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Article
Impact of Vinblastine Sulphate on sperm parameters and histological changes of the seminiferous tubules in mice

Author: Usama I. M. AL-Zubaidi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 6 Pages: 44-48
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Vinblastine Sulphate (VBL) is administered with other anticancer drugs for treatment of metastatic testicular carcinoma.Objective:The aim of this study was to investigate the effects of different concentrations of vinblastine on the outcome of sperm parameters and histological changes of the seminiferous tubules in mice.Methods:Thirty fivehealthymaturemalemice(78-) weeks old and weight of (2325-) g were involved in the present study. They were divided into six treated groups (0.092, 0.14, 0.18, 0.23, 0.28 and 0.4 mg/kg) with control group (5 mature males mice/group) according to periods of orally administration of each week throughout 6 weeks. Males were scarifiedattheendofadministrationperiodforassessmentofsperm function parameters. The testes were obtained and prepared for histological sections. Diameter of seminiferous tubules and thickness of layers of proliferated spermatogenic cells were assessed for all groups of male mice.Results:A significantdecrease(P<0.05)wasobservedinthespermconcentrationaftertreatmentwiththedoses 4, 5 and 6 when compared with control group and highly significantdecrease(P<0.001)inthesperm viability percentage was appeared for all doses groups when compared with control group. On the other hand, highly significantdecrease(P<0.001)appearedinthespermmotilityaftertreatmentwith all doses when compared with control group.Diameter of seminiferous tubules and thickness of proliferated spermatogenic cells layers were decreased gradually throughout periods of orally vinblastine administration. Highly significantdecrease(P<0.001) in the diameter of seminiferous tubules and thickness of proliferated spermatogenic cells layers was appeared following the treatment with high doses when compared with control.Conclusion:High doses of vinblastine sulphate have negative effects on sperm parameters and histological feature testis in mice.


Article
Influence of different concentrations of Sildenafil Citrate on histological parameters of mice epididymis
تأثير تراكيز متعددة لسترات السلدينافيل على المتغيرات النسجية لبربخ الفئران

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Abstract

sildenafil citrate (SC) is widely used for the treatment of erectile dysfunction and maintains a sufficient erection for satisfactory sexual performance. Very limited literatures were published about effects of SC on histological changes of epididymes in human or laboratory animals. The aim of this study was to investigate the influence of different concentrations of SC for different administration periods on some histological parameters of mice epididymes. Seventy five male mice (7-8) weeks old, weighing (23-25)g were used in the present study. The possible effect of SC was tested through the treatment groups of mice with different doses of SC (2, 4, 8, 16)mg/Kg body weight, and for different periods (1, 3, 5) weeks. Histological examination of epididymes were carried out and compared with those belong to untreated group. Results revealed no significant differences (P>0.05) in epididymal weight, tubules diameters and thickness of epithelial layer between treated groups and control group. Conclusion in this study, SC has no effect on epididymis histology

أستخدم عقار سترات السلدينافيل (الفياغرا) بشكل واسع لعلاج فشل الانتصاب لدى الذكر وكذلك ادامة الانتصاب للحفاظ على الكفاءة الجنسية . فقد نشر عدد قليل جداً من المصادر حول تأثيرات سترات السلدينافيل على التغيرات النسجية للبرابخ في الانسان والحيوانات المختبرية . لذلك هدفت هذه الدراسة الى التحقق من تأثير تراكيز مختلفة لسترات السلدينافيل لفترات تجريع مختلفة على بعض المتغيرات النسجية لبرابخ الفئران . شملت هذه الدراسة خمس وسبعون ذكر فأر بعمر (7-8) اسابيع وبوزن (23-25)غم ولمعرفة تأثير سترات السلدينافيل التي أختبرت خلال المجاميع المعاملة للفئران تم تقسيم الذكور الى مجاميع اعتماداً على تراكيز سترات السلدينافيل المختلفة (2 ، 4 ، 8 ، 16(ملغم/كلغم ولفترات مختلفة (الاسابيع الأول والثالث والخامس) . وبانتهاء فترة التجريع أخذت البرابخ خارج جسم الحيوان وتم تحضيرها للمقاطع النسيجية . أظهرت نتائج الدراسة الحالية فروقات غير معنوية في أوزان البرابخ وأقطار نبيبات البرابخ وفي سمك طبقة الخلايا الطلائية لنبيب البربخ بين مجموعة السيطرة وجميع مجاميع المعاملة . نستنتج من نتائج هذه الدراسة ان التجريع بسترات السلدينافيل لم يعطي تأثيرات على انسجة بربخ الفئران .


Article
Morphometric study of kidney and suprarenal gland development after super ovulation injection in mice

Authors: Mohammad Oda Selman --- Ban Thabit Al-Ani --- Rana R. Al-Saadi --- Usama I. M. AL-Zubaidi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 6 Pages: 49-53
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Superovulation in mice causes a delayed embryonic development in vitro and in vivo, an increased abnormal blastocyst formation, pronounced fetal growth retardation.Objective:Morphometric measurements the dimensions (thickness, length) of fetal kidney and suprarenal gland during the later intrauterine life, after superovulation induction.Methods:Twenty fivematurefemalemiceSwiss-Webstermice ,thesemiceweredividedintotwogroups:experimental (G1) (15animal/group) and control group(G2) (10 animal/group). Superovulation was induced by intraperitoneal (IP) injection of 7.5 IU of PMSG ,then followed by 7.5 IU of HCG after 48 hours. Ovulation was taken place between 122± hours after injection of HCG. One mature male mice was placed with three superovulated female mice for mating. Pregnant females were sacrificedongestational day eighteen in all experimental groups. For morphometric study 20 mice embryos were fixedinBouin>sfixative,longitudinalsectionsofwholeembeddedembryowerestainedbyH&E,thespecimens independently read. The dimensions (thickness, length) of fetal kidney and suprarenal gland were evaluated.Results:The statistical analysis showed significantdecrease(P<0.01)inweightsoffetusesincomparisonwithcontrol group. The study was showed significantdecrease(P<0.01)infetalkidney dimensions atday 18 of intrauterine life, when it compared to the fetal kidney of non superovulated group(control group). There was also significantdecrease(P<0.01) inthemorphometricmeasurementsof fetalsuprarenal gland dimensions.Conclusion:The study improve that there is a delay in growth of kidney and suprarenal gland in fetal mice that belongs to mother undergo to superovulation induction before pregnancy.


Article
Role of alcoholic extract of Black Carrot in improvement of human sperm parameters during in vitro activation

Authors: Muhammad –Baqir M-R Fakhrildin --- Usama I. M. AL-Zubaidi --- Zena M Hussein --- Rana R. Al-Saadi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 1 Pages: 56-60
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundThe increasing prevalence of infertility cases is becoming a major public health problem in developing countries due to changes indiet and lifestyle.OhJectivesBlack Carrot Extract BCE (Daucuscarota) plays an important role as fertility agent due to its antioxidant and anti-proliferative properties. Therefore , the aim of the present study was to identify the effects of different concentrations of BCE on the outcome of in vitro human sperm activation of infertile patientsMethodsThirty two semen samples were prepared which served as a control, using direct swim-up technique. Modified Earl's medium (MEM) was used only in group 2 (G2) and two concentrations of BCE (1Oµg/1ml and 20µg/1ml) were added to culture media MEMof groups 3 (G3) and 4 (G4) for in vitro sperm activation (ISA).Resultsa highly significant (P<0.001) correlation was noticed between G4 with G2 and G3 in progressive motility when activation by adding 20µg/1ml of BCE to culture media as compared to G3 (which adding 1Oµg/1ml of BCE to culture media) and G2 (activation with culture media only).Moreover, swim-up activation technique with culture media and by both adding (1Oµg/1ml and 20µg/1ml of BCE respectively) to culture media enhancement in the (sperm motility, progressive sperm activity and sperm morphology).ConclusionThe high concentration of BCE is effective in the enhancement of progressive sperm activity during invitro human sperm activation.

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