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Determining the optimum conditions for bioemulsifier produced by Rhizobium leguminosarum biovar viciae isolated from Vicia faba root nodules
تحديد الظروف المثلى لانتاج المستحلب الحيوي من بكتريا Rhizobium leguminosarum biovar viciae المعزولة من العقد الجذرية لنبات الباقلاء

Author: Suaad Ali Ahmed سعاد علي احمد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 3C Pages: 2188-2196
Publisher: Baghdad University جامعة بغداد

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Abstract

The tested isolate of Rhizobium leguminosarum bv. viciae was isolated from root nodules of vicia faba plant from Baghdad ,the isolated bacteria was examined for bioemulsifier production when growing in mineral salt medium that containing 1% sunflower oil as sole carbon source . Rhizobium isolate was able to produce bioemulsifier with initial emulsification index E24% was 38%. Many of physical and nutritional cultural conditions for optimum emulsifier production was examined including : pH , temperature ,incubation period ,carbon and nitrogen source. The maximum bioemulsifier production form Rhizobium isolate was 64.5% when bacteria growing in mineral salt medium with pH 9 ,incubated at temperature 30 ºC , incubation period was 10 days ,the best carbon and nitrogen sources was sesame oil and NH4NO3 respectively .

تم عزل وتشخيص بكتريا Rhizobium leguminosarum biovar Viciae من العقد الجذرية لنبات الباقلاءVicia faba المؤخوذ من احد المزارع في بغداد, ومن ثم اختبار مقدرتها على انتاج المستحلب الحيوي عندما نميت في الوسط الانتاجي له وهو الوسط الملحي الحاوي على 1% زيت زهرة الشمس كمصدر كاربوني وحيد . وجد ان عزلة Rhizobium لها القدرة على انتاج المستحلب الحيوي وبمعامل استحلاب اولي emulsification index E24% مقداره 38% . تم اختبار عدد من الظروف الفيزيائية والغذائية لمزارع الانتاج لتحديد الظروف المثلى, والتي تضمنت الاس الهيدروجيني ,الحرارة , مدة الحضانة , والمصدر الكاربوني والنيتروجيني. بلغ اعلى انتاجية للمستحلب الحيوي من بكتريا العقد الجذرية Rhizobium leguminosarum biovar viciae بمعامل استحلاب emulsification index E24% مقداره 64.5% عندما نميت في الوسط الملحي الذي كان الاس الهيدروجيني له 9 ودرجة الحرارة 30 م° , وحضن لمدة 10 ايام . وكان افضل مصدر كاربوني وهيدروجيني على التوالي هو زيت السمسم ونترات الامونيوم.


Article
Increasing Cellulose Production from Rhizobium leguminosarum bv. viciae

Authors: Suaad Ali Ahmed --- Alaa Raheem Kazim --- Hussam Mahmood Hassan
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2017 Volume: 20 Issue: 1 Pages: 120-125
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Bacterial cellulose (BC) is a type of biopolymer produced as primary metabolism product by many genera of bacteria such as Acetobacter, Rhizobium, Agrobacterium, Psuedomonas and Sarcina. This article aimed to study the effect of some factors on cellulose production from Rhizobium leguminosarum bv. viciae which isolated from root nodules of Vicia faba plant collected from Abu-Ghraib in Baghdad. Some environmental and nutritional factors were examined including : carbon and nitrogen sources, pH, temperature and incubation period. Results showed that maximum production of cellulose was gained by Hestrin-Schram medium (HS medium) containing 2% fructose as carbon source, 1% yeast extract as nitrogen source, as well as optimum pH, temperature, and incubation period were (5, 30°C for 7 days) respectively .Cellulose dry weight was analyzed by Fourier transform infrared spectroscopy (FTIR) technique and the results showed the presence of the functional groups of bacterial cellulose. [DOI: 10.22401/JNUS.20.1.17]

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