated with adiposity and metabolic changes predisposing to insulin resistance and diabetes mellitus. Ghrelin is an appetite-stimulating hormone, which acts through its receptor on the hypothalamus to regulate energy balance and thus plays a major role in the etiology of metabolic diseases.Objective:To investigate the relation between ghrelin hormone and insulin resistance in patients with polycystic ovary syndrome.Methods:Thirty nine women with polycystic ovary syndrome and 30 healthy controls were examined. Fasting ghrelin, insulin, glucose, lipid profile concentrations were determined. Insulin resistance indexes were calculated (HOMA-IR and QUICKI-IR indexes).Results:Serum ghrelin concentration was significantly lower in polycystic ovary syndrome patients than control subjects (235 ± 17.36 pg/ml Vs 489.7 ± 53.4 pg/ml). Insulin resistance and BMI were significantly higher in polycystic ovary syndrome than control group.Conclusion:Ghrelin hormone may be used as a new additional marker in the diagnosis of polycystic ovary syndrome. Hyperinsulinaemia and hyperleptinemia are associated features in polycystic ovary syndrome.Keywords:Ghrelin, Obesity, Polycystic ovarian syndrome (PCOS), Body mass index (BMI), Insulin resistance


Article
A COMPARATIVE STUDY OF SERUM MALONDIALDEHYDE AND HEXANOYL-LYSINE ADDUCT IN PRETERM AND POST-TERM DELIVERIES

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Background:High oxidative stress reflects the state when the production of reactive oxygen species exceeds their removal. Malondialdehyde (MDA )and Hexanoyl-Lysine Adduct (HEL) are indicators of oxidative damage of lipids caused by free radicals in blood. The high level of these biomarkers has been implicated in early pregnancy complications.Objectives:This study aimed to investigate the correlation between serum oxidative stress biomarkers in preterm and post-term deliveries.Methods:A case-control study was designed to recruit 90 pregnant women with 30 women delivering at term (control group), 30 women at preterm (first case group) and 30 women at post-term (second case group). All women underwent elective cesarean section. Blood samples were collected before admission to operation theatre. Women's age, body mass index, lipid profile, renal function test and random glucose were measured. Serum oxidative stress biomarkers (Malondialdehyde and Hexanoyl-Lysine Adduct) were measured as indicators of lipid peroxidation.Results:The results showed that study groups were significantly different in serum MDA and HEL (P ≤ 0.01). Both serum MDA and HEL levels were significantly higher in preterm women group and their level steadily decreased as pregnancy progressed. Serum MDA was not significantly different between term and post-term women groups (P>0.05).Conclusions:Oxidative stress biomarkers may be important contributors of premature birth. Low level of serum HEL may play a role in delayed onset of labor. The causal relationship between oxidative stress biomarkers and pregnancy outcome may be further investigated by longitudinal studies.Key words:Oxidative Stress, Preterm women, Post-term women, Malondialdehyde, Hexanoyl-Lysine Adduct.

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