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Article
Genotyping of Klebsiella spp. isolated from different clinical sources
التنميط الجيني لبكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة

Authors: Dalal S. Al- Rubaye دلال صالح الربيعي --- Heba Mohammed Hamza هبة محمد حمزة --- Thanaa R. Abdulrahman ثناء رشيد عبد الرحمن
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 3B Pages: 1937-1951
Publisher: Baghdad University جامعة بغداد

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Abstract

A total of 172 clinical were obtained over 6 months. Klebsiella spp. was detected in 58 (33.7%) samples with a high percentage 29 (50%) in urine in female and low percentage 1(1.7%) in pus and burn swabs in male, and the vaginal swab was 1(1.7%). The female to male ratio was 3.1:1. PCR detection showed that 51(87.93%) out of 58 produce 108 bp. product with rpoB specific primer that represented K. pneumonia. Whereas 7(12.07%) showed PCR product with 343 bp by K. oxytoca specific primer (peh X), furthermore, the sequences of two selected isolates showed that the species related to K. oxytoca strain CAV1335, and to K. oxytoca strain CAV1374. Five selected isolates were re-tested by the gyr A primer, all were showed specific band product with 441bp. Sequencing blast analysis for these isolates showed that one was related to K. pneumoniae subsp. pneumoniae strain RJF999, two isolates related to K. pneumoniae strain 17265 GyrA, one related to K. oxytoca strain 7102 GyrA and one related to K. pneumonia isolate 103 GyrA gene. Phylogenetic tree analysis showed the relation of 3 K. pneumoniae isolates to USA and UK strains and one with the Asian strains, and 2 K. oxytoca isolates have a relation within the Iranian strains and one has a genetic variation.

جمعت 172 عينة سريرية على مدى اكثر من ستة أشهر، وتم الكشف عن الكليبسيلا في 58 (33.7%) عينة وكانت اعلى نسبة 29 (50%) في عينات الادرار في النساء, واقل نسبة 1 (1.7%) من مسحات القيح والجروح في الدكور وبنفس النسبة في مسحات المهبل. وأشارت النتائج إلى أن نسبة عزلات الكليبسيلا في الإناث إلى الذكور هي 1:3.1. اظهر فحص انزيم البلمرة المتسلسل أن نسبة 51 (87.98%) من اصل 58 باستخدام بادئ rpo B قطعه بحجم 108 والتي تشير الى pneumoniae K. . بينما اظهرت 7(12.07%) قطعة بحجم 343 بأستخدام بادئ pehx الخاص ببكتيريا K.oxytoca وكان تطابق تسلسل القواعد النتروجينية لعزلتين منتخبتين منها الى انها ترجع الى K. oxytoca strain CAV1374و K. oxytoca strain CAV1335 تم اختبار خمس عزلات منتخبة باستخدام بادئ gyra واظهرت قطعة بحجم 441 باستخدام طريقة انزيم البلمرة المتسلسل للقواعد النتروجينة الى ان واحدة تعود الى K. pneumoniae subsp. Pneumonia strain RJF999 واثنين الى K. pneumonia strain 17265 GyrA وواحدة K.oxytoca strain 7102 GyrA وواحدة الى K.pneumonia isolate 103 . اظهر فحص شجرة العائلة الى ان ثلاث عزلات من K.pneumoniaقريبة بصفاتها من العزلات الامريكية والبريطانية وواحدة قريبة من صفات العزلات الاسيوية بينما كانت عزلتين K.oxytoca قريبة بصفاتها من العزلات الايرانية وعزلة واحدة مغايرة وذات صفات جينية بعيدة.

Keywords

K.oxytoca --- PCR --- sequencing --- phylogeny


Article
11.DETECTION OF SOME BIOFILM GENES RELATED WITH MULTIDRUG-RESISTANT IN ACINOBACTER BAUMANNII ISOLATED FROM CLINICAL ISOLATES

Authors: Sura S. Talib سرى سعد طالب --- Thanaa R. Abdulrahman ثناء رشيد عبد الرحمن --- Shatha H. Ali شذى حسين علي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 4 Pages: 430-438
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Acinetobacter baumannii (A. baumanii) has recently emerged as a major pathogen causing nosocomial infections in patients admitted to intensive care units with a surprisingly rapid acquisition of antibiotic resistance.Objective: To study the rate of occurrence of A. baumanii in different clinical samples and to investigate the association between biofilm formation and presence of ompA and bap genes in multi-drug resistance isolates.Methods:A total of 150 clinical samples were collected from (blood, sputum, urine, wound swab) during a period from the first of October 2017 to the end of March 2018 from Al-Imamein Al-Kadhimein City, Central Teaching Hospital of Pediatrics, Welfare Children Protection in Medical City and Al-Yarmouk Teaching Hospital and tested against 14 antibiotics by disc diffusion method. Quantitative microtiter plate assay was done for detection of biofilm formation. Polymerase chain reaction (PCR) was performed to detect ompA and bap genes.Results: There were 75 A. baumanii isolated from different clinical samples as follows: 41 from blood, 13 from wound, 12 from sputum and 9 from urine. The results of antimicrobial susceptibility test showed, high rate of resistance to Aztronem (94.7%) followed by Cefotaxime (89.3%), Cefepim (86.7%), Meropinem (86.7%), Ceftriaxone (86.7%), Ceftazidime (85%), Gentamicin (85%), and Piperacillin (82.7%) respectively. Moderate - to - low rate of resistance to Ciprofloxacin (78%), Impenim (46.7%), Levofloxacin (46%), Amikacin (44%), Tigacycline (42.3%) and Colistin (44%). The detection of biofilm formation showed that (52%) of isolate produce biofilm and the prevalence of ompA gene was 86.7% while the prevalence of Bap-gene was 34.7%.Conclusion: High frequency of A. baumannii infection was observed in different hospitals in Baghdad. More than half of the isolates were biofilm producer and there is highly significant association between the presence of bap gene and the biofilm formation but not with ompA gene.Keywords: Acinetobacter baumannii, ompA, Bap, MDCitation: Talib SS, Abdulrahman TR, Ali SH. Detection of some biofilm genes related with multidrug-resistant in Acinobacter baumannii isolated from clinical isolates. Iraqi JMS. 2018; 16(4): 430-438. doi: 10.22578/IJMS.16.4.11

Keywords

Acinetobacter baumannii --- ompA --- Bap --- MD


Article
EVALUATION OF PLASMID-MEDIATED QUINOLONE RESISTANCE ASSOCIATED WITH THE QNR GENES IN CLINICAL ISOLATES OF SHIGELLA SPP. IN BAGHDAD

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Abstract

Background:Although quinolone resistance results mostly from chromosomal mutations in Enterobacteriaceae, it may also be mediated by plasmid-encoded qnr determinants. Shigella harboring the novel qnr plasmid-mediated mechanism of quinolone resistance has been described worldwide.Objective:To understand the distribution of serogroup of Shigella spp, as well as antimicrobial susceptibility and to investigate the plasmid mediated quinolone-resistant qnr genes in clinical isolates of Shigella spp. resistant to quinolone.Methods:Fifty nine clinical isolates of Shigella spp. were collected from two hospitals in Baghdad. Antimicrobial susceptibility tests were performed using disk diffusion test and minimum inhibitory concentration. The isolates were screened for the plasmid-mediated qnr genes of qnrA, qnrB, and qnrS by Multiplex polymerase chain reaction.Results:The isolation rate of Shigella spp. was 14% and observed to be high among children < 10 years and low in teenagers and adults. The highest percentage was Sh. flexneri (54.2%) followed by Sh. sonnei (37.3%) then Sh. dysenteriae (8.5%), while no Sh. boydii was found in this study. Antimicrobial susceptibility tests revealed that 54.23% and 49.2% of both Sh. flexneri and Sh. sonnei were resistant to nalidixic acid and ciprofloxacin, respectively, while Sh. dysenteriae isolates were fully susceptible to these antibiotics. The minimum inhibitory concentration value of resistant isolates of Sh. flexneri and Sh. sonnei ranged between 2-64 μg/ml and 32-512 μg/ml for ciprofloxacin and nalidixic acid, respectively. Multiplex polymerase chain reaction amplification of plasmid-borne qnrA, qnrB, qnrS genes revealed that the overall percentage of qnr-genes were (52.9%) distributed as (29.4%) qnrA, (20.6%) qnrS and (2.94%) qnrB detected alone or in combination. The genes were identified in (44.1%, 15/34) of quinolone resistance Shigella isolates.Conclusion:To our knowledge, this is the first report detected fluoroquinolone resistance due to the qnr gene among Shigella isolates in Iraq which is indicated that plasmid-mediated quinolone resistance has emerged in Iraqi pediatric patients.Keywords:Drug resistance, Shigella spp., Plasmid; Quinolone, qnr

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