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Article
Silent Herpes Simplex virus infection in women with preterm premature rupture of membranes

Author: Shatha F.Abdullah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 4 Pages: 409-411
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: genital herpes simplex virus infection in pregnancy poses a major risk to the fetus and it has been associated with bad obstetric out come causing preterm labor, intrauterine growth retardation and spontaneous abortion. This study was conducted to determine if premature rupture of membranes before 37 weeks of gestational age are observed with subclinical shedding of herpes simplex virus (HSV).Patients and methods: Cervical swabs were taken from 75 women with a history of preterm premature rupture of membranes before 37 weeks of gestation, and 20 women with normal obstetrical history for the presence of HSV antigen using Enzyme linked Immuno Sorbent Assay (ELISA) method.
Results: HSV antigen was detected in 37 swabs(49.3%) which revealed a significant association with preterm premature rupture membranes(PPROM) compared to control group(P<0.05).The majority of HSV infected women were asymptomatic 34(91.9%), and 23(56.1%) of the cases was associated with history of recurrent PPROM, compared to those women with history of single PPROM.Conclusion: The risk of maternal transmission of HSV to the fetus or newborn is a major health concern and the high rates of undiagnosed or asymptomatic HSV infections complicate the issue of prevention. With advent of serologic test that can reliably detect the virus in asymptomatic patient. Maternal HSV screening now is mandatory.

Keywords

HSV --- preterm PROM --- maternal herpes.


Article
The Role of IL-1 and Tumor Necrosis Factor-α in Pathogenesis of Rheumatoid Arthritis

Authors: Eman Sh.Al-Obeidy --- Shatha F. Abdullah
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 2 Pages: 161-165
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Rheumatoid Arthritis (RA) is a common chronic inflammatory and destructive arthropathy that cannot be cured.Interlukin-1 (IL-1), IL-6 and tumor necrosis factor (TNF- α) are the key cytokines that drive inflammation in the disease.OBJECTIVE:The study was established to shed light on the possible role of IL-1 and TNF- α in pathogenesis of RA.PATIENTS AND METHODS:Rheumatoid Factors (RF),TNF-α and IL-1 were detected by Enzyme-Linked Immunosorbent Assay (ELISA) .C-reactive protein(CRP) on the other hand has been detected by using latex agglutination kit in the serum of 50 Iraqi patients with RA in comparison with 50 healthy individuals.RESULTS:Significant high levels of IL-1and TNF- α were observed in RA patients sera with P-value ( 0.04 and 0.001) respectively compared to healthy control group, in addition a positive linear association was found between the concentration of IL-1and TNF- α and CRP with P-value ( 0.05 and 0.001) respectively.CONCLUSION:IL-1and TNF- α play a role in pathogenesis of RA


Article
Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique

Authors: Ban A. Abdul majeed** PhD --- Faiza A. Mukhlis* PhD --- Shatha F. Abdullah* FICM
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 178-183
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can transform cells and dysregulate many cellular pathways involved in oncogenesis. This study was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion bodies in brain tumor tissues.Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks compared to eight normal brain autopsy specimens which were age and sex matched with the study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens representing (73.9% ) of the total study groups . All cases with high grades astrocytoma revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 ( 21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma cases(5.3 %), revealed inclusion bodies on histological examination. Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular studies are necessary to confirm the association of HCMV related human cancers in general Iraqi population.


Article
Hepatitis G virus infection among Iraqi patients with Chronic liver diseases

Authors: Faiza A.Mukhlis فايزة احمد --- Shatha F. Abdullah شذى فاروق --- Eman Sh.Al-Obeidy ايمان العبيدي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 3 Pages: 299-303
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The hepatitis G virus( HGV), also called hepatitis GB virus, as a member of the Flaviviridae family distantly related to hepatitis C virus (HCV), Little is known about the frequency of HGV infection, the nature of the illness, or how to prevent it. What is known is that transfused blood containing HGV has caused some cases of hepatitis. They infect humans, but are not known to cause human disease. This virus can be transmitted efficiently by blood transfusion and by other parenteral mechanisms. Transient and long lasting infections with HGV have been documented in man.Patients and methods: HBs Ag, Anti-HCV IgG and Anti-HGV IgG were detected by Enzyme-Linked Immunosorbent Assay (ELISA).HCV RNA on the other hand, has been detected using PCR technique in the serum of 75 Iraqi patients with chronic liver diseases in comparison to 15 healthy individuals.Results: HGV infection was detected in 25% of blood donors, 30% of chronic hepatitis C, 25% of chronic hepatitis B, and 20% of cryptogenic chronic liver disease. HGV infected patients tended to be younger than non-infected patients but no differences concerning sex, possible source of infection, clinical manifestations, biochemical and virological parameters, or severity of liver lesions were found.Conclusions: The percentage of HGV infection in chronic liver disease seems to be relatively high in our area 19 out of 90cases (21.11%). Infection with HGV does not seem to play a significant pathogenic role in patients with chronic liver disease related to chronic HBV or HCV infection, or in those with cryptogenic chronic liver disease. Key words: HGV, chronic liver disease, blood donors.


Article
Serological and molecular detection of parvovirus b19 in children with acute lymphoblastic leukemia

Authors: Mazin F. Al-Jadiry --- Shatha F. Abdullah شذى فاروق --- Iqbal M. Abdullah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2014 Volume: 56 Issue: 3 Pages: 318-321
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human parvovirus B19 (B19V) is the only human pathogenic parvovirus, It is highly erythrotropic and preferentially replicates in erythroid progenitor cells (EPCs). Recently the effects of B19 infection have been well studied in children with acute lymphoblastic leukemia (ALL). Objective: To detect parvovirus B19 among children with ALL.Methods: A cross sectional study involved forty five patients with ALL (21 patients were newly diagnosed ALL and 24 children who underwent chemotherapy) who were attending department of oncology in Children's Welfare Teaching Hospital in Baghdad Medical City between December 2012 and April 2013, compared to forty five of apparently healthy children as a control group, age and sex were matched. A serological and molecular detection of parvovirus B19 were done using enzyme linked immunosorbant assay (ELISA) and Real-time PCR.Results: B19-IgM, IgG and DNA were detected in study cases as (15.6%, 40% and 13.3%) compared to control group (4.4%, 13 % and 0%) respectively. B19-IgG and B19-IgM were detected in newly diagnosed cases in (41.7% and 19.05%) as well as in (38.1% and 12. 5%) of cases on chemotherapy respectively, all cases with B19-DNA signals were underwent chemotherapy (25%) with mean viral load of (36x104 copies/ml).Conclusion: children who suffering from ALL are at increased risk of B19 infection. Notable percentage of persistent B19 infection was recorded among children with ALL who are receiving ongoing treatment.Key words: parvovirus B19, acute lymphoblastic leukemia (ALL), real time PCR.


Article
Hepatitis G virus infection among Iraqi patients on maintenance hemodialysis
التهاب الكبد الفايروسي نمط ج بين المرضى العراقيين المستمرين على الديلزة الدموية

Authors: Ali A. Allawi علي عبد المجيد علاوي --- Shatha F. Abdullah شذى فاروق عبد الله --- Mohammad A. Al-Karkhy محمد عباس
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2014 Volume: 56 Issue: 1 Pages: 85-89
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Patients on maintenance hemodialysis are at increased risk of infection with parentally transmitted viral agents. In recent years a high prevalence of hepatitis G virus infection among end stage renal diseases and chronic hemodialysis patients has been well documented.Objectives: To assess the percentage and risk factors of HGV in hemodialysis patients, and to evaluate the clinical consequences of HGV in this population. Patients and methods: Fifty (50) patients with chronic renal failure who underwent maintenance hemodialysis. Patients were currently attending hemodialysis department of Baghdad teaching hospital during the period of October 2011 to January 2012, compared to forty one (41) healthy blood donors who underwent a full blood screening tests collected from blood bank. Aged matched as a control group. Anti-Hepatitis G antibodies (IgM and IgG) were detected using enzyme linked immunosorbant assay and HGV-RNA was determined by RT-PCR. Results: Hepatitis G virus -IgM and HGV-IgG were detected in 26 patients (52%) and in 36 patients (72%) respectively. HGV-RNA was detected in 16 patients (32%). Furthermore, nine (18%) patients revealed HGV-RNA bands and gave a positive HGV-IgM. Hepatitis G virus was significantly associated with the history and numbers of blood pints intake among hemodialysis patients, while there were no association with the hemodialysis duration, history of renal transplant nor with raised liver enzymes. Conclusions: Our results showed that hemodialysis patients carry the risk for HGV infection as a major possibility of parenteral transmission, especially by transfusion of blood and blood components. Decisions to screen blood supplies for a transfusion-transmitted infection agent should be based on sufficient benefits for recipients.Key words: HGV, maintenance hemodialysis, blood donors.

الخلاصةخلفية البحث:المرضى المستمرين على الغسل الكلوي الدموي هم أكثر عرضة للإصابة بالالتهابات الفايروسية المنقولة دمويا. في السنوات الحديثة تم توثيق انتشار كبير لالتهاب الكبد الفايروسي نمط ج بين مرضى العجز الكلوي ومرضى الغسل الكلوي الدموي المستمر.الأهداف:لتقييم نسبة وعوامل خطورة التهاب الكبد الفايروسي نمط ج عند مرضى الديلزة الدموية ولتقييم العواقب السريرية لالتهاب الكبد الفايروسي نمط ج.المرضى والطرق:اشتملت الدراسة على خمسين مريضا مصابين بالفشل الكلوي المزمن والذين يخضعون للغسل الكلوي الدموي بصورة مستمرة. المرضى كانوا يحضرون إلى ردهة الغسل الكلوي الدموي في مستشفى بغداد التعليمي للفترة ما بين تشرين الأول لعام 2011 ولغاية كانون الثاني لعام 2012, وبالمقارنة مع (41) فردا صحيا من متبرعين الدم الذين خضعوا للفحوصات المسحية الروتينية قد تم جمعهم من مصرف الدم واعتبارهم مجموعة الضبط. المضادات من الغلوبيولينات المناعية نوع M و G (IgM and IgG) لفايروس التهاب الكبد نمط ج قد تم تشخيصها باستخدام تقنية الروز المناعي المرتبط (ELISA) و الحمض النووي لفايروس التهاب الكبد نمط ج تم تحديده باستخدام تقنية عكس رد الفعل الناسخ البلمرة (RT-RNA).النتائج:المضادات من الغلوبيولينات المناعية نوع M و G قد تم الكشف عنها عند 26 مريضا (52%) و 36 مريضا (72%) بالتتابع. أما الحمض النووي لفايروس التهاب الكبد نمط ج فقد تم الكشف عنه عند 16 مريضا (32%). علاوة على ذلك, تسعة (18%) من المرضى اظهروا نتائج موجبة لوجود الحمض النووي لفايروس الكبد نمط ج و هم أيضا اظهروا نتائج موجبة للغلوبيولينات المناعية نوع M لفايروس الكبد نمط ج. التهاب الكبد الفايروسي نمط ج كان مرتبطا بشكل ملحوظ مع مرضى الغسل الكلوي الدموي والذين لهم تاريخ بتزويدهم مكاييل الدم وأعداد هذه المكاييل, بينما لم يكن هناك ارتباطا مع الفترة الزمنية للغسل الكلوي الدموي ولا التاريخ المتعلق بوجود عملية نقل الكلى أم لا ولا حتى مع ارتفاع انزيمات الكبد.الاستنتاجات:نتائجنا أظهرت ان مرضى الغسل الكلوي الدموي يحملون خطرا للإصابة بالتهاب الكبد الفايروسي نمط ج كاحتمال كبير من انتقاله دمويا, وخصوصا بواسطة عملية نقل الدم او مكوناته. لذلك الفحص المسحي لمزودات الدم التي تنقل وتعطي العامل المسبب للالتهاب يجب أن ترتكز لفائدة مستلمي الدم. مفتاح الكلمات : التهاب الكبد الفايروسي نمط ج, الديلزة الدموية المستمرة, متبرعي الدم.

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