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Article
Effect of Gamma Radiation on Pathogenecity of Entamoeba Histolytica

Authors: Hayam K. Al-Masoudi --- Zainab H. Al-Saadi --- Saif A. Al-Shalaah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 1 Pages: 301-309
Publisher: Babylon University جامعة بابل

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Abstract

The present study was conducted to test the effect of gamma radiation on the pathogenicity of E. histolytica in vivo by using radiation with doses (5, 10, 15, 20, and 25) rad to attenuation cyst of E. histolytica. The results revealed that mortality rate in laboratory animals (rabbits) decrease when increase the dose of attenuated E. histolytica cyst in comparative with positive control group (non – attenuated group) it reach (60% ,40%,40% and 20%) when give the rabbits attenuated E. histolytica cyst (5,10 ,15, and 20 ) rad. respectively . also we found no any mortality in rabbit which given attenuated E. histolytica cyst with dose (25) rad. also increasing doses of attenuating E. histolytica cyst (5,10,15,20 and 25) rad caused a some of histopathological changes in rabbits liver such as infiltration of inflammatory cell with degeneration of hepatic cells in addition to congested blood vessels and hydropic degeneration of hepatocyte in compare with liver of control group which gave the normal saline which appear normal shape and size. The results noted that this changes were reduce when increase the doses of radiation which decrease the pathogenecity of E. histolytica. We concluded that gamma radiation have good effects on reduce the pathogenicity of E. histolytica.


Article
Estimation of Immune Response in Rabbits Infected with Attenuated Entamoeba histolytica by Gamma Radation
تقييم الاستجابة المناعية في الارانب المصابة بالاميبا الحالة للنسيج المضعفة بواسطة اشعة كاما

Authors: Hayam K. Al-Masoudi هيام خالص المسعودي --- Zainb H.Al-Sadi زينب حمود السعدي --- Saif A. Al-Shalaah سيف انور الشلاه
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 3C Pages: 2529-2536
Publisher: Baghdad University جامعة بغداد

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Abstract

Out of 1279 stool sample only 245 were found to be infected with E.histolytica with total percentage 19% . Diagnostic study for E.histolytica by using techlab Eliza test showed that the non-pathogen E.dispar was significantly higher (78.9%) than E.histolytica (22%).We test the effect of the attenuated E. histolytica by gamma Radiation on the mortality rate in laboratory animals infected with E. histolytica by using increasing doses of radation (5,10,15,20and 25) Rad., the results showed that the percentage of mortality decrease when increase the dose of attenuated E.histolytica cyst in comparison with positive control group (non – attenuated group) it reached (100% ,66.7%, 33.3% 0% and 0%)respectively. Also we found that gamma radiation elevate some immunological parameters in laboratory animal. So the level of gamma interferon (IFN-γ) reached to (3000pg /ml) and level of ( IgE) reached to (550 mg/L) in comparison to control group ( 130.5 and 78.20 mg/L ) respectively . In conclusion ,the high level (IFN-γ) and (IgE) may be used as indicator for induction of immune responses which may have protective effect against amoebiasis infection.

من ما مجموعه 1279 عينة براز فقط 245 كانت موجبة للاميبا الحالة للنسيج بنسبة اصابة 19% . اظهرت الدراسة التشخيصية باستخدام اختبار Techlab Elisa test ان طفيلي E. dispar الغير مرضي كان اعلى نسبة اصابة (78.9%) من طفيلي الاميبا الحالة للنسيج (22%) . تم اختبار تاثير اشعة كاما في نسبة هلاكات الحيوانات المختبرية باستخدام جرع تصاعدية من اشعة كاما (5,10,15,20,25)% حيث اظهرت النتائج بان نسبة الهلاكات تقل عند زيادة جرعة الاشعاع بالمقارنة مع مجموعة السيطرة الموجبة (المجموعة غير المضعفة) حيث وصلت الى( and 0% 100%,66.7%,33.3%, 0%) على التوالي .كذلك وجد ان اشعة كاما رفعت بعض المعايير المناعية في الحيوانات المختبرية ، حيث ارتفع مستوى كاما انترفيرون الى (3000pg/ml) ومستوى IgE الى (550mg/L) بالمقارنة مع مجموعة السيطرة (130.5 و78.20) على التوالي . نستنتج من هذا ان المستوى العالي لمستوى IFN-γ) و (IgE دليل على حث الجهاز المناعي ضد الاصابة بالاميبا الحالة للنسيج.

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