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Article
Molecular Detection of Some Virulence Genes in Klebsiella pneumoniae Isolates from Patients with UTI Infection.

Author: Saba Saadoon Khazaal
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 4 Pages: 701 -705
Publisher: Babylon University جامعة بابل

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Abstract

Klebsiella pneumoniae causes a wide range of bacterial diseases like pneumonia, UTI and sepsis. Therefore, this study was done to assess the prevalence and molecular characteristics of K. pneumoniae in 24 samples of men and women isolated from Iraqi patients which was suffering from urinary tract infection (UTI) compared with (10) of healthy individuals. All samples were collected from Educational laboratories of Medical city/Iraqi Health Ministry. The samples were screened for the presence of kfu and k2A genes through PCR. The results of this study represented by (7 out of 24) isolates percent with (29.17%) positive for kfu gene and (9 out of 24) isolates (37.5%) were positive for k2A gene while the other (8) isolates were none of these genes. The two genes revealed significant differences between them (p< 0.5), so as with healthy group (p< 0.01). The study concluded that two genes kfu and k2A may affect on pathological of K. pneumoniae. The aim of this study represented by detection of kfu (iron uptake system gene) and k2A genes in patients with UTI which have a role in the pathogenicity of K. pneumoniae isolates.


Article
Detection Flagellin Gene of Enteric Isolates of Salmonella enterica serovarTyphi Using conventional PCR technique

Author: Saba Saadoon Khazaal
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2019 Volume: 30 Issue: 3 Pages: 9-11
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The study was conducted to study the virulence gene (Flagellin gene) of Salmonella typhi. Stool samples were collected frompatients with are ranging (15 – 60 ) years of the both sexes who suffering from diarrhea and visited AL- Yrmouk Teaching Hospital in Baghdad Governorate for the period betweenMarch to September 2017..The samples were cultured on suitable media then Api20 test was done in order to confirm the diagnosis, the polymerase chain reaction (PCR) was tested to diagnose the Flagellin gene responsible for the virulence of the bacteria. We conclude from the present study that flagellin gene in S.typhiis responsible for pathogenicity in this bacteria.

اأجريت هذه الدراسة لمعرفة جينات الضراوة لجراثيم سالمونيلا تايفي حيث تم جمع عينات الخروج من المرضى البالغين الذين تتراوح اعمارهم من (15 – 60) سنة ومن كلا الجنسينالذين يعانون من الإسهال والمراجعين لمستشفى اليرموك التعليمي في محافظة بغداد للفترة من شهر اذار الى شهر ايلول 2017 . حيث تم زرع العينات على الأوساطالزرعية المناسبة وبعدها تم عمل فحصApi20 وذلك لتأكيد التشخيص بعد ذلك تم اجراء فحص تفاعل البلمرة المتسلسلPCR لتشخيص جين الفلاجلين المسؤول عن ضراوة البكتيريا .نستنتج من هذه الدراسة وجود جين الضراوة الفلاجلين في جراثيم السالمونيلا تايفي والذي يعتبر مسوؤل عن الامراضية في هذه البكتريا .

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