research centers


Search results: Found 10

Listing 1 - 10 of 10
Sort by

Article
Detection Immunohistochemical of P21 and P27 expression in Uterine Tumors.

Authors: Saad Hasan Mohammed Ali --- Basim Shehab Ahmed --- Sura Dhafer Dawood
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2017 Volume: 25 Issue: 2 Pages: 662-675
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Background:Several studies assessed gene and protein expression of p21 in endometrial carcinoma(EC), and mention that P21 represent an important participant in EC Cell invasion and metastasis ,while some researchers indicat that there were no apparent differences in immunostaining for p21.Other studies found that p27 expression significantly reduced in the endometrial carcinoma and inactivation ofP27 proteins is a specific feature in the progression of this cancer. Methodology: This study has used Immunohistochemistry for detection the gene expression of p21 and p27 in tissue specimens from 70 hysterectomized patients diagnosed with malignant uterine tumors (30 cases), non-malignant uterine tumors (25 cases),and 15 cases as control tissues groups.Results: The results of moleculare detection of P21 revealed12 (40%) in malignant uterine tumor 8(32%) non-malignant uterine tumor and 5(33.3%) in control groups and low expression of P27 revealed in all groups :5 (16.7%) in malignant uterine tumor 6(24%) non-malignant uterine tumor and 2(13.3%) in control groups. Conclusions: Decrease in expression of P21 was found mostly in malignant endometrial uterine tumors and this expressions occur could have correlated to the an early events of their tumorgenesis while low expression of P27 in hysterectomized patients mostly appear in non-malignant uterine tumors at the endometrial sites.

عدة دراسات قيمت التعبيرالجيني ل p21 في سرطان بطانة الرحم واشارت الى ان p21 يمثل مساهم مهم في اجتياح و انتقال سرطان بطانة الرحم, في حين اثبت بعض الباحثين عدم ظهور اختلاف في التصبيغ المناعي لل p21.وجدت دراسات اخرى اختزال ملحوظ في تعبير p27 وان التثيط في فعالية بروتين ال p27 صفة مهمة في تقدم هذا النوع من السرطان .طريقة العمل: اعتمدت هذه الدراسة تقنية Immunohistochemistry للكشف عن التعبير الجيني ل p21,p27 في عينات الأنسجة الماخوذة من 70 مريضة اجريت لها عملية اسئصال الرحم منها: (30 حالة) شخصت ضمن الأورام الخبيثة للرحم و(25حالة) ضمن أورام الرحم غير الخبيثة و( 15 حالة) كمجموعة انسجة السيطرة.النتائج: اظهرت الدراسة بان التعبير الجيني ل p21 كان (40 %( 12 من مجموعة أورام الرحم الخبيثة و(32٪) في ثمان حالات في المصابات بأورام الرحم غير الخبيثة .اما في أنسجة مجاميع السيطرة، تم الكشف عن خمس حالات فقط (33.3٪). اما التعبير الجيني ل p27 كان (16.7%(5 من مجموعة أورام الرحم الخبيثة و(24٪) في ست حالات في المصابات بأورام الرحم غير الخبيثة .و في حالتين(13.3%) من أنسجة مجاميع السيطرة.الاستنتاج: اغلب الانخفاض في التعبير الجيني ل p21 كان في مجاميع سرطانات الرحم الخبيث وممكن ان يكون له علاقة بالمراحل الاولى للسرطان,في حين p27 اظهر انخفاض في التعبير الجيني اغلبه كان ضمن مجاميع سرطانات الرحم غير الخبيث في بطانة الرحم.


Article
Evaluation of Performance Characteristics of Commercially Available Tests for Diagnosing Hepatitis B Surface Antigenemia

Authors: Saad Hasan Mohammed Ali --- Saja Jehad Al-Khalidi --- Waleed Nadi Kasim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 159-171
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:A number of serologic immunoassays techniques have been developed in diagnostic virology with different degrees of sensitivity and specificity for the detecting hepatitis B virus (HBV) antigens and their relevant antibodies.OBJECTIVE:This study was designed to apply and assess the sensitivity and specificity of different commercially available laboratory techniques for detecting hepatitis B surface antigen(HBsAg).METHODS:One hundred and twenty-one sera samples were collected from National Center for Blood transfusion, Gastroenterology and Liver Diseases Hospital, Central Public Health Laboratories and Teaching Laboratories. According to the manufacturer’s practical instructions, a group of commercially available laboratory methods for detecting HBsAg were applied , including enzyme linked immunosorbent assay (ELISA), enzyme linked fluorescent assay(ELFA), immunochromatographic assay(ICA), and latex agglutination test(LAT).RESULT:Among ELISA, ELFA, ICA, and LAT methods for detecting HBsAg, the 3rd generation ELISA was proved to have very high specificity (no false negative results) and the least one that has necessitated few confirmatory repetitions. ELFA versus ELISA has showed relatively lower sensitivity (more false negative results). However, similar to ELISA, ELFA showed very high specificity. Immunochromatographic assay (ICA) confidentially appeared to be a good, rapid and simple technique with comparable sensitivity and specificity to ELISA and ELFA techniques. Although LAT was introduced as a rapid, simple and cheap technique for HBsAg screening, it showed frank lower sensitivity and specificity that deranged it from competing with all those tested techniques.CONCLUSION:The concomitant use of ELFA with ELISA compensates its relatively lower sensitivity in front of ELISA. Latex agglutination test for HBsAg has relatively lower sensitivity and specificity than all other tests. For its comparable performance characteristics to ELISA, the use of ICA is ideally suited for HBsAg screening, in respect to its lower cost, rapidity, simplicity and no need for expensive equipments


Article
Localization of Human Cytomegalovirus- Late Gene DNA, Expression of P53 Gene and CD8-Tumor Infiltrating Lymphocytes in Oral Squamous Cell Carcinoma.

Authors: Saad Hasan Mohammed Ali --- Majed Mohammed Mahmood Al Jewari --- Noor Al Huda Ali A.H. Saaed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 2 Pages: 296-305
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT: BACKGROUND:Molecular DNA hybridization has confirmed an association of CMV infection with a variety of oralcancers. While cell mediated immunity is most important in controlling primary or reactivated CMVinfection, this virus has one of most effective strategies in oral carcinogenesis via impairment ofstructure and function of P53 protein by interaction with viraloncoproteins. OBJECTIVE:1.To determine the percentage of CMV-infected oral squamous cell carcinoma tissue specimens; 2.To evaluate the histopathological impact of the expression of mutated p53 tumor suppressor gene onCMV- related, as well as CMV-non-infected oral cancer; and 3.To through a light on someimmunological microenvironment of OSCC,by assessing CD8-positive tumorinfiltrating cytotoxicT-lymphocytes. METHODS:This study was designed as retrospective research. A total number of seventy (70) formalin-fixedparaffin-embedded oral tissues were collected; 60 patients with oral squamous cell carcinoma, and 10individuals with apparently-healthy oral tissues. The molecular methods for CMV DNA detection was performed by sensitive version of in situ hybridization, whereas the phenotype of cell surfaceantigen marker, namely CD8+ marker of tumor infiltrating lymphocytes and TP53 protein weredetected via relevant immunohistochemical methods. RESULTS: Well differentiated grade constituted 81.7% of oral squamous cell carcinoma. Positive in situ hybridization reactions for CMV-DNA were observed in 43.3% of the total screened tissues. Thirtythreeout of sixty (55%) oral squamous cell carcinoma showed positive immunohistochemicalreactions indicating P53 over-expression, and 18.3% showed presence of CD8-positive tumorinfiltrating lymphocytes. None of those control group showed positive reaction for CMV-DNA, p53,or CD8 marker.CONCLUSION:The detection of high percentage of cytomegalovirus-DNA in OSCC could mark for a parentral wayof spreading of such important and well-known sexually transmitted infection among Iraqi generalpopulation.The obvious high percentage of mutated p53 over-expression indicates for an importantrole of such genetic events in the oral carcinogenesis.A little role for CD8-positive tumor infiltratinglymphocytes could be played in the immunological microenvironment of OSCC


Article
Molecular Localization of Epstein Barr Virus and Rb Tumor Suppressor Gene Expression in Tissues from Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia
التموضع الجزيئي للتعبير الجيني لراشح الابشتاين بار والجين الكابت السرطاني Rb في أنسجة البروستات السرطانية والحميدة

Author: Saad Hasan Mohammed Ali 1 Shakir H. Mohammed Al-Alwany 2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2014 Volume: 13 Issue: 2-2 Pages: 161-172
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Epstein- Barr virus (EBV) is a ubiquitous in that infecting more than 90% of adult population worldwide. Recently, EBV has been linked to the development of variety of human malignancies including prostate tissues that range from benign prostatic hyperplasia (BPH) to prostatic adenocarcinoma (PAC). Somatic point mutations in Rb gene have been detected in prostate cancer and are involved in progression steps of prostate carcinogenesis. To analyze the distribution and impact of concordant Rb expression and latent EBV infection on a group of prostate adenocarcinoma and benign prostatic hyperplasia. Seventy- two formalin-fixed, paraffin- embedded prostatic tissues were obtained in this study; 40 biopsies from prostatic carcinoma and 20 from benign prostate hyperplasia as well as 12 apparently normal prostatic autopsies control group. Detection of EBV-EBERs was done by ultra-sensitive version of in situ hybridization method where as immunohistochemistry detection system was used to demonstrate the expression of Rb gene. Detection of EBV-EBERs -ISH reactions in tissues with PAC was observed in 19 out of 40 (47.5%), while in the tissues from BPH was detected in 10% (2 out of 20). No EBV-EBERs positive – ISH reaction was detected in healthy prostate tissues in the control group. The differences between the percentages of EBERs detection in tissues PAC and each of BPH & control groups were statistically highly significant (p < 0.01). Positive Rb immune histo chemical (IHC) reactions were observed in 19 PAC cases (47.5%) and in 2 BPH cases (10%). Our results indicate that the EBV might contribute to the development of subset of prostate tumors. In addition, the significant percentage of expression of possible Rb gene as well as EBV in prostate adenocarcinoma could indicate for an important role of these molecular and viral factors in prostatic carcinogenesis.

ايبشتاين- بار فيروس (EBV) فايروس يتواجد في كل مكان و يصيب أكثر من 90٪ من السكان البالغين في جميع أنحاء العالم. في الآونة الأخيرة تم ربط EBV في تطور مجموعة متنوعة من الأورام الخبيثة للإنسان بما في ذلك أنسجة البروستات التي تتراوح من تضخم البروستات الحميد (BPH) الى سرطان غدية البروستات الخبيث (PAC). تم الكشف عن الطفرات النقطية الجسمية في جينات الريتينوبلاستوماRB في سرطان البروستات والتي تشارك في خطوات تطور سرطان البروستات. الغرض من الدراسة عن الكشف عن علاقة ايبشتاين- بار فيروس (EBV) للإصابات الكامنة وتأثير تعبير جين الريتينوبلاستوماRB على مجموعة من مرضى سرطانات غدية البروستات وتضخم البروستات الحميد. تم الحصول على اثنين وسبعين عينة مريض لأنسجة البروستات المثبتة بالفورمالين، والتي مثلت عينات الدراسة وكالاتي: اربعون خزعه من سرطان غدية البروستات وعشرون خزعه من تضخم البروستات الحميد فيما مثلت اثنا عشر خزعه من انسجة البروستات للاشخاص السليمين كمجموعة سيطرة. تم الكشف عن ايبشتاين- بار فيروس (EBV) بوساطة طريقة التهجين الموقعي ذات الحساسية العالية. بينما تم استخدام تقنية الفحص الكيمائي المناعي النسجي للتدليل على تعبير جينات الريتينوبلاستوماRB . اظهرت نتائج الكشف عن ايبشتاين- بار فيروس (EBV) بوساطة طريقة التهجين الموقعي ذات الحساسية العالية (ISH) في انسجة سرطان غدية البروستات نسبة 47.5٪ (19 من 40 عينه)، بينما في انسجة بروستات الورم الحميد BPH)) كانت نسبة الإصابة 10٪ (2 من 20عينه). لا توجد نتائج موجبة للاصابة ب ايبشتاين- بار فيروس (EBV) في انسجة البروستات للأشخاص السليمين المستخدمين كمجموعة سيطرة. اظهر التحليل الاحصائي للفروق بين النسب المئوية للكشف عن ايبشتاين- بار فيروس (EBV) في انسجة سرطان غدية البروستات PAC وكل من وتضخم البروستات الحميد BPH ومجموعة السيطرة فرقا معنويا عاليا عند (0.01< P). كانت نتائج الفحص الكيمائي المناعي النسجي لسرطان غدية البروستات إيجابية بنسبة 47.5% (19من 40 عينه) وفي تضخم البروستات الحميد بنسبة10٪ (2 من 20 عينه) بينما في مجموعة السيطرة كانت النتائج سلبية.نتائج الدراسة الحالية تشير إلى أن EBV قد تسهم في تطوير فرعية من أورام البروستات. وبالإضافة إلى ذلك، يمكن للنسبة الكبيرة من التعبير الجيني لـ RB لهما دوران مهمان كعوامل فايروسية وجزيئية في تسرطن البروستات.


Article
Co-localization of EBV and Expression of P16- Cyclin-Dependent Kinase inhibitor Protein in Tissues from Patients with Non- Hodgkin's Lymphoma

Authors: Shakir H. Mohammed Al-Alwany --- Saad Hasan Mohammed Ali --- Masar Riyadh Rashid AL-Mousawi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2016 Volume: 9 Issue: 1 Pages: 2386-2396
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

background: EBV has been classified as a group 1 carcinogen associated with a variety of lymphoid and epithelial cancers. EBV was evidenced as a monogenic virus from its ability to transform normal human B cells, resulting in immortalization of the infected cells. A tumor suppressor protein, P16, is a cyclin-dependent kinase inhibitor plays a critical molecular role in cell senescence, regulation of the apoptosis pathway and G1 cell cycle arrest.Aim of the study: To analyze the concordant frequency and impact of P16 protein expression and EBV infection on tissues from a group of patients with Non- Hodgkin's Lymphoma (NHL).Patients and methods: Forty-five (45) formalin-fixed, paraffin- embedded tissues from lymph nodes biopsies were enrolled in this study; (30) lymph nodes biopsies were related to patients with NHL and (15) lymph nodes autopsies have included as apparently normal control group. Detection of EBV was done by ultra-sensitive version of in situ hybridization (ISH) method whereas immunohistochemical (IHC) system was used to demonstrate the protein expression of P16 tumor suppressor gene.Results: Detection of EBV -ISH reactions in tissues with NHL was observed in 17 out of 30 (56.7%), while in the tissues from lymph nodes autopsies was detected in 6.7% (1 out of 15).Positive P16- IHC reactions were observed in 14 out of 30 NHL cases (47.1%). No P16 positive – IHC reaction was detected in healthy lymph node tissues in the control group. The differences between the percentages of EBV and P16 detection in NHL tissues and control tissues group were statistically highly significant (P value = < 0.0001). Conclusions: The significant protein expression of P16 tumor suppressor gene as well as EBV infection in NHL in our results could indicate that cell cycle dysregulation and EBV-related transformation are important events in the pathogenesis of subset of NHL.


Article
Immunohistochemical Localization of Epstein Barr Virus- Latent Membrane Protein 1 in Breast Cancer Tissues

Author: Saad Hasan Mohammed Ali *,Shakir H. Mohammed Al-Alwany**, Ghanim I.A. Al-Wadi *
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 234-240
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACK GROUND:Epstein- Barr virus (EBV) is well known to infect the vast majority of the world's adult population and has recently been reported in many literatures for its association with a broad spectrum of benign and malignant tumors, including breast cancer. OBJECTIVE: To analyze the relations and impact of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) expression on a group of tissues with primary invasive breast cancers.PATIENTS AND METHODS: Forty- nine (49) formalin-fixed, paraffin- embedded breast tissues were obtained in this study; (34) biopsies from breast cancers (BC) and (15) from apparently normal breast autopsies control group. Detection of expression of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) was done by HRP/DAB immunohistochemistry detection kit (an immunoenzymatic antigen detection system for immunohistochemistry techniques) using specific primary antibodies for EBV-LMP 1.RESULTS: Detection of EBV-LMP 1 - immunohistochemical (IHC) reactions in tissues with BC was observed in 11 out of 34 (32.4%), while in healthy breast tissues in the control group was detected in 13.3% (2 out of 15). The difference between the percentages of EBV-LMP 1 detection in BC tissues & control group was statistically not significant (P >0.05).While no significant differences were observed between EBV-LMP 1 detection in BC tissues & the age of patients as well as grade of invasive breast cancers (P value > 0.05).CONCLUSION: Our results indicate that EBV could have a role in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis. .


Article
Molecular Detection of HPVgenotype-16 in a Group of Patients with Grade 1 Invasive Papillary Thyroid Carcinoma

Author: Saad Hasan Mohammed Ali*, Suha Abdullah AL-Fakhar*, Raad A. AL-Asady**, Shakir H. Mohammed***
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2015 Volume: 14 Issue: 3 Pages: 410-418
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND: Thyroid cancers are neoplasms originating from follicular thyroid cells thought to be related to a number of environmental and genetic predisposing factors. Human Papilloma Virus (HPV) is one among their associated infective agents.OBJECTIVE:To determine the frequency of human papilloma virus genotype-16 in infection in a group of iraqi patients with papillary thyroidcell carcinoma MATERIALS AND METHODS: A total number of 64 patients with different thyroid lesions were collected during 2008-2013; 30 have thyroid cancers, 22 benign thyroid tumors while12 healthy thyroid tissues were used as control group. In situ hybridization(ISH) was used to localize high risk HPV-genotype 16 in these tissues.RESULTS: Among malignant thyroid tumors 53.3% patients had HPV-16 while 27.3% HPV-16 positivity was detected in benign thyroid tumor group. None of healthy thyroid tissues revealed ISH reactions. There was 38.5% female had HPV-16, while there was 28% male had HPV-16. No significant statistical associations were noticed between the presence of HPV-16 and the age of those patients.CONCLUSION: Human papilloma virus genotype-16 could share a role in pathogenesis of this group of patients with thyroid cancers.


Article
P53-tumor suppressor gene overexpression in human papilloma virus-infected patients with oral squamous cell carcinoma

Authors: Saad Hasan Mohammed Ali سعد حسن محمد --- Athraa Yahya Al-Hijazi عذراء يحيى الحجازي --- Basim M.Khashman باسم خشمان
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2011 Volume: 23 Issue: special issue Pages: 70-76
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Via molecular DNA hybridization, more than 120 different genotypes of human papilloma virus havebeen confirmed. Many studies have described an association of high risk-HPV genotypes and overexpression ofmutated P53 gene with a variety of oral benign tumors as well as malignant squamous cell carcinomas. This studyaimed to: 1) Determine the prevalence of HPV DNA in archival tissue specimens with a range from apparentlyhealthy tissue to invasive oral S.C.C by using one of the recent versions of insitu hybridaization. 2) Define thegenotypes of the obtained HPV and to find out rational significance and relation of such genotypes to the severity ofunderlying lesions. 3) Study the correlation of over expressed products of mutant p53 genes with HPV-negative andHPV-related oral cancers.Materials and methods: A total number of 72 tissue specimens were collected from 41 patients with oral squamouscell (OSCC) and 31 individuals with apparently-healthy oral tissues (AHOT). The molecular detection methods for HPVdetection and genotyping were performed by in situ hybridization using cocktailed- and specified high- risk HPV DNAprobes, respectively. Immunohistochemical method was used to demonstrate the prevalence of P53 overexpressionin those oral cancers.Results: Among oral OSCC group, 16 archived tissue blocks were found to contain HPV DNA related to thecocktailed HPV genotypes. This result constituted 39% of the total oral SCC screened for HPV DNA. HPV-18 positiveoral SCC tissue blocks constituted (68.75%) whereas HPV genotypes 16& 31/33 constituted (43.75%) & (12.5%),respectively. Mixed infection of HPV genotypes was found in 31.3%. Interestingly, HPV DNA detection wasdocumented in 3.2% of those appeared as healthy tissues on histopathological examinations. Among oral SCCgroup, 22(53.7%) showed over expression of P53 tumor suppression gene. Interestingly, the co-occurrence of mutatedP53 overexpression and high oncogenic risk HPV genotypes was documented in 75% of Iraqi patients with OSCC.Conclusions: The significant prevalence of high oncogenic HPV genotypes detection in those patients with OSCCindicates a herald marks for the spread of such important sexually transmitted infection among Iraqi generalpopulation. Both of mutated p53 genes as well as high-oncogenic risk HPV genotypes could play an important role inoral carcinogenesis.


Article
Molecular Localization of Human Papilloma Virus genotypes (16, 18, and 6/11) in Patients with Colorectal Cancer by DNA- Insitu Hybridization
الموضعه الجزيئيه للفيروس الحليمي البشري ذو الانماط الجينية (16 و18 6 و11) في مرضى سرطان القولون والمستقيم باستخدام تقنيه الدنا التهجين الموضعي

Loading...
Loading...
Abstract

Human papilloma viruses (HPV) have been detected in several types of cancers. Over the last few years, a possible correlation between HPV infection and colorectal cancer has been suggested. The aim of this study was to investigate the association between human papilloma virus (HPV) infection and colorectal cancer. To determine the relationship between HPV and colorectal adenocarcinoma, a retrospective study was done. This study was carried out on 50 patients with hisopathologically confirmed primary colorectal cancer. Two samples were collected from each patient: one sample from the tumor site and the other one from adjacent normally appearing colorectal tissues, as well as ten (20) colorectal tissues from control individuals with no cancer. In situ hybridization (ISH) was used to detect HPV DNA (HPV 16 and 18 DNA ISH and 6/11 DNA CISH) in colorectal tissues. HPV 16 was detected in 16(32%) of tumor samples, and in 7 (14%) of adjacent normal tissues, and in additionally two cases of apparently healthy group gave positive results for it. HPV 18 was detected in 11 (22%) of tumor samples and in 6 (12%) of adjacent normal tissue. HPV 6/11 was detected in 24 (48%) of tumor samples and in 7 (14%) of adjacent normal tissue.Our results suggest that colorectal HPV infection is common in patients with adenocarcinoma colorectal, albeit at a low DNA copy number, with HPV16 being the most prevalent type. HPV infection may play a role in colorectal carcinogenesis.

تم الكشف عن فيروسات الورم الحليمي البشري في عدة أنواع من السرطانات. على مدى السنوات القليلة الماضية ، وقد اقترح وجود علاقة محتملة بين عدوى فيروس الورم الحليمي البشري وسرطان القولون والمستقيم.هدفت الدراسة الحالية لتحري عن العلاقة بين الاصابة بفيروس الحليمي البشري وسرطان القولون والمستقيم. لتَحديد العلاقةِ بين فيروس الحليمي البشري وسرطان القولون والمستقيم , صممت هذه الدراسة كبحث ذو أثر رجعي، اذ اشتملت على (50) مريضِ مَع اجراء الفحص النسيجي لتاكيد تشخيص سرطانَ القولون والمستقيم. تم جمع عينتان مِنْ كُلّ مريض: عيّنة واحدة مِنْ موقعِ الورمَ والآخرَ مِنْ الانسجة المجاورةِ التي تبدو سليمة، بالإضافة إلى (20) عينه من أنسجة القولون والمستقيم مِنْ أفرادِ غير مصابين ظاهريا كمجموعة سيطرة لهذه الدراسة. استخدمت تقنية التهجين الموضعي للكشف عن الدنا للفيروس الحليمي البشري ذو النوع الجيني 16 و18و 6/11 في انسجة سرطان القولون والمستقيم. ظهرت النتائج بان الفيروس نمط 16 وجد في 16حالة وبنسبة (32 %) من عيّنات الورمِ بينما في الأنسجةِ الطبيعيةِ المجاورة كانت 7 حالة وبنسبة (14 %)، وحالتلان فقط من مجموعه السيطرة اعطيت نتائج ايجابية. اكتشف الفيروس نمط 18 في 11 حالة وبنسبة (22 %) مِنْ الورمِ وفي 6 حالة وبنسبة (12 %) مِنْ النسيجِ الطبيعيِ المجاورِ. الفيروس الحليمي البشري نمط 6/11 إكتشفَ في 24 حالة وبنسبة (48 %) مِنْ انسجةالورمِ ووجد في 7 حالة وبنسبة(14 %) مِنْ النسيجِ الطبيعيِ المجاورِ.تَقترحُ نَتائِجَنا بأنّ الاصابة بفيروس الحليمي البشري شائعة في مرضى سرطان القولون والمستقيم، ولو أنْه عدد نسخ الدنا منخفض، الفيروس الحليمي البشري نمط 16 النوعَ الأكثر شيوعا». الاصابة بفيروس الحليمي البشري قد تلعب دور في تكون سرطان القولون والمستقيم.


Article
Association of CCND1 and HR-HPV16/18 in endo- metrial tumors
رابطة CCND1 و 18/ HR-HPV16 في أورام بطانة الرحم

Loading...
Loading...
Abstract

Background: A number of reports have demonstrated the presence of HPV in endometrial adenocarcinomas(ECs) . alterations in Cyclin D1(CCND1) gene expression has been reported in ECs and the integration of human papilloma virus (HPV)could alter the level of gene expression of CCND1. Methodology: This study has used in situ hybridization to localized HR-HPV16/18 in tissue specimens from 70 hysterecto- mized patients diagnosed with malignant endometrial tumors (30 cases), non-malignant endometrial tumors (25 cases),and 15 cases as control tissues groups. Also the study has enrolled detection the gene expression of CCND1 using Immunohis- tochemistry. Results:In the cancer sites malignant endometrial tumors group, HR-HPV16/18 was detected in 10 cases(33.3%) ,and in 5 cases (20%) non-malignant endometrial tumors ,and 4 cases (26.7%)of control tissues group. Most HPV16/18 infections have Punctate DNA pattern .The results of moleculare detection of CCND1 revealed 13(43.3%) in malignant endometrial tumor 8(32%) non-malignant endometrial tumor and 6(40%) in control groups. The signi cant correlation between CCND1 expression and HR-HPV16/18 infection was reported only in malignant endometrial tumors groups. Conclusions: HR-HPV16/18 may be associated with initiation of endometrial carcinogenesis events as well as play a role in the progression of such malignant tumors.CCND1 could have sharing in early events of tumorgenesis in endometrial carci- noma and a signi cant correlations of CCND1 expressions with HPV infection was observed among malignant endometrial tumor.

أظهرت عدد من التقارير وجود فيروس الورم الحليمي البشري في الاورام الغدية لبطانة الرحم.كما واظهرت الابحاث تغييرا في في الية التعبير الجيني ل Cyclin D1 في حا ت سرطان الرحم المصابة بالفايروس الحليمي البشري. استخدمت هذه الدراسة تقنية التهجين الموضعي لHPV في عينات ا نسجة الماخوذة من 70 مريضة اجريت لها عملية اسئصال الرحم منها: )30 حالة( شخصت ضمن ا ورام الخبيثة للرحم و)25حالة( ضمن أورام الرحم غير الخبيثة و) 15 حالة( كمجموعة انسجة السيطرة. وكانت نسبة الكشف عن HPV16/18 في مجموعة اورام الرحم الخبيثة 10 حا ت )٪33.3(، و5حا ت )20 %(من أورام الرحم غير الخبيثة، وايضا 4حا ت)٪26.7( من مجموعة أنسجة السيطرة.معظم ا صابات ب HPV16/18كانت ذات النمط المنقط من ال DNA. اما التعبير الجيني ل Cyclin D1،فقد تم الكشف عن 13حالة)%43.3( من مجموعة أورام الرحم الخبيثة و)٪32( في ثمان حالات في المصابات بأورام الرحم غير الخبيثة .اما في أنسجة مجاميع السيطرة، تم الكشف عن ست حا ت فقط )٪40(.كما واظهرت هذه الدراسة الجزيئية وجود ع قة بين ا صابة ب HPV16/18 وبين التعبير الجيني للCyclin D1 ضمن مجاميع اورام الرحم الخبيثة.اظهرت الدراسة ان ا صابة ب HPV16/18 قد تلعب دورا بتطور السرطان في بطانة الرحم وقد يكون لها دورا في تقدم المرض كما واظهرت الدراسة وجود ع قة معنوية بين ا صابة ب HPV16/18 وبين التعبير الجيني لل Cyclin D1ضمنمجاميع ا ورام الخبيثة لسرطان الرحم .

Listing 1 - 10 of 10
Sort by
Narrow your search

Resource type

article (10)


Language

English (10)


Year
From To Submit

2017 (1)

2016 (2)

2015 (3)

2014 (1)

2013 (1)

More...