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Evaluation of antibacterial activity of cinnamon and ginger extracts against vancomycin-resistant Staphylococcus aureus isolated from nose of food handlers in restaurants and cafeterias
تقييم فعالية المضاد البكتيري لمستخلصات القرفة والزنجبيل ضد المكورات العنقودية الذهبية المقاومة للفانكومايسين والمعزولة من انف المتعاملين مع الاطعمة في المطاعم والكافيتريات

Author: Uday Abdul-Reda Hussein عدي عبد الرضا حسين
Journal: Thi-Qar Medical Journal مجلة ذي قار الطبية ISSN: 19929218 Year: 2017 Volume: 14 Issue: 2 Pages: 75-93
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Background: Food handlers are the main source of Staphylococcal food poisoning in developed countries.Objective: The aim of this study was to determine the prevalence of the nasal carriage of vancomycin resistant Staphylococcus aureus among food handlers in restaurants and cafeterias, and to evaluate of antibacterial activity of cinnamon and ginger extracts against these bacteria.Material and Methods: A total of 100 nasal swabs were collected from healthy food handlers and analyzed for Staphylococcus aureus ,methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Staphylococcus aureus (VRSA) using standard methods. Aqueous and ethanolic extracts of each plant were prepared by using a Soxhalet apparatus and the antibacterial activities of these extracts against VRSA were determined by using agar well diffusion method.Results: of 100 nasal swabs, 30 (30%) isolates were Staphylococcus aureus , among which 25 (83.3%) isolates were MRSA, and 20 (80%) isolates among MRSA were VRSA. Both plant extracts showed potent antibacterial activity against VRSA. Cinnamon extracts showed higher antibacterial activity when compared with ginger extracts , whereas ethanolic extract of each plant was more effective than that of aqueous extract.Conclusion: This study revealed a relatively high prevalence rate of VRSA nasal carriage among food handlers. Cinnamon and ginger are rich source of antimicrobial compounds which can be used as natural food preservatives and to treat infections caused by these bacteria .


Article
Role of Eruca sativa in prevention of induced nephrocalcinosis in rabbits
دور نبات الجرجير في منع التكلس الكلوي المحدّث في الأرانب

Author: Uday Abdul-Reda Hussein عدي عبد الرضا حسين
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2018 Issue: 14 Pages: 65-73
Publisher: Kerbala University جامعة كربلاء

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Abstract

Background: Nephrocalcinosis is a clinical pathologic condition characterized by abnormal deposition of calcium phosphate or calcium oxalate in the renal parenchyma, which can result in impaired kidney function.Aim: This study aimed to evaluate the effect of Eruca sativa aqueous extract in prevention of nephrocalcinosis induced by a large dose of oxalic acid in rabbits. Materials and Methods: Twenty four healthy domestic rabbits were equally allocated into three groups and were distributed into: normal group (without drug), oxalic acid group and Eruca sativa group. Both the first and second groups were received distilled water, while the third group received aqueous extract of Eruca sativa for ten consecutive days. Single high dose of oxalic acid was given to induce nephrocalcinosis in rabbits of the second and third groups (except normal group) after 2 hours from receiving of distilled water and Eruca sativa extract at the first day. Blood samples were collected from all animals for biochemical analysis. Urine analysis and histopathological examination were performed for all rabbits to verify the presence of crystals in urine and renal tissues. Results: Eruca sativa aqueous extract produced highly significant reduction in blood urea nitrogen, serum creatinine , with significant reduction in serum Na+ , and highly significant elevation in serum Ca2+ and serum K+ levels, also it is produced highly significant reduction in the calcium oxalate crystals density in urine and renal tissues when compared with of oxalic acid group. Conclusion: Current study found that the aqueous extract of Eruca sativa effectively prevented the formation and deposition of calcium oxalate crystals in renal tissues, thus preventing the occurrence of nephrocalcinosis.

التكلس الكلوي، حامض الاوكزاليك، فرط أوكسالات البول، مستخلص نبات الجرجير، بلورات أوكسالات الكالسيوم.


Article
Antimicrobial activity of ethanolic and aqueous extracts of pomegranate peel against Extended Spectrum Beta-Lactamase producing bacteria
فعالية المستخلصات الايثانولية والمائية لقشور الرمان ضد البكتريا المنتجة لأنزيم البيتا لاكتميز الواسع الطيف

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Abstract

Background: Pomegranate (Punica granatum ) is an ancient, mystical, unique fruit with potential antimicrobial activity.Objective: This study was performed to explore the antimicrobial activity of ethanolic and aqueous extracts of pomegranate peel against extended spectrum beta-lactamase producing bacteria (Klebsiella pneumonia , Escherichia coli and Aeromonas hydrophila). Material and Methods: 100 swabs were collected from the skin of burned patients. The bacterial strains were identificated according to their cultural , morphological , microscopically and biochemical characteristics, then subjected to ESBL-producing screening by double-disc synergy test. The ethanolic and aqueous extracts of pomegranate peel were prepared by using a Soxhalet apparatus and their antimicrobial activities were studied and compared with a commercial antibiotics against tested bacteria by using agar well diffusion method.Results: Out of total 100 swabs, only three isolates produced ESBLenzyme, namely (Klebsiella pneumoniae, Escherichia coli and Aeromonas hydrophila). Both extracts showed an effective antibacterial activity against all these bacteria. The ethanolic extract was found to be more effective than aqueous one against all the tested microorganisms.Conclusion: The pomegranate peel extracts have a strong antimicrobial activity against extended spectrum beta-lactamase producing bacteria. Therefore it is an important source of new antimicrobial compounds to treat infections caused by multidrug-resistant bacterial.

الهدف: اجريت هذه الدراسة لتقييم فعالية المستخلصات الايثانولية والمائية لقشور الرمان ضد البكتريا المنتجة لأنزيم البيتا لاكتميز الواسع الطيف.طريقة العمل: تم جمع 100 مسحة من جلد الاشخاص المصابين بالحروق, ثم اجريت عدة اختبارات للبكتيريا المعزولة لتحديد نوعها و قابليتها لإنتاج انزيم البيتا لاكتميز الواسع الطيف. تم تحضير المستخلصات الايثانولية والمائية لقشور الرمان باستخدام جهاز السوكسليت, ثم استخدمت طريقة الاكار لتحديد تأثير المستخلصات على هذه البكتيريا.النتائج: من مجموع مائة مسحة , تم الحصول على ثلاث عزلات بكتيرية منتجة لانزيم البيتا لاكتميز الواسع الطيف وهي Aeromonas hydrophila, E. coli Klebsiella pneumonia , أظهر المستخلص الايثانولي والمائي نشاطا فعالا عاليا ضد هذه البكتريا, حيث وجد أن المستخلص الإيثانولي أكثر فعالية من المستخلص المائي.الاستنتاج: اظهرت مستخلصات قشور الرمان نشاطا فعالا عاليا ضد البكتريا المنتجة لانزيم البيتا لاكتميز الواسع الطيف, والذي يمكن ان يستخدم كعقار ضد البكتيريا المقاومة للعديد من الادوية وعلاج الالتهابات الناجمة عنها

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