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Diabetes Mellitus Correlation with Free Radicals and Creatine Kinase Isoenzymes Activity

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Abstract

To explain the relationship between free radicals production in experimentally diabetic animals and expected glutathione (GSH) reducing levels with its enhancement due to feeding of cysteine, N-acetyl cysteine and to evaluate the action of antioxidant to relief the damage to the active site of creatine kinase (CK) caused by free radicals, three doses during 48 hours of 150 mg alloxan / kg body weight was injected to forty eight males albino mice to produce the diabetes mellitus. Blood glucose and serum glutathione were determined using colorimetric methods, whereas, fractionation of CK isoenzymes in sera of diabetic mice was conducted by mini column ion exchange chromatography using Sephadex A-50. Agarose gel electrophoresis was used to quantitize the CK isoenzymes. Results of present study show that after long period of induced diabets in males albino mice cause a change in CK isoenzyme distribution pattern The total serum CK activity decreased in 75% of animals and reached to 63.5% of the control value whereas CK - MM isoenzyme was also decreased to 54.1% of its initial activity. Other isoenzymes showed variation depending upon the period of diabetes induced which caused elevated levels after 30 days of CK -BB isoenzyme in 62.5% of the animal studied and reached to 68% more than its initial activity which in < 3%. CK - MB which is found in 25% of albino mice was elevated to 17.1% more than that found in control sera which is < 3%. Insulin affects total CK activity and its isoenzymes distribution pattern as a therapeutic agent, and CK activity found to be elevated Thiol containing compounds including cysteine, N-acetyl cysteine dithiothreitol and glutathione have been suggested to reactivate the CK activity.

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